Insight into the stability of the hydrophobic binding proteins ofescherichia coli: Assessing the proteins for use as biosensors

2003 ◽  
Vol 53 (2) ◽  
pp. 273-281 ◽  
Author(s):  
Branka Salopek-Sondi ◽  
Matthew C. Skeels ◽  
Derrick Swartz ◽  
Linda A. Luck
Keyword(s):  
Binding Proteins ◽  
Ofescherichia Coli ◽  
Hydrophobic Binding ◽  
The Stability ◽  
Insight Into

scholarly journals Bioinspired organometallic chemistry

2002 ◽  
Vol 74 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Lanny S. Liebeskind ◽  
Jiri Srogl ◽  
Cecile Savarin ◽  
Concepcion Polanco
Keyword(s):  
Organometallic Chemistry ◽  
Refractory Metal ◽  
Redox Active ◽  
The Stability ◽  
New Procedures ◽  
Sulfur Containing ◽  
Insight Into

Given the stability of the bond between a mercaptide ligand and various redox-active metals, it is of interest that Nature has evolved significant metalloenzymatic processes that involve key interactions of sulfur-containing functionalities with metals such as Ni, Co, Cu, and Fe. From a chemical perspective, it is striking that these metals can function as robust biocatalysts in vivo, even though they are often "poisoned" as catalysts in vitro through formation of refractory metal thiolates. Insight into the nature of this chemical discrepancy is under study in order to open new procedures in synthetic organic and organometallic chemistry.

scholarly journals On the Trigonometric Descriptions of Colors

2016 ◽  
Vol 8 (3) ◽  
pp. 5
Author(s):  
Jirí Stavek
Keyword(s):  
Experimental Data ◽  
Transverse Energy ◽  
Gravitational Constant ◽  
Isaac Newton ◽  
Type Ia ◽  
New Interpretation ◽  
The Stability ◽  
Mass Evaporation ◽  
Supernovae Type Ia ◽  
Insight Into

<p class="1Body">An attempt is presented for the description of the spectral colors using the standard trigonometric tools in order to extract more information about photons. We have arranged the spectral colors on an arc of the circle with the radius R = 1 and the central angle θ = π/3 when we have defined cos (θ) = λ<sub>380</sub>/λ<sub>760</sub> = 0.5. Several trigonometric operations were applied in order to find the gravity centers for the scotopic, photopic, and mesopic visions. The concept of the center of gravity of colors introduced Isaac Newton. We have postulated properties of the long-lived photons with the new interpretation of the Hubble (Zwicky-Nernst) constant H<sub>0</sub> = 2.748… * 10<sup>-18</sup> kg kg<sup>-1 </sup>s<sup>-1</sup>, the specific mass evaporation rate (SMER) of gravitons from the source mass. The stability of international prototypes of kilogram has been regularly checked. We predict that those standard kilograms due to the evaporation of gravitons lost 8.67 μg kg<sup>-1</sup> century<sup>-1</sup>. The energy of long-lived photons was trigonometrically decomposed into three parts that could be experimentally tested: longitudinal energy, transverse energy and energy of evaporated gravitons. We tested the properties of the long-lived photons with the experimental data published for the best available standard candles: supernovae Type Ia. There was found a surprising match of those experimental data with the model of the long-lived photons. Finally, we have proposed a possible decomposition of the big G (Newtonian gravitational constant) and the small kappa κ (Einsteinian gravitational constant) in order to get a new insight into the mysterious gravitational force and/or the curvature concept.</p>

scholarly journals Influence of additives on suspension structure

2021 ◽  
Vol 10 (1) ◽  
pp. 37-40
Author(s):  
Vesna Savić ◽  
Milica Martinović ◽  
Ivana Nešić ◽  
Jelena Živković ◽  
Ivana Gajić
Keyword(s):  
Zinc Oxide ◽  
The Other ◽  
Stability Tests ◽  
Polysorbate 20 ◽  
The Stability ◽  
Carbomer Gel ◽  
Suspension Structure ◽  
Insight Into ◽  
Study Offer ◽  
Measured Hardness

The aim of this study was to compare the stability and texture of three zinc oxide suspensions with different additives. Suspension 1 was made as official magistral formulation Suspensio album 7.5% from Formulae magistrales 2008. Suspension 2 was prepared when 1% carbomer gel was added to suspension 1 and suspension 3 was prepared when polysorbate 20 was added to suspension 2. After stability tests, texture analysis was performed on all suspensions. Following parameters were measured: hardness cycle 1, hardness cycle 2, cohesiveness, adhesiveness, resilience and springiness. The study showed that suspension 3 had the lowest value of hardness, and therefore the best spreadability. Also, suspension 3 was the least sticky of all three, since it was characterized with the lowest adhesiveness. Further, suspension 3 was the most cohesive and is predicted to withstand the stress during packing and use longer than others. On the other hand, the highest values of resilience and springiness were detected for suspension 1, while the lowest was related to suspension 2. Therefore, the best textural characteristics were assigned to suspension 3. This result is in accordance with the results of performed stability tests. The results of our study offer insight into potential improvements of the current magistral formulation Suspensio album 7.5%.

scholarly journals Een geconstrueerd denken

1970 ◽  
Vol 61 ◽  
pp. 269-292
Author(s):  
Rajesh Heynickx
Keyword(s):  
Main Argument ◽  
Cultural Project ◽  
The Stability ◽  
Insight Into ◽  
The Way

In this article it is demonstrated that an analysis of how building metaphorswere used in the Flemish Catholic discourse of the interwar years can offermore insight into the way a community of believers tries to establish a culturalcohesiveness. The main argument is that in a period of deep transformations,building metaphors could become "instruments" for Catholics whowanted to defend and promote a traditional dimension of their religion.Building metaphors allowed Catholics to stress the stability of their own ideology(the fundaments) and to formulate their own cultural project (buildingplan). By analysing such strategic use of building metaphors in artistic andphilosophical discourses, it can become possible to shed more light on the roleneo-thomism, a main philosophical current in interwar Flanders, played inartistic debates and more specific in discussions on the modernisation of religiousart.

scholarly journals Molecular Oxygen Lignin Depolymerization: An Insight into the Stability of Phenolic Monomers

ChemSusChem ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4743-4758
Author(s):  
Yannick Mathieu ◽  
Juan D. Vidal ◽  
Lourdes Arribas Martínez ◽  
Nerea Abad Fernández ◽  
Sara Iborra ◽  
...  
Keyword(s):  
Molecular Oxygen ◽  
The Stability ◽  
Lignin Depolymerization ◽  
Insight Into

scholarly journals Control of Cytokine mRNA Expression by RNA-binding Proteins and microRNAs

2012 ◽  
Vol 91 (7) ◽  
pp. 651-658 ◽  
Author(s):  
V. Palanisamy ◽  
A. Jakymiw ◽  
E.A. Van Tubergen ◽  
N.J. D’Silva ◽  
K.L. Kirkwood
Keyword(s):  
Cancer Progression ◽  
Mrna Stability ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Periodontal Diseases ◽  
Pharyngeal Cancer ◽  
Cytokine Mrna ◽  
Mediators Of Inflammation ◽  
The Stability

Cytokines are critical mediators of inflammation and host defenses. Regulation of cytokines can occur at various stages of gene expression, including transcription, mRNA export, and post- transcriptional and translational levels. Among these modes of regulation, post-transcriptional regulation has been shown to play a vital role in controlling the expression of cytokines by modulating mRNA stability. The stability of cytokine mRNAs, including TNFα, IL-6, and IL-8, has been reported to be altered by the presence of AU-rich elements (AREs) located in the 3′-untranslated regions (3′UTRs) of the mRNAs. Numerous RNA-binding proteins and microRNAs bind to these 3′UTRs to regulate the stability and/or translation of the mRNAs. Thus, this paper describes the cooperative function between RNA-binding proteins and miRNAs and how they regulate AU-rich elements containing cytokine mRNA stability/degradation and translation. These mRNA control mechanisms can potentially influence inflammation as it relates to oral biology, including periodontal diseases and oral pharyngeal cancer progression.

Role of sterics in phosphine-ligated gold clusters

2019 ◽  
Vol 21 (4) ◽  
pp. 1689-1699 ◽  
Author(s):  
Katherine A. Parrish ◽  
Mary King ◽  
Marshall R. Ligare ◽  
Grant E. Johnson ◽  
Heriberto Hernández
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Ligand Exchange ◽  
Gold Clusters ◽  
Solution Phase ◽  
Ionization Mass ◽  
Exchange Reactions ◽  
The Stability ◽  
Insight Into

This study examined the solution-phase exchange reactions of triphenylphosphine (PPh3) ligands on Au8L72+ (L = PPh3) gold clusters with three different tolyl ligands using electrospray ionization mass spectrometry to provide insight into how steric differences in the phosphines influence the extent of ligand exchange and the stability of the resulting mixed-phosphine clusters.

scholarly journals Unraveling the Impact of Cysteine-to-Serine Mutations on the Structural and Functional Properties of Cu(I)-Binding Proteins

2019 ◽  
Vol 20 (14) ◽  
pp. 3462 ◽  
Author(s):  
Pavlin ◽  
Qasem ◽  
Sameach ◽  
Gevorkyan-Airapetov ◽  
Ritacco ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Cell Function ◽  
Working Mechanism ◽  
Essential Requirement ◽  
Aggregation Propensity ◽  
Structural And Functional Properties ◽  
Stability Structure ◽  
The Stability ◽  
The Impact ◽  
Partner Proteins

Appropriate maintenance of Cu(I) homeostasis is an essential requirement for proper cell function because its misregulation induces the onset of major human diseases and mortality. For this reason, several research efforts have been devoted to dissecting the inner working mechanism of Cu(I)-binding proteins and transporters. A commonly adopted strategy relies on mutations of cysteine residues, for which Cu(I) has an exquisite complementarity, to serines. Nevertheless, in spite of the similarity between these two amino acids, the structural and functional impact of serine mutations on Cu(I)-binding biomolecules remains unclear. Here, we applied various biochemical and biophysical methods, together with all-atom simulations, to investigate the effect of these mutations on the stability, structure, and aggregation propensity of Cu(I)-binding proteins, as well as their interaction with specific partner proteins. Among Cu(I)-binding biomolecules, we focused on the eukaryotic Atox1-ATP7B system, and the prokaryotic CueR metalloregulator. Our results reveal that proteins containing cysteine-to-serine mutations can still bind Cu(I) ions; however, this alters their stability and aggregation propensity. These results contribute to deciphering the critical biological principles underlying the regulatory mechanism of the in-cell Cu(I) concentration, and provide a basis for interpreting future studies that will take advantage of cysteine-to-serine mutations in Cu(I)-binding systems.

scholarly journals Interaction between Polycomb and SSX Proteins in Pericentromeric Heterochromatin Function and Its Implication in Cancer

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 226
Author(s):  
Simone Johansen ◽  
Morten Frier Gjerstorff
Keyword(s):  
Immune Deficiency ◽  
Tumor Biology ◽  
Pericentromeric Heterochromatin ◽  
Control Mechanisms ◽  
Malignant Cells ◽  
Polycomb Proteins ◽  
The Stability ◽  
Polycomb Bodies ◽  
Insight Into ◽  
Cancer Testis

The stability of pericentromeric heterochromatin is maintained by repressive epigenetic control mechanisms, and failure to maintain this stability may cause severe diseases such as immune deficiency and cancer. Thus, deeper insight into the epigenetic regulation and deregulation of pericentromeric heterochromatin is of high priority. We and others have recently demonstrated that pericentromeric heterochromatin domains are often epigenetically reprogrammed by Polycomb proteins in premalignant and malignant cells to form large subnuclear structures known as Polycomb bodies. This may affect the regulation and stability of pericentromeric heterochromatin domains and/or the distribution of Polycomb factors to support tumorigeneses. Importantly, Polycomb bodies in cancer cells may be targeted by the cancer/testis-related SSX proteins to cause derepression and genomic instability of pericentromeric heterochromatin. This review will discuss the interplay between SSX and Polycomb factors in the repression and stability of pericentromeric heterochromatin and its possible implications for tumor biology.

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