scholarly journals Knockout of Pi21 by CRISPR/Cas9 and iTRAQ-Based Proteomic Analysis of Mutants Revealed New Insights into M. oryzae Resistance in Elite Rice Line

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 735 ◽  
Author(s):  
Gul Nawaz ◽  
Babar Usman ◽  
Haowen Peng ◽  
Neng Zhao ◽  
Ruizhi Yuan ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Proteomic Analysis ◽  
Defense Mechanism ◽  
Agronomic Traits ◽  
Blast Resistance ◽  
Metabolic Process ◽  
Targeted Mutagenesis ◽  
Fundamental Value ◽  
Ribosomal Protein S15 ◽  
Mutant Lines

Rice blast (Magnaporthe oryzae) is a devastating disease affecting rice production globally. The development of cultivars with host resistance has been proved to be the best strategy for disease management. Several rice-resistance genes (R) have been recognized which induce resistance to blast in rice but R gene-mediated mechanisms resulting in defense response still need to be elucidated. Here, mutant lines generated through CRISPR/Cas9 based targeted mutagenesis to investigate the role of Pi21 against blast resistance and 17 mutant plants were obtained in T0 generation with the mutation rate of 66% including 26% bi-allelic, 22% homozygous, 12% heterozygous, and 3% chimeric and 17 T-DNA-free lines in T1 generation. The homozygous mutant lines revealed enhanced resistance to blast without affecting the major agronomic traits. Furthermore, comparative proteome profiling was adopted to study the succeeding proteomic regulations, using iTRAQ-based proteomic analysis. We identified 372 DEPs, among them 149 up and 223 were down-regulated, respectively. GO analysis revealed that the proteins related to response to stimulus, photosynthesis, carbohydrate metabolic process, and small molecule metabolic process were up-regulated. The most of DEPs were involved in metabolic, ribosomal, secondary metabolites biosynthesis, and carbon metabolism pathways. 40S ribosomal protein S15 (P31674), 50S ribosomal protein L4, L5, L6 (Q10NM5, Q9ZST0, Q10L93), 30S ribosomal protein S5, S9 (Q6YU81, Q850W6, Q9XJ28), and succinate dehydrogenase (Q9S827) were hub-proteins. The expression level of genes related to defense mechanism, involved in signaling pathways of jasmonic acid (JA), salicylic acid (SA), and ethylene metabolisms were up-regulated in mutant line after the inoculation of the physiological races of M. oryzae as compared to WT. Our results revealed the fundamental value of genome editing and expand knowledge about fungal infection avoidance in rice.

Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO)

1984 ◽  
Vol 197 (2) ◽  
pp. 225-229 ◽  
Author(s):  
Renkichi Takata ◽  
Tsunehiro Mukai ◽  
Michiko Aoyagi ◽  
Katsuji Hori
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15

Ribosomal Protein S15 from Thermus Thermophilus. Cloning, Sequencing, Overexpression of the Gene and RNA-Binding Properties of the Protein

1997 ◽  
Vol 246 (2) ◽  
pp. 291-300 ◽  
Author(s):  
Alexander Serganov ◽  
Alexey Rak ◽  
Maria Garber ◽  
Joseph Reinbolt ◽  
Bernard Ehresmann ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Rna Binding ◽  
Thermus Thermophilus ◽  
Binding Properties ◽  
Ribosomal Protein S15

Production of High Amylose and Resistant Starch Rice Through Targeted Mutagenesis of Starch Branching Enzyme Iib by Crispr/cas9

2021 ◽  
Author(s):  
Hsi-Chao Wang ◽  
Yu-Chia Hsu ◽  
Yong-Pei Wu ◽  
Su-Ying Yeh ◽  
Maurice S. B. Ku
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Resistant Starch ◽  
Targeted Mutagenesis ◽  
Rice Starch ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Homozygous Mutant ◽  
Mutant Lines ◽  
High Amylose

Abstract Rice is the staple food for half of the world’s population. Starch accounts for 80-90% of the total mass of rice seeds, and rice starch is low in resistant starch (RS) with a high glycemic index (GI). RS has gained important since it is beneficial in preventing various diseases. Starch branching enzyme IIb (SBEIIb) plays a key role in the amylopectin synthesis pathway in the endosperm of cereals. Down-regulation of SBEIIb in several important crops has led to high amylose, high RS and low GI starch. In this study, we mutated OsSBEIIb in the japonica rice cultivar TNG82 through CRISPR/Cas9 and investigated the molecular and physicochemical modifications in OsSBEIIb mutant lines, e.g., gene expression, enzyme activity, apparent amylose content (AAC), RS and GI. As expected, the levels of modification in these starch related traits in heterozygous mutant lines were about half as those of homozygous mutant lines. Gene expression and enzyme activity of OsSBEIIb were down-regulated significantly while AAC and RS contents increased progressively from 17.4% and 0.5% in WT, respectively, to as high as 25.0% and 7.5% in heterozygous mutant lines and 36.0% and 12.0% in homozygous mutant lines. Consequently, with increased RS and decreased rate of reducing sugar production, GI progressively decreased in heterozygous and homozygous mutant rice endosperms by 11% and 28%, respectively. Our results demonstrate that it has huge potential for precise and efficient generation of high RS and low GI rice through CRISPR/Cas9 to provide a more suitable source of starch for type II diabetes.

scholarly journals Coevolutionary Dynamics of Rice Blast Resistance Gene Pi-ta and Magnaporthe oryzae Avirulence Gene AVR-Pita 1

2016 ◽  
Vol 106 (7) ◽  
pp. 676-683 ◽  
Author(s):  
Yulin Jia ◽  
Erxun Zhou ◽  
Seonghee Lee ◽  
Tracy Bianco
Keyword(s):  
Magnaporthe Oryzae ◽  
Marker Assisted Selection ◽  
Defense Mechanism ◽  
Allelic Variation ◽  
Blast Resistance ◽  
Quantitative Resistance ◽  
Association Studies ◽  
The United States ◽  
Avirulence Gene ◽  
Genome Wide Association Studies

The Pi-ta gene in rice is effective in preventing infections by Magnaporthe oryzae strains that contain the corresponding avirulence gene, AVR-Pita1. Diverse haplotypes of AVR-Pita1 have been identified from isolates of M. oryzae from rice production areas in the United States and worldwide. DNA sequencing and mapping studies have revealed that AVR-Pita1 is highly unstable, while expression analysis and quantitative resistance loci mapping of the Pi-ta locus revealed complex evolutionary mechanisms of Pi-ta-mediated resistance. Among these studies, several Pi-ta transcripts were identified, most of which are probably derived from alternative splicing and exon skipping, which could produce functional resistance proteins that support a new concept of coevolution of Pi-ta and AVR-Pita1. User-friendly DNA markers for Pi-ta have been developed to support marker-assisted selection, and development of new rice varieties with the Pi-ta markers. Genome-wide association studies revealed a link between Pi-ta-mediated resistance and yield components suggesting that rice has evolved a complicated defense mechanism against the blast fungus. In this review, we detail the current understanding of Pi-ta allelic variation, its linkage with rice productivity, AVR-Pita allelic variation, and the coevolution of Pi-ta and AVR-Pita in Oryza species and M. oryzae populations, respectively. We also review the genetic and molecular basis of Pi-ta and AVR-Pita interaction, and its value in marker-assisted selection and engineering resistance.

Assessment of genetic diversity in Italian rice germplasm related to agronomic traits and blast resistance (Magnaporthe oryzae)

2010 ◽  
Vol 27 (2) ◽  
pp. 233-246 ◽  
Author(s):  
Odile Faivre-Rampant ◽  
Gianluca Bruschi ◽  
Pamela Abbruscato ◽  
Stefano Cavigiolo ◽  
Anna Maria Picco ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Magnaporthe Oryzae ◽  
Agronomic Traits ◽  
Blast Resistance ◽  
Rice Germplasm

Translational autocontrol of the Escherichia coli ribosomal protein S15

1990 ◽  
Vol 211 (2) ◽  
pp. 407-414 ◽  
Author(s):  
Claude Portier ◽  
Liliane Dondon ◽  
Marianne Grunberg-Manago
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15

The primary structure of rat rig/ribosomal protein S15 gene

1992 ◽  
Vol 1132 (2) ◽  
pp. 228-230 ◽  
Author(s):  
Shin Takasawa ◽  
Akira Tohgo ◽  
Michiaki Unno ◽  
Kiyoto Shiga ◽  
Hideto Yonekura ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Primary Structure ◽  
Ribosomal Protein S15

Sequence of the chicken rig gene encoding ribosomal protein S15

Gene ◽  
1991 ◽  
Vol 108 (2) ◽  
pp. 313-314 ◽  
Author(s):  
Sugawara Akira ◽  
Shiga Kiyoto ◽  
Takasawa Shin ◽  
Yonekura Hideto ◽  
Yamamoto Hiroshi ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Gene Encoding ◽  
Ribosomal Protein S15
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