Identification and characterization of PLZF as a prostatic androgen-responsive gene

The Prostate ◽  
2004 ◽  
Vol 59 (4) ◽  
pp. 426-435 ◽  
Author(s):  
Feng Jiang ◽  
Zhou Wang
Keyword(s):  
Responsive Gene ◽  
Identification And Characterization

scholarly journals Identification and characterization of a novel multi-stress responsive gene in Arabidopsis

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244030
Author(s):  
Faiza Tawab ◽  
Iqbal Munir ◽  
Zeeshan Nasim ◽  
Mohammad Sayyar Khan ◽  
Saleha Tawab ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Survival Rates ◽  
Crop Productivity ◽  
Cell Wall Protein ◽  
Transcriptome Data ◽  
Gus Assay ◽  
Transgenic Lines ◽  
Responsive Gene ◽  
Identification And Characterization

Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.

scholarly journals Identification and Characterization of an Androgen-Responsive Gene Encoding an Aci-Reductone Dioxygenase-Like Protein in the Rat Prostate

Endocrinology ◽  
2004 ◽  
Vol 145 (4) ◽  
pp. 1933-1942 ◽  
Author(s):  
Shane Oram ◽  
Feng Jiang ◽  
Xiaoyan Cai ◽  
Riffat Haleem ◽  
Zehra Dincer ◽  
...  
Keyword(s):  
Gene Encoding ◽  
Responsive Gene ◽  
Rat Prostate ◽  
Identification And Characterization

Identification and characterization of estrogen-responsive gene products in the liver of rainbow trout

1983 ◽  
Vol 61 (7) ◽  
pp. 802-810 ◽  
Author(s):  
Thomas T. Chen
Keyword(s):  
Rainbow Trout ◽  
Egg Laying ◽  
Control Fish ◽  
Estradiol Treatment ◽  
Protease Digestion ◽  
Treated Fish ◽  
Estrogen Responsive Gene ◽  
Responsive Gene ◽  
Identification And Characterization

Injection of male rainbow trout with estradiol induced the production of a major serum protein, vitellogenin (VG), of molecular weight (MW) 170 000. This protein is immunoprecipitable by an antiserum raised against lipovitellin from the eggs. Labelling by incubation of liver cubes of estradiol-treated fish with [35S]methionine and analysis of immunospecific products showed incorporation into two polypeptides of MW 170 000 and 160 000. The peptide patterns generated from these two polypeptides by Staphylococcus aureus V8 protease digestion were identical. Cytoplasmic poly(A)+ RNA of estradiol-treated male liver directs the synthesis of a polypeptide of 160 000 daltons in the reticulocyte cell-free protein-synthesizing system or in Xenopus oocytes. This polypeptide is chemically, immunologically, and electrophoretically identical to the authentic VG polypeptide of 160 000 daltons. Changes in mRNA populations in the liver of male fish following estradiol treatment were detected on gels and by hybridization. On methylmercuric hydroxide gels, a polyadenylated RNA species of about 6300 nucleotides was found in estradiol-treated fish which was absent from the control fish. Data from R0t analysis revealed the presence of at least two mRNA sequences of the high frequency class in the RNA of hormone-induced fish and their sizes were determined by Northern blot analysis to be 6300 and 1800 nucleotides, respectively. These observations are consistent with the induction of vitellogenins and at least another estrogen-responsive gene product by estradiol as reported in other egg-laying vertebrates.

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