Translational regulation of ribosomal protein S15 drives characteristic patterns of protein-mRNA epistasis

2018 ◽  
Vol 86 (8) ◽  
pp. 827-832 ◽  
Author(s):  
Saurav Mallik ◽  
Sudipto Basu ◽  
Suman Hait ◽  
Sudip Kundu
Keyword(s):  
Ribosomal Protein ◽  
Translational Regulation ◽  
Ribosomal Protein S15

scholarly journals Molecular Mimicry in Translational Regulation: The Case of Ribosomal Protein S15

RNA Biology ◽  
2004 ◽  
Vol 1 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Chantal Ehresmann ◽  
Bernard Ehresmann ◽  
Eric Ennifar ◽  
Philippe Dumas ◽  
Maria Garber ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Translational Regulation ◽  
Molecular Mimicry ◽  
Ribosomal Protein S15

scholarly journals The 5' untranslated region of mRNA for ribosomal protein S19 is involved in its translational regulation during Xenopus development.

1990 ◽  
Vol 10 (2) ◽  
pp. 816-822 ◽  
Author(s):  
P Mariottini ◽  
F Amaldi
Keyword(s):  
Ribosomal Protein ◽  
Untranslated Region ◽  
Ribosomal Proteins ◽  
Translational Regulation ◽  
Xenopus Development ◽  
Untranslated Sequence ◽  
Gene Coding ◽  
Ribosomal Protein S19 ◽  
Fused Gene

During Xenopus development, the synthesis of ribosomal proteins is regulated at the translational level. To identify the region of the ribosomal protein mRNAs responsible for their typical translational behavior, we constructed a fused gene in which the upstream sequences (promoter) and the 5' untranslated sequence (first exon) of the gene coding for Xenopus ribosomal protein S19 were joined to the coding portion of the procaryotic chloramphenicol acetyltransferase (CAT) gene deleted of its own 5' untranslated region. This fused gene was introduced in vivo by microinjection into Xenopus fertilized eggs, and its activity was monitored during embryogenesis. By analyzing the pattern of appearance of CAT activity and the distribution of the S19-CAT mRNA between polysomes and messenger ribonucleoproteins, it was concluded that the 35-nucleotide-long 5' untranslated region of the S19 mRNA is able to confer to the fused S19-CAT mRNA the translational behavior typical of ribosomal proteins during Xenopus embryo development.

Translational regulation of ribosomal protein synthesis during xenopus development

1989 ◽  
Vol 27 ◽  
pp. 219
Author(s):  
B. Cardinali ◽  
C. Bagni ◽  
F. Amaldi ◽  
N. Campioni ◽  
P. Mariottini ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Protein ◽  
Translational Regulation ◽  
Xenopus Development ◽  
Ribosomal Protein Synthesis

Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO)

1984 ◽  
Vol 197 (2) ◽  
pp. 225-229 ◽  
Author(s):  
Renkichi Takata ◽  
Tsunehiro Mukai ◽  
Michiko Aoyagi ◽  
Katsuji Hori
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15

Ribosomal Protein S15 from Thermus Thermophilus. Cloning, Sequencing, Overexpression of the Gene and RNA-Binding Properties of the Protein

1997 ◽  
Vol 246 (2) ◽  
pp. 291-300 ◽  
Author(s):  
Alexander Serganov ◽  
Alexey Rak ◽  
Maria Garber ◽  
Joseph Reinbolt ◽  
Bernard Ehresmann ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Rna Binding ◽  
Thermus Thermophilus ◽  
Binding Properties ◽  
Ribosomal Protein S15

scholarly journals Dedicated chaperones coordinate co-translational regulation of ribosomal protein production with ribosome assembly to preserve proteostasis

2021 ◽  
Author(s):  
Benjamin Pillet ◽  
Alfonso Méndez-Godoy ◽  
Guillaume Murat ◽  
Sébastien Favre ◽  
Michael Stumpe ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Translational Regulation ◽  
De Novo ◽  
Spatial Separation ◽  
Ribosome Assembly ◽  
Mrna Levels ◽  
Ribosomal Assembly ◽  
Surplus Production ◽  
Regulatory Machinery

AbstractThe biogenesis of eukaryotic ribosomes involves the ordered assembly of around 80 ribosomal proteins. Supplying equimolar amounts of assembly-competent ribosomal proteins is complicated by their aggregation propensity and the spatial separation of their location of synthesis and pre-ribosome incorporation. Recent evidence has highlighted that dedicated chaperones protect individual, unassembled ribosomal proteins on their path to the pre-ribosomal assembly site. Here, we show that the co-translational recognition of Rpl3 and Rpl4 by their respective dedicated chaperone, Rrb1 or Acl4, prevents the degradation of the encoding RPL3 and RPL4 mRNAs in the yeast Saccharomyces cerevisiae. In both cases, negative regulation of mRNA levels occurs when the availability of the dedicated chaperone is limited and the nascent ribosomal protein is instead accessible to a regulatory machinery consisting of the nascent-polypeptide associated complex and the Caf130-associated Ccr4-Not complex. Notably, deregulated expression of Rpl3 and Rpl4 leads to their massive aggregation and a perturbation of overall proteostasis in cells lacking the E3 ubiquitin ligase Tom1. Taken together, we have uncovered an unprecedented regulatory mechanism that adjusts the de novo synthesis of Rpl3 and Rpl4 to their actual consumption during ribosome assembly and, thereby, protects cells from the potentially detrimental effects of their surplus production.

scholarly journals Knockout of Pi21 by CRISPR/Cas9 and iTRAQ-Based Proteomic Analysis of Mutants Revealed New Insights into M. oryzae Resistance in Elite Rice Line

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 735 ◽  
Author(s):  
Gul Nawaz ◽  
Babar Usman ◽  
Haowen Peng ◽  
Neng Zhao ◽  
Ruizhi Yuan ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Proteomic Analysis ◽  
Defense Mechanism ◽  
Agronomic Traits ◽  
Blast Resistance ◽  
Metabolic Process ◽  
Targeted Mutagenesis ◽  
Fundamental Value ◽  
Ribosomal Protein S15 ◽  
Mutant Lines

Rice blast (Magnaporthe oryzae) is a devastating disease affecting rice production globally. The development of cultivars with host resistance has been proved to be the best strategy for disease management. Several rice-resistance genes (R) have been recognized which induce resistance to blast in rice but R gene-mediated mechanisms resulting in defense response still need to be elucidated. Here, mutant lines generated through CRISPR/Cas9 based targeted mutagenesis to investigate the role of Pi21 against blast resistance and 17 mutant plants were obtained in T0 generation with the mutation rate of 66% including 26% bi-allelic, 22% homozygous, 12% heterozygous, and 3% chimeric and 17 T-DNA-free lines in T1 generation. The homozygous mutant lines revealed enhanced resistance to blast without affecting the major agronomic traits. Furthermore, comparative proteome profiling was adopted to study the succeeding proteomic regulations, using iTRAQ-based proteomic analysis. We identified 372 DEPs, among them 149 up and 223 were down-regulated, respectively. GO analysis revealed that the proteins related to response to stimulus, photosynthesis, carbohydrate metabolic process, and small molecule metabolic process were up-regulated. The most of DEPs were involved in metabolic, ribosomal, secondary metabolites biosynthesis, and carbon metabolism pathways. 40S ribosomal protein S15 (P31674), 50S ribosomal protein L4, L5, L6 (Q10NM5, Q9ZST0, Q10L93), 30S ribosomal protein S5, S9 (Q6YU81, Q850W6, Q9XJ28), and succinate dehydrogenase (Q9S827) were hub-proteins. The expression level of genes related to defense mechanism, involved in signaling pathways of jasmonic acid (JA), salicylic acid (SA), and ethylene metabolisms were up-regulated in mutant line after the inoculation of the physiological races of M. oryzae as compared to WT. Our results revealed the fundamental value of genome editing and expand knowledge about fungal infection avoidance in rice.

scholarly journals A Ribosomal Protein Is Required for Translational Regulation ofGCN4mRNA

1998 ◽  
Vol 273 (49) ◽  
pp. 32870-32877 ◽  
Author(s):  
Peter P. Mueller ◽  
Patrick Grueter ◽  
Alan G. Hinnebusch ◽  
Hans Trachsel
Keyword(s):  
Ribosomal Protein ◽  
Translational Regulation

Translational autocontrol of the Escherichia coli ribosomal protein S15

1990 ◽  
Vol 211 (2) ◽  
pp. 407-414 ◽  
Author(s):  
Claude Portier ◽  
Liliane Dondon ◽  
Marianne Grunberg-Manago
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15
Sign in / Sign up

Export Citation Format

Share Document