DNA methylation in the androgen receptor gene promoter region in rat prostate cancers

The Prostate ◽  
2002 ◽  
Vol 52 (1) ◽  
pp. 82-88 ◽  
Author(s):  
Satoru Takahashi ◽  
Shingo Inaguma ◽  
Michihisa Sakakibara ◽  
Young-Man Cho ◽  
Shugo Suzuki ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Androgen Receptor ◽  
Promoter Region ◽  
Receptor Gene ◽  
Gene Promoter ◽  
Androgen Receptor Gene ◽  
Rat Prostate ◽  
Prostate Cancers ◽  
Gene Promoter Region

Study on DNA Methylation Status of WT1 Gene in Its Promoter Region in Hematologic Neoplasm Cell Lines.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4386-4386
Author(s):  
Ye Zhao ◽  
Zi-xing Chen ◽  
Shao-yan Hu ◽  
Jian-nong Cen
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Cell Lines ◽  
Promoter Region ◽  
U937 Cell ◽  
Methylation Status ◽  
Gene Promoter ◽  
Epigenetic Mechanism ◽  
Wt1 Gene ◽  
Gene Promoter Region

Abstract The methylation at CpG island in the promoter region of a gene is one of the important epigenetic mechanism which regulates the gene activity. To study the DNA methylation pattern of WT1 gene promoter region within hematologic neoplastic cell lines and its correlation with WT1 gene expression by using the PCR-based methods. RT-PCR and Methylation-specific PCR were performed to study the WT1 gene expression in 8226, HL-60, Jurkat, K562, KG-1, NB4, Raji, SHI-1, U266 and U937 cell lines and the DNA methylation status in promoter region of WT1 gene. After treatment of U937 cell line by 5-aza-CdR, a demethylation inducing agent, the changes of WT1 gene expression level and the methylation status in its promter region in U937 cells was determined. Our Results showed that HL-60, K562, KG-1, NB4, SHI-1 cell lines demonstrated higher level of WT1 expression, while extremely low level was found in 8226, Jurkat, Raji, U266 and U937. The DNA hypermethylation in WT1 gene promoter region was identified in 8226, Jurkat, Raji, U266 and U937 cell lines. The WT1 gene expression in U937 was markedly enhanced after treatment with 5-aza-CdR in company with the decrease of methylated level and the increase of unmethylated level in its promoter region. These results indicate that modulation of the DNA methylation in WT1 promoter region is one of the epigenetic mechanisms to regulate its expression.

scholarly journals Epigenetic Regulation of Androgen Receptor Gene Expression in Human Prostate Cancers

2000 ◽  
Vol 80 (12) ◽  
pp. 1789-1796 ◽  
Author(s):  
Tsuyoshi Nakayama ◽  
Masatoshi Watanabe ◽  
Hiroyoshi Suzuki ◽  
Minoru Toyota ◽  
Nobuyuki Sekita ◽  
...  
Keyword(s):  
Gene Expression ◽  
Androgen Receptor ◽  
Epigenetic Regulation ◽  
Receptor Gene ◽  
Androgen Receptor Gene ◽  
Human Prostate ◽  
Prostate Cancers ◽  
Receptor Gene Expression

scholarly journals Negative Regulation of the Androgen Receptor Gene Promoter by NFI and an Adjacently Located Multiprotein-Binding Site

1999 ◽  
Vol 13 (9) ◽  
pp. 1487-1496 ◽  
Author(s):  
Chung S. Song ◽  
Myeong H. Jung ◽  
Prakash C. Supakar ◽  
Bandana Chatterjee ◽  
Arun K. Roy
Keyword(s):  
Androgen Receptor ◽  
Binding Site ◽  
Receptor Gene ◽  
Gene Promoter ◽  
Androgen Receptor Gene ◽  
Negative Regulation

scholarly journals Association of a polymorphism of the 5HT2A receptor gene promoter region with alcohol dependence

1999 ◽  
Vol 4 (1) ◽  
pp. 85-88 ◽  
Author(s):  
T Nakamura ◽  
S Matsushita ◽  
N Nishiguchi ◽  
M Kimura ◽  
A Yoshino ◽  
...  
Keyword(s):  
Alcohol Dependence ◽  
Promoter Region ◽  
Receptor Gene ◽  
Gene Promoter ◽  
5Ht2a Receptor ◽  
Gene Promoter Region
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