Genetic engineering of virus resistance in plants

1989 ◽  
Vol 26 (1) ◽  
pp. 105-106
Author(s):  
David Baulcombe
Author(s):  
B. D. Harrison

SynopsisSome of the most successful early applications of genetic engineering in crop improvement have been in the production of virus-resistant plants. This has been achieved not by the transfer of naturally occurring resistance genes from one plant species or variety to another but by transformation with novel resistance genes based on nucleotide sequences derived from the viruses themselves or from virus-associated nucleic acids. Transformation of plants with a DNA copy of the particle protein gene of viruses that have positive-sense single-stranded RNA genomes typically confers resistance to infection with the homologous and closely related viruses. Transformation with a gene that is transcribed to produce a benign viral satellite RNA can confer virus-specific tolerance of infection. In addition, recent work with viral poly-merase gene-related sequences offers much promise, and research is active on other strategies such as the use of virus-specific ribozymes.Already the field trialling of plants incorporating transgenic virus resistance has begun, with encouraging results, and effects on virus spread are being studied. Deployment strategies for the resistant plants must now be devised and the conjectural hazards of growing them assessed. Genetically engineered virus resistance promises to make a major contribution to the control of plant virus diseases by non-chemical methods.


2020 ◽  
pp. 115-137
Author(s):  
J. Muthuvel ◽  
Manalisha Saharia ◽  
Sanjeev Kumar ◽  
Moses Akindele Abiala ◽  
Gundimeda J. N. Rao ◽  
...  

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 449-456 ◽  
Author(s):  
Rebecca Grumet

2013 ◽  
Vol 115 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Ralph Scorza ◽  
Ann Callahan ◽  
Chris Dardick ◽  
Michel Ravelonandro ◽  
Jaroslav Polak ◽  
...  

2002 ◽  
pp. 581-584
Author(s):  
H. Raquel ◽  
C. Silva ◽  
R. Batista ◽  
A. Margarida Santos ◽  
T. Lourenço ◽  
...  

1990 ◽  
Vol 115 (1-2) ◽  
pp. 1-21 ◽  
Author(s):  
F. Gadani ◽  
L. M. Mansky ◽  
R. Medici ◽  
W. A. Miller ◽  
J. H. Hill

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 572a-572
Author(s):  
Rebecca Grumet

One of the first major successes in the genetic engineering of useful traits into plants has been the engineering of virus resistance. The first example of genetically-engineered virus resistance was published in 1986, since then there have been more than 50 reports of genetically engineered plant virus resistance. These examples span a range of virus types, a variety of plant species, and have utilized several different types of genes. A unique feature of the genetically-engineered virus resistance is that the resistance genes came from the virus itself, rather than the host plant. Most examples have utilized coat protein genes, but more recently, replicase-derived genes have proved highly effective. Other strategies include the use of antisense or sense-defective sequences, and satellite or defective interfering RNAs. This talk will provide an overview of the different approaches, possible mechanisms, the crops and viruses to which they have been applied, and progress toward commercial applications.


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