scholarly journals Loss of pyrethroid resistance in newly established laboratory colonies of Aedes aegypti

2019 ◽  
Author(s):  
Farah Z. Vera-Maloof ◽  
Karla Saavedra-Rodriguez ◽  
Rosa P. Penilla-Navarro ◽  
Americo D. Rodriguez-Ramirez ◽  
Felipe Dzul ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Natural Populations ◽  
Resistance Management ◽  
Target Site ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Frequent Use

AbstractBackgroundResistance to pyrethroid insecticides in Aedes aegypti has become widespread after almost two decades of their frequent use to reduce arbovirus transmission. Despite this, use of pyrethroids continues because they are relatively inexpensive and because of their low human toxicity. Resistance management has been proposed as a means to retain the use of pyrethroids in natural populations. A key component of resistance management assumes that there is a negative fitness associated with resistance alleles so that when insecticides are removed, resistance alleles will decline in frequency. At least three studies in Ae. aegypti have demonstrated a decrease in pyrethroid resistance once the insecticide is removed.Methods/Principal FindingsThe present study aims to evaluate variation in the loss of pyrethroid resistance among newly established laboratory populations of Ae. aegypti from Mexico. Eight field collections were maintained for up to eight generations and we recorded changes in the frequencies of mutations at the V1,016I locus and at the F1,534C locus in the voltage gated sodium channel (VGSC) gene. I1,016 and C1,534 confer resistance. We also examined resistance ratios (RR) with type 1 and 2 pyrethroids.Conclusions/SignificanceWe demonstrate that, in general, the frequency of the Ae. aegypti pyrethroid resistance alleles I1,016 and C1,534 decline when they are freed from pyrethroid pressure in the laboratory. However, the pattern of decline is strain dependent. In agreement with earlier studies, RR was positively correlated with I1,016 resistant allele frequencies and showed significant protection against permethrin, and deltamethrin whereas F1534C showed protection against permethrin but not against deltamethrin.Author SummaryThe author is interested in the evolution of genes that confer resistance to insecticides, especially when this evolution affects binding of insecticides to their target site. The Voltage Gated Sodium Channel gene represents an excellent opportunity to understand how mutations at the target site(s) affect the evolution of resistance in many different pest insect species including Aedes aegypti, the primary vector of Dengue Virus, Yellow Fever, Zika and Chikungunya arboviruses.

scholarly journals A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti

2007 ◽  
Vol 16 (6) ◽  
pp. 785-798 ◽  
Author(s):  
K. Saavedra-Rodriguez ◽  
L. Urdaneta-Marquez ◽  
S. Rajatileka ◽  
M. Moulton ◽  
A. E. Flores ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Latin American ◽  
Pyrethroid Resistance ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals Detection of Polymorphism on Voltage-gated Sodium Channel Gene of Indonesian Aedes aegypti Associated with Resistance to Pyrethroids

2018 ◽  
Vol 10 (3) ◽  
pp. 250-5 ◽  
Author(s):  
Budi Mulyaningsih ◽  
Sitti Rahmah Umniyati ◽  
Tri Baskoro Tunggul Satoto ◽  
Ernaningsih Ernaningsih ◽  
Dwi Aris Agung Nugrahaningsih
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Single Amino Acid ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene ◽  
Yogyakarta City

BACKGROUND: Aedes aegypti is a vector of several pathogens including dengue virus. Vector control is an effective way to break the transmission but unfortunately constant use of insecticides ultimately causes vector resistance. Pyrethroids have been used for about 15 years to combat Ae. aegypti in Yogyakarta Province, Indonesia. Single amino acid substitutions in the voltagegated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance (kdr). The aim of this study is to detect resistant status to cypermetrine and polymorphism on the voltagegated sodium channel gene of Ae. aegypti from 2 dengue endemic areas in Yogyakarta Province (Yogyakarta city and Sleman district).METHODS: Pyrethroid resistance in Ae. aegypti mosquitoes was detected by using CDC Bottle Bioassay. To detect the polymorphism on the voltage-gated sodium channel gene of Ae. aegypti analyses were conducted by using PCR and direct DNA squencing with primers AaSCF1 and AaSCR4 for S989P, I1011M (or V), L1014F sites, and AaSCF7 and AaSCR7 for the F1534C site.RESULTS: According to bioassay, the results for Ae. aegypti from Yogyakarta city (93% mortality) and Sleman district (88% mortality) suggest the possibility of resistance to cypermethrin. We observed polymorphism on voltagegated sodium channel gene on site F1534C (heterozygous).CONCLUSION: The findings provide early evidence that the use of cypermethrin (pyrethroids) in Yogyakarta city and Sleman district, Yogyakarta Province, Indonesia is reducing its effectiveness to control Ae. aegypti. Recommendations include additional tests for confirmation.KEYWORDS: Aedes aegypti, dengue virus, cypermethrin, Bioassay, Yogyakarta

A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance

2010 ◽  
Vol 96 (3) ◽  
pp. 127-131 ◽  
Author(s):  
Jintana Yanola ◽  
Pradya Somboon ◽  
Catherine Walton ◽  
Woottichai Nachaiwieng ◽  
La-aied Prapanthadara
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Point Mutation ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations

2019 ◽  
Author(s):  
Kentaro Itokawa ◽  
Koji Yatsu ◽  
Tsuyoshi Sekizuka ◽  
Yoshihide Maekawa ◽  
Osamu Komagata ◽  
...  
Keyword(s):  
Amino Acid ◽  
Insecticide Resistance ◽  
Sodium Channel ◽  
Genomic Dna ◽  
Genomic Structure ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Probe Set ◽  
Capture Sequencing

AbstractInsects’ voltage-gated sodium channel (VGSC) is the primary target site of pyrethroid insecticides. Various amino acid substitutions in the VGSC protein are known to confer insecticide resistance and are selected under insecticide pressure. In the genome, the VGSC gene consists of more than 30 exons sparsely distributed across a large genomic region, which often exceeds 100 kbp. Due to this complex genomic structure of gene VGSC, it is usually challenging to genotype full coding nucleotide sequences (CDSs) of VGSC from individual genomic DNA (gDNA). In this study, we designed biotinylated oligonucleotide probes via annotated CDSs of VGSC of Asian tiger mosquito, Aedes albopictus. The probe set effectively concentrated (>80,000-fold) all targeted regions of gene VGSC from pooled barcoded Illumina libraries each constructed from individual A. albopictus gDNAs. The probe set also captured all homologous VGSC CDSs except tiny exons from the gDNA of other Culicinae mosquitos, A. aegypti and Culex pipiens complex, with comparable efficiency by virtue of the high conservation of VGSC at the nucleotide level. Furthermore, we developed an automated bioinformatic analysis pipeline to genotype VGSC after capture sequencing—MoNaS (Mosquito Na+ channel mutation Search)—which conducts mapping of reads, variant calling, and variant annotation for nonsynonymous mutations. The proposed method and our bioinformatic tool should facilitate the discovery of novel amino acid variants conferring insecticide resistance on VGSC and population genetics studies on resistance alleles with respect to the origin, selection, and migration of both clinically and agriculturally important insect pests.

scholarly journals KÊNH DẪN TRUYỀN NA+ CỔNG ĐIỆN ÁP (NaV) VÀ TÍNH KHÁNG THUỐC DIỆT CÔN TRÙNG Ở MUỖI TRUYỀN BỆNH SỐT XUẤT HUYẾT

2017 ◽  
Vol 15 (3) ◽  
pp. 393-401
Author(s):  
Nguyễn Thị Kim Liên ◽  
Nguyễn Thị Hương Bình
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Aedes Albopictus ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated

Bệnh sốt xuất huyết là bệnh truyền nhiễm cấp tính, có thể gây thành dịch lớn và có tỷ lệ tử vong cao. Bệnh lưu hành trên hơn 100 quốc gia trên thế giới ở hầu hết các châu lục. Sốt xuất huyết được lây truyền qua vector trung gian là muỗi Aedes (Aedes aegypti và Aedes albopictus). Việc kiểm soát vector truyền bệnh đóng vai trò quan trọng trong việc ngăn ngừa sự bùng phát của dịch. Trong các biện pháp kiểm soát vector truyền bệnh, việc sử dụng thuốc diệt côn trùng đã mang lại những hiệu quả đáng kể và thuốc diệt côn trùng được sử dụng rộng rãi hiện nay thuộc nhóm pyrethroid. Các hóa chất thuộc nhóm pyrethroid chủ yếu tác động lên côn trùng thông qua các thụ thể trên kênh dẫn truyền Na+ của các neuron thần kinh ở côn trùng. Pyrethroid cản trở sự ngừng hoạt động của kênh dẫn truyền, kết quả là kênh K+ mở trong thời gian dài làm gián đoạn tín hiệu điện trong hệ thống thần kinh, làm mất khả năng bay của côn trùng. Tuy nhiên, khả năng kháng thuốc ở côn trùng trong đó có muỗi đang khiến cho hiệu quả của thuốc diệt côn trùng bị giảm đi. Khả năng kháng thuốc được xác định là do các đột biến trên gen mã hóa cho kênh dẫn truyền Na+ (voltage-gated sodium channel – VGSC). Cho đến nay, rất nhiều đột biến đã được xác định có liên quan đến tính kháng ở các quần thể muỗi Aedes. Thêm vào đó, tần xuất của các đột biến trên gen VGSC ở các quần thể muỗi khác nhau có sự khác biệt rất lớn. Vì vậy, nghiên cứu tính kháng thuốc diệt ở muỗi Aedes sẽ giúp cho việc kiểm soát tốt đối với vector truyền bệnh sốt xuất huyết.

Sign in / Sign up

Export Citation Format

Share Document