Primary culture of epithelial cells derived from the rat ventral prostate: Formation of three-dimensional acinus-like structure in collagen gel

The Prostate ◽  
1987 ◽  
Vol 10 (2) ◽  
pp. 153-161 ◽  
Author(s):  
H. Kawamura ◽  
I. Ichihara
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

Ca2+-activated Cl? channel currents in rat ventral prostate epithelial cells

The Prostate ◽  
2003 ◽  
Vol 55 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Sung Joon Kim ◽  
Sun Young Shin ◽  
Ji Eun Lee ◽  
Jun Hee Kim ◽  
Dae-Yong Uhm
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
Cl Channel ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Channel Currents

Differences in growth requirements between epithelial and stromal cells derived from rat ventral prostate in serum-free primary culture

The Prostate ◽  
1990 ◽  
Vol 17 (3) ◽  
pp. 207-218 ◽  
Author(s):  
Shigeo Taketa ◽  
Nozomu Nishi ◽  
Hirotoshi Takasuga ◽  
Takuya Okutani ◽  
Ikumasa Takenaka ◽  
...  
Keyword(s):  
Primary Culture ◽  
Stromal Cells ◽  
Ventral Prostate ◽  
Serum Free ◽  
Growth Requirements ◽  
Rat Ventral Prostate

K+ channel currents in rat ventral prostate epithelial cells

The Prostate ◽  
2002 ◽  
Vol 51 (3) ◽  
pp. 201-210 ◽  
Author(s):  
Jun Hee Kim ◽  
Eun-Kyung Hong ◽  
Hee Sook Choi ◽  
Seung-Joon Oh ◽  
Kwang Myung Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
K Channel ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Channel Currents

scholarly journals Androgens Regulate the Immune/Inflammatory Response and Cell Survival Pathways in Rat Ventral Prostate Epithelial Cells

Endocrinology ◽  
2006 ◽  
Vol 147 (1) ◽  
pp. 257-271 ◽  
Author(s):  
A. J. Asirvatham ◽  
M. Schmidt ◽  
B. Gao ◽  
J. Chaudhary
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Inflammatory Response ◽  
Immediate Early Genes ◽  
Immediate Early ◽  
Ventral Prostate ◽  
Cell Renewal ◽  
Early Genes ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate

A major hurdle in understanding the role of androgens is the heterogeneity of androgen receptor (AR) expression in the prostate. Because the majority of prostate cancer arises from the AR-positive secretory luminal epithelial cells, identifying the androgen-mediated pathways in the prostate epithelium is of great significance to understanding their role in prostate pathogenesis. To meet this objective, the current study was designed to identify immediate-early genes expressed in response to the synthetic androgen R1881 in cultured rat ventral prostate epithelial cells. Rat ventral prostate epithelial cells, purified from 20-d-old rats, were cultured, and the presence of AR and the response to androgen were established. The cells were then treated with R1881 for 2 and 12 h to capture immediate-early genes in an Affymetrix-based gene chip platform. A total of 66 nonredundant genes were identified that were responsive to R1881. The functional androgen response elements were identified in the proximal promoter to determine possible molecular mechanism. Cluster analysis identified five distinct signatures of R1881-induced genes. Pathway analysis suggested that R1881 primarily influences cell proliferation/differentiation and inflammatory/immune response pathways. Androgens appear to regulate cell renewal by regulating differentiation, cell proliferation, and apoptosis. Two mutually exclusive inflammatory response pathways were observed. The interferon pathway was up-regulated, and the ILs were down-regulated. The data identified novel androgen-regulated genes (e.g. Id1, Id3, IL-6, IGF-binding protein-2 and -3, and JunB). The loss of androgen regulation of these genes can have important consequences for cellular transformation and transition to androgen-independent growth and survival.

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