Identification and characterization of estrogen-responsive gene products in the liver of rainbow trout

1983 ◽  
Vol 61 (7) ◽  
pp. 802-810 ◽  
Author(s):  
Thomas T. Chen
Keyword(s):  
Rainbow Trout ◽  
Egg Laying ◽  
Control Fish ◽  
Estradiol Treatment ◽  
Protease Digestion ◽  
Treated Fish ◽  
Estrogen Responsive Gene ◽  
Responsive Gene ◽  
Identification And Characterization

Injection of male rainbow trout with estradiol induced the production of a major serum protein, vitellogenin (VG), of molecular weight (MW) 170 000. This protein is immunoprecipitable by an antiserum raised against lipovitellin from the eggs. Labelling by incubation of liver cubes of estradiol-treated fish with [35S]methionine and analysis of immunospecific products showed incorporation into two polypeptides of MW 170 000 and 160 000. The peptide patterns generated from these two polypeptides by Staphylococcus aureus V8 protease digestion were identical. Cytoplasmic poly(A)+ RNA of estradiol-treated male liver directs the synthesis of a polypeptide of 160 000 daltons in the reticulocyte cell-free protein-synthesizing system or in Xenopus oocytes. This polypeptide is chemically, immunologically, and electrophoretically identical to the authentic VG polypeptide of 160 000 daltons. Changes in mRNA populations in the liver of male fish following estradiol treatment were detected on gels and by hybridization. On methylmercuric hydroxide gels, a polyadenylated RNA species of about 6300 nucleotides was found in estradiol-treated fish which was absent from the control fish. Data from R0t analysis revealed the presence of at least two mRNA sequences of the high frequency class in the RNA of hormone-induced fish and their sizes were determined by Northern blot analysis to be 6300 and 1800 nucleotides, respectively. These observations are consistent with the induction of vitellogenins and at least another estrogen-responsive gene product by estradiol as reported in other egg-laying vertebrates.

Identification and characterization of microRNAs in the liver of rainbow trout in response to heat stress by high-throughput sequencing

Gene ◽  
2018 ◽  
Vol 679 ◽  
pp. 274-281 ◽  
Author(s):  
Jinqiang Huang ◽  
Yongjuan Li ◽  
Fang Ma ◽  
Yujun Kang ◽  
Zhe Liu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Identification And Characterization

scholarly journals Calcineurin, a Calcium/Calmodulin-dependent Protein Phosphatase, Is Involved in Movement, Fertility, Egg Laying, and Growth inCaenorhabditis elegans

2002 ◽  
Vol 13 (9) ◽  
pp. 3281-3293 ◽  
Author(s):  
Jaya Bandyopadhyay ◽  
Jiyeon Lee ◽  
Jungsoo Lee ◽  
Jin Il Lee ◽  
Jae-Ran Yu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Protein Phosphatase ◽  
Body Wall ◽  
Egg Laying ◽  
Α Subunit ◽  
C Elegans ◽  
Calcium Calmodulin Dependent ◽  
Dependent Protein ◽  
Identification And Characterization

Calcineurin is a Ca2+-calmodulin–dependent serine/threonine protein phosphatase that has been implicated in various signaling pathways. Here we report the identification and characterization of calcineurin genes in Caenorhabditis elegans (cna-1 and cnb-1), which share high homology with Drosophila and mammalian calcineurin genes. C. elegans calcineurin binds calcium and functions as a heterodimeric protein phosphatase establishing its biochemical conservation in the nematode. Calcineurin is expressed in hypodermal seam cells, body-wall muscle, vulva muscle, neuronal cells, and in sperm and the spermatheca. cnb-1 mutants showed pleiotropic defects including lethargic movement and delayed egg-laying. Interestingly, these characteristic defects resembled phenotypes observed in gain-of-function mutants ofunc-43/Ca2+-calmodulin–dependent protein kinase II (CaMKII) and goa-1/Go-protein α-subunit. Double mutants of cnb-1 andunc-43(gf) displayed an apparent synergistic severity of movement and egg-laying defects, suggesting that calcineurin may have an antagonistic role in CaMKII-regulated phosphorylation signaling pathways in C. elegans.

scholarly journals Identification and characterization of a novel multi-stress responsive gene in Arabidopsis

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244030
Author(s):  
Faiza Tawab ◽  
Iqbal Munir ◽  
Zeeshan Nasim ◽  
Mohammad Sayyar Khan ◽  
Saleha Tawab ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Survival Rates ◽  
Crop Productivity ◽  
Cell Wall Protein ◽  
Transcriptome Data ◽  
Gus Assay ◽  
Transgenic Lines ◽  
Responsive Gene ◽  
Identification And Characterization

Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.

scholarly journals THE EFFECTS OF BRANCHIAL CHLORIDE CELL PROLIFERATION ON RESPIRATORY FUNCTION IN THE RAINBOW TROUT ONCORHYNCHUS MYKISS

1994 ◽  
Vol 197 (1) ◽  
pp. 47-63
Author(s):  
S Bindon ◽  
K Gilmour ◽  
J Fenwick ◽  
S Perry
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Partial Pressure ◽  
Respiratory Function ◽  
Chloride Cell ◽  
Control Fish ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Arterial Blood ◽  
Environmental Hypoxia ◽  
Treated Fish

The objectives of this study were to induce chloride cell (CC) proliferation on the gill lamellae of rainbow trout Oncorhynchus mykiss and to evaluate the consequences for respiratory function. Chronic elevation of hormone levels was used to induce CC proliferation; fish were injected with a combination of cortisol (8 mg kg-1 intramuscularly every day for 10 days) and ovine growth hormone (2 mg kg-1 intraperitoneally every second day for 10 days). The extent of CC proliferation was quantified using scanning electron microscopy and a two-dimensional analysis. An extracorporeal preparation in combination with environmental hypoxia was used to assess the effects of CC proliferation on respiratory function. Arterial blood was routed from the coeliac artery through an external circuit in which pH (pHa), partial pressure of oxygen (PaO2) and partial pressure of carbon dioxide (PaCO2) were monitored continuously. Environmental hypoxia was imposed by gassing a water equilibration column supplying the experimental chamber with N2. The hormone treatment increased the average CC surface area by 2.7-fold and CC density by 2.2-fold; the combined effect was a fivefold increase in CC fractional area. While the PaO2 values of hormone-treated and control fish were similar at PwO2>12.0 kPa, the arterial O2 tensions of treated fish were significantly lower than those of the control group for PwO2¾12.0 kPa. In comparison with control fish at all environmental O2 tensions, the hormone-treated fish exhibited elevated PaCO2 values and a significant acidosis. The effects of CC proliferation on blood gas variables in hormone-treated fish were accompanied by a significantly elevated ventilation amplitude and a lowered ventilation frequency. The results of this study demonstrated (i) that impairment of respiratory gas transfer coincides with CC proliferation, (ii) that O2 and CO2 transfer are influenced differently and (iii) that partial compensation is achieved through physiological adjustments.

Sign in / Sign up

Export Citation Format

Share Document