Systemic resistance induced in Arabidopsis thaliana by Trichoderma asperellum SKT-1, a microbial pesticide of seedborne diseases of rice

2011 ◽  
Vol 68 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Yohei Yoshioka ◽  
Haruki Ichikawa ◽  
Hushna Ara Naznin ◽  
Atsushi Kogure ◽  
Mitsuro Hyakumachi
Keyword(s):  
Arabidopsis Thaliana ◽  
Systemic Resistance ◽  
Trichoderma Asperellum ◽  
Microbial Pesticide ◽  
Seedborne Diseases

scholarly journals Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1

2013 ◽  
Vol 29 (2) ◽  
pp. 193-200 ◽  
Author(s):  
Mohsen Mohamed Elsharkawy ◽  
Masafumi Shimizu ◽  
Hideki Takahashi ◽  
Kouichi Ozaki ◽  
Mitsuro Hyakumachi
Keyword(s):  
Arabidopsis Thaliana ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Systemic Resistance ◽  
Trichoderma Asperellum

Induction of systemic resistance in turmeric by rhizospheric isolate Trichoderma asperellum against rhizome rot disease

2019 ◽  
Vol 101 (4) ◽  
pp. 965-980 ◽  
Author(s):  
Govindegowda Vinayarani ◽  
Kallahally Nagendra Madhusudhan ◽  
Harishchandra Sripathi Prakash
Keyword(s):  
Systemic Resistance ◽  
Trichoderma Asperellum ◽  
Rot Disease ◽  
Rhizome Rot

scholarly journals Bacillus cereus AR156 Activates Defense Responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana Similarly to flg22

2018 ◽  
Vol 31 (3) ◽  
pp. 311-322 ◽  
Author(s):  
Shune Wang ◽  
Ying Zheng ◽  
Chun Gu ◽  
Chan He ◽  
Mengying Yang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacillus Cereus ◽  
Pseudomonas Syringae ◽  
Transcriptional Profiling ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Callose Deposition ◽  
Plant Growth Promoting

Bacillus cereus AR156 (AR156) is a plant growth–promoting rhizobacterium capable of inducing systemic resistance to Pseudomonas syringae pv. tomato in Arabidopsis thaliana. Here, we show that, when applied to Arabidopsis leaves, AR156 acted similarly to flg22, a typical pathogen-associated molecular pattern (PAMP), in initiating PAMP-triggered immunity (PTI). AR156-elicited PTI responses included phosphorylation of MPK3 and MPK6, induction of the expression of defense-related genes PR1, FRK1, WRKY22, and WRKY29, production of reactive oxygen species, and callose deposition. Pretreatment with AR156 still significantly reduced P. syringae pv. tomato multiplication and disease severity in NahG transgenic plants and mutants sid2-2, jar1, etr1, ein2, npr1, and fls2. This suggests that AR156-induced PTI responses require neither salicylic acid, jasmonic acid, and ethylene signaling nor flagella receptor kinase FLS2, the receptor of flg22. On the other hand, AR156 and flg22 acted in concert to differentially regulate a number of AGO1-bound microRNAs that function to mediate PTI. A full-genome transcriptional profiling analysis indicated that AR156 and flg22 activated similar transcriptional programs, coregulating the expression of 117 genes; their concerted regulation of 16 genes was confirmed by real-time quantitative polymerase chain reaction analysis. These results suggest that AR156 activates basal defense responses to P. syringae pv. tomato in Arabidopsis, similarly to flg22.

scholarly journals Transcript and metabolite analysis of the Trichoderma-induced systemic resistance response to Pseudomonas syringae in Arabidopsis thaliana

Microbiology ◽  
2012 ◽  
Vol 158 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Yariv Brotman ◽  
Jan Lisec ◽  
Michaël Méret ◽  
Ilan Chet ◽  
Lothar Willmitzer ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pseudomonas Syringae ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Metabolite Analysis ◽  
Resistance Response

scholarly journals Induced Systemic Resistance in Arabidopsis thaliana Against Pseudomonas syringae pv. tomato by 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens

2012 ◽  
Vol 102 (4) ◽  
pp. 403-412 ◽  
Author(s):  
David M. Weller ◽  
Dmitri V. Mavrodi ◽  
Johan A. van Pelt ◽  
Corné M. J. Pieterse ◽  
Leendert C. van Loon ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pseudomonas Fluorescens ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Induced Systemic Resistance ◽  
Signal Transduction Pathway ◽  
Systemic Resistance ◽  
Wild Type ◽  
Challenge Inoculation ◽  
Dependent Signal

Pseudomonas fluorescens strains that produce the polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) are among the most effective rhizobacteria that suppress root and crown rots, wilts, and damping-off diseases of a variety of crops, and they play a key role in the natural suppressiveness of some soils to certain soilborne pathogens. Root colonization by 2,4-DAPG-producing P. fluorescens strains Pf-5 (genotype A), Q2-87 (genotype B), Q8r1-96 (genotype D), and HT5-1 (genotype N) produced induced systemic resistance (ISR) in Arabidopsis thaliana accession Col-0 against bacterial speck caused by P. syringae pv. tomato. The ISR-eliciting activity of the four bacterial genotypes was similar, and all genotypes were equivalent in activity to the well-characterized strain P. fluorescens WCS417r. The 2,4-DAPG biosynthetic locus consists of the genes phlHGF and phlACBDE. phlD or phlBC mutants of Q2-87 (2,4-DAPG minus) were significantly reduced in ISR activity, and genetic complementation of the mutants restored ISR activity back to wild-type levels. A phlF regulatory mutant (overproducer of 2,4-DAPG) had ISR activity equivalent to the wild-type Q2-87. Introduction of DAPG into soil at concentrations of 10 to 250 μM 4 days before challenge inoculation induced resistance equivalent to or better than the bacteria. Strain Q2-87 induced resistance on transgenic NahG plants but not on npr1-1, jar1, and etr1 Arabidopsis mutants. These results indicate that the antibiotic 2,4-DAPG is a major determinant of ISR in 2,4-DAPG-producing P. fluorescens, that the genotype of the strain does not affect its ISR activity, and that the activity induced by these bacteria operates through the ethylene- and jasmonic acid-dependent signal transduction pathway.

scholarly journals Induced Systemic Resistance in Arabidopsis thaliana in Response to Root Inoculation with Pseudomonas fluorescens CHA0

2003 ◽  
Vol 16 (10) ◽  
pp. 851-858 ◽  
Author(s):  
Annalisa Iavicoli ◽  
Emmanuel Boutet ◽  
Antony Buchala ◽  
Jean-Pierre Métraux
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Pseudomonas Fluorescens ◽  
Transgenic Line ◽  
Induced Systemic Resistance ◽  
Antibiotic Activity ◽  
Systemic Resistance ◽  
Peronospora Parasitica ◽  
Molecular Processes ◽  
Molecular Determinants

Root inoculation of Arabidopsis thaliana ecotype Columbia with Pseudomonas fluorescens CHA0r partially protected leaves from the oomycete Peronospora parasitica. The molecular determinants of Pseudomonas fluorescens CHA0r for this induced systemic resistance (ISR) were investigated, using mutants derived from strain CHA0: CHA400 (pyoverdine deficient), CHA805 (exoprotease deficient), CHA77 (HCN deficient), CHA660 (pyoluteorin deficient), CHA631 (2,4-diacetylphloroglucinol [DAPG] deficient), and CHA89 (HCN, DAPG- and pyoluteorin deficient). Only mutations interfering with DAPG production led to a significant decrease in ISR to Peronospora parasitica. Thus, DAPG production in Pseudomonas fluorescens is required for the induction of ISR to Peronospora parasitica. DAPG is known for its antibiotic activity; however, our data indicate that one action of DAPG could be due to an effect on the physiology of the plant. DAPG at 10 to 100 μM applied to roots of Arabidopsis mimicked the ISR effect. CHA0r-mediated ISR was also tested in various Arabidopsis mutants and transgenic plants: NahG (transgenic line degrading salicylic acid [SA]), sid2-1 (nonproducing SA), npr1-1 (non-expressing NPR1 protein), jar1-1 (insensitive to jasmonic acid and methyl jasmonic acid), ein2-1 (insensitive to ethylene), etr1-1 (insensitive to ethylene), eir1-1 (insensitive to ethylene in roots), and pad2-1 (phytoalexin deficient). Only jar1-1, eir1-1, and npr1-1 mutants were unable to undergo ISR. Sensitivity to jasmonic acid and functional NPR1 and EIR1 proteins were required for full expression of CHA0r-mediated ISR. The requirements for ISR observed in this study in Peronospora parasitica induced by Pseudomonas fluorescens CHA0r only partially overlap with those published so far for Peronospora parasitica, indicating a great degree of flexibility in the molecular processes leading to ISR.

scholarly journals Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression

2005 ◽  
Vol 18 (6) ◽  
pp. 555-561 ◽  
Author(s):  
Sotirios E. Tjamos ◽  
Emmanouil Flemetakis ◽  
Epaminondas J. Paplomatas ◽  
Panagiotis Katinakis
Keyword(s):  
Arabidopsis Thaliana ◽  
Verticillium Dahliae ◽  
Biocontrol Agent ◽  
Systemic Resistance ◽  
Antagonistic Action ◽  
Arabidopsis Mutants ◽  
Paenibacillus Alvei ◽  
Pathogenesis Related ◽  
Induction Of Resistance ◽  
Related Proteins

The biocontrol bacterium Paenibacillus alvei K165 has the ability to protect Arabidopsis thaliana against Verticillium dahliae. A direct antagonistic action of strain K165 against V. dahliae was ruled out, making it likely that K165-mediated protection results from induced systemic resistance (ISR) in the host. K165-mediated protection was tested in various Arabidopsis mutants and transgenic plants impaired in defense signaling pathways, including NahG (transgenic line degrading salicylic acid [SA]), etr1-1 (insensitive to ethylene), jar1-1 (insensitive to jasmonate), npr1-1 (non-expressing NPR1 protein), pad3-1 (phytoalexin deficient), pad4-1 (phytoalexin deficient), eds5/sid1 (enhanced disease susceptibility), and sid2 (SA-induction deficient). ISR was blocked in blocked in Arabidopsis mutants npr1-1, eds5/sid1, and sid2, indicating that components of the pathway from isochorismate and a functional NPR1 play a crucial role in the K165-mediated ISR. Furthermore, the concomitant activation and increased transient accumulation of the PR-1, PR-2, and PR-5 genes were observed in the treatment in which both the inducing bacterial strain and the challenging pathogen were present in the rhizosphere of the A. thaliana plants.

scholarly journals Function of miR825 and miR825* as Negative Regulators in Bacillus cereus AR156-elicited Systemic Resistance to Botrytis cinerea in Arabidopsis thaliana

2019 ◽  
Vol 20 (20) ◽  
pp. 5032 ◽  
Author(s):  
Pingping Nie ◽  
Chen Chen ◽  
Qian Yin ◽  
Chunhao Jiang ◽  
Jianhua Guo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacillus Cereus ◽  
Botrytis Cinerea ◽  
Target Genes ◽  
Interactive Effect ◽  
Defense Responses ◽  
Northern Blotting ◽  
Systemic Resistance ◽  
Negative Regulators ◽  
Callose Deposition

Small RNAs function to regulate plant defense responses to pathogens. We previously showed that miR825 and miR825* downregulate Bacillus cereus AR156 (AR156)-triggered systemic resistance to Pseudomonassyringae pv. tomato DC3000 in Arabidopsis thaliana (Arabidopsis). Here, Northern blotting revealed that miR825 and miR825* were more strongly downregulated in wild type Arabidopsis Col-0 (Col-0) plants pretreated with AR156 than in nontreated plants upon Botrytis cinerea (B. cinerea) B1301 infection. Furthermore, compared with Col-0, transgenic plants with attenuated miR825 and miR825* expression were more resistant to B. cinerea B1301, yet miR825- and miR825*-overexpressing (OE) plants were more susceptible to the pathogen. With AR156 pretreatment, the transcription of four defense-related genes (PR1, PR2, PR5, and PDF1.2) and cellular defense responses (hydrogen peroxide production and callose deposition) were faster and stronger in miR825 and miR825* knockdown lines but weaker in their OE plants than in Col-0 plants upon pathogen attack. Also, AR156 pretreatment caused stronger phosphorylation of MPK3 and MPK6 and expression of FRK1 and WRKY53 genes upon B. cinerea B1301 inoculation in miR825 and miR825* knockdown plants than in Col-0 plants. Additionally, the assay of agrobacterium-mediated transient co-expression in Nicotiana benthamiana confirmed that AT5G40910, AT5G38850, AT3G04220, and AT5G44940 are target genes of miR825 or miR825*. Compared with Col-0, the target mutant lines showed higher susceptibility to B. cinerea B1301, while still expressing AR156-triggered induced systemic resistance (ISR). The two-way analysis of variance (ANOVA) revealed a significant (P < 0.01) interactive effect of treatment and genotype on the defense responses. Hence, miR825 and miR825*act as negative regulators of AR156-mediated systemic resistance to B. cinerea B1301 in Arabidopsis.

scholarly journals Induced systemic resistance in cucumber and Arabidopsis thaliana by the combination of Trichoderma harzianum Tr6 and Pseudomonas sp. Ps14

2013 ◽  
Vol 65 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Hamidreza Alizadeh ◽  
Keivan Behboudi ◽  
Masoud Ahmadzadeh ◽  
Mohammad Javan-Nikkhah ◽  
Christos Zamioudis ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Trichoderma Harzianum ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Pseudomonas Sp
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