Crystallization and X-ray analysis of a single fab binding domain from protein L ofPeptostreptococcus magnus

1995 ◽  
Vol 23 (4) ◽  
pp. 610-612 ◽  
Author(s):  
Maninder K. Sohi ◽  
Tommy Wan ◽  
Brian J. Sutton ◽  
Tony Atkinson ◽  
Max A. Atkinson ◽  
...  
Keyword(s):  
Binding Domain ◽  
Protein L ◽  
X Ray

Crystallization and preliminary X-ray diffraction studies of mutants of B1 IgG-binding domain of protein L fromPeptostreptococcus magnus

2000 ◽  
Vol 56 (4) ◽  
pp. 506-508 ◽  
Author(s):  
Keyji Johnsen ◽  
Jason W. O'Neill ◽  
David E. Kim ◽  
David Baker ◽  
Kam Y. J. Zhang
Keyword(s):  
Binding Domain ◽  
X Ray Diffraction ◽  
Protein L ◽  
X Ray ◽  
Igg Binding

scholarly journals X-ray Structures of Progesterone Receptor Ligand Binding Domain in Its Agonist State Reveal Differing Mechanisms for Mixed Profiles of 11β-Substituted Steroids

2012 ◽  
Vol 287 (24) ◽  
pp. 20333-20343 ◽  
Author(s):  
Scott J. Lusher ◽  
Hans C. A. Raaijmakers ◽  
Diep Vu-Pham ◽  
Bert Kazemier ◽  
Rolien Bosch ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Ligand Binding ◽  
Binding Domain ◽  
Ligand Binding Domain ◽  
Receptor Ligand ◽  
X Ray

scholarly journals Crystallisation and preliminary X-ray analysis of the receptor-binding domain of human and bovine α 2 -macroglobulin

FEBS Letters ◽  
1995 ◽  
Vol 372 (1) ◽  
pp. 93-95 ◽  
Author(s):  
Klavs Dolmer ◽  
Lasse B Jenner ◽  
Linda Jacobsen ◽  
Gregers R Andersen ◽  
Trine J Koch ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Binding Domain ◽  
Receptor Binding Domain ◽  
X Ray

scholarly journals Rgg protein structure–function and inhibition by cyclic peptide compounds

2015 ◽  
Vol 112 (16) ◽  
pp. 5177-5182 ◽  
Author(s):  
Vijay Parashar ◽  
Chaitanya Aggarwal ◽  
Michael J. Federle ◽  
Matthew B. Neiditch
Keyword(s):  
Dna Binding ◽  
Structure Function ◽  
Cyclosporin A ◽  
Crystal Structures ◽  
Disulfide Bond ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
X Ray ◽  
Peptide Pheromone ◽  
Dimerization Interface

Peptide pheromone cell–cell signaling (quorum sensing) regulates the expression of diverse developmental phenotypes (including virulence) in Firmicutes, which includes common human pathogens, e.g.,Streptococcus pyogenesandStreptococcus pneumoniae. Cytoplasmic transcription factors known as “Rgg proteins” are peptide pheromone receptors ubiquitous in Firmicutes. Here we present X-ray crystal structures of aStreptococcusRgg protein alone and in complex with a tight-binding signaling antagonist, the cyclic undecapeptide cyclosporin A. To our knowledge, these represent the first Rgg protein X-ray crystal structures. Based on the results of extensive structure–function analysis, we reveal the peptide pheromone-binding site and the mechanism by which cyclosporin A inhibits activation of the peptide pheromone receptor. Guided by the Rgg–cyclosporin A complex structure, we predicted that the nonimmunosuppressive cyclosporin A analog valspodar would inhibit Rgg activation. Indeed, we found that, like cyclosporin A, valspodar inhibits peptide pheromone activation of conserved Rgg proteins in medically relevantStreptococcusspecies. Finally, the crystal structures presented here revealed that the Rgg protein DNA-binding domains are covalently linked across their dimerization interface by a disulfide bond formed by a highly conserved cysteine. The DNA-binding domain dimerization interface observed in our structures is essentially identical to the interfaces previously described for other members of the XRE DNA-binding domain family, but the presence of an intermolecular disulfide bond buried in this interface appears to be unique. We hypothesize that this disulfide bond may, under the right conditions, affect Rgg monomer–dimer equilibrium, stabilize Rgg conformation, or serve as a redox-sensitive switch.

Solution NMR Structure and X-ray Absorption Analysis of the C-Terminal Zinc-Binding Domain of the SecA ATPase†

Biochemistry ◽  
2004 ◽  
Vol 43 (29) ◽  
pp. 9361-9371 ◽  
Author(s):  
Brian R. Dempsey ◽  
Mark Wrona ◽  
Jana M. Moulin ◽  
Gregory B. Gloor ◽  
Farideh Jalilehvand ◽  
...  
Keyword(s):  
Binding Domain ◽  
Nmr Structure ◽  
Zinc Binding ◽  
Solution Nmr ◽  
Absorption Analysis ◽  
X Ray ◽  
Zinc Binding Domain ◽  
X Ray Absorption
Sign in / Sign up

Export Citation Format

Share Document