An Analytical Method for Assessing Optimal Storage Conditions of Gingival Crevicular Fluid and Disclosing a Peptide Biomarker Signature of Gingivitis by MALDI-TOF MS

2018 ◽  
Vol 12 (5) ◽  
pp. 1800005 ◽  
Author(s):  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Maria Stella Murfuni ◽  
Chiara Villella ◽  
Corrado Pelaia ◽  
...  
Keyword(s):  
Analytical Method ◽  
Gingival Crevicular Fluid ◽  
Storage Conditions ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Crevicular Fluid ◽  
Biomarker Signature ◽  
Tof Ms

Influence of storage conditions on MALDI-TOF MS profiling of gingival crevicular fluid: Implications on the role of S100A8 and S100A9 for clinical and proteomic based diagnostic investigations

PROTEOMICS ◽  
2016 ◽  
Vol 16 (6) ◽  
pp. 1033-1045 ◽  
Author(s):  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Nicola Lombardo ◽  
Tiziana Montalcini ◽  
Sergio Paduano ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Storage Conditions ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Crevicular Fluid ◽  
Tof Ms ◽  
Diagnostic Investigations

scholarly journals Optimization and Standardization of Human Saliva Collection for MALDI-TOF MS

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1304
Author(s):  
Monique Melo Costa ◽  
Nicolas Benoit ◽  
Florian Saby ◽  
Bruno Pradines ◽  
Samuel Granjeaud ◽  
...  
Keyword(s):  
Low Cost ◽  
Storage Conditions ◽  
Human Saliva ◽  
Learning Approaches ◽  
Sample Collection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Key Factor ◽  
Diagnosis Method ◽  
Tof Ms

SARS-CoV-2 outbreak led to unprecedented innovative scientific research to preclude the virus dissemination and limit its impact on life expectancy. Waiting for the collective immunity by vaccination, mass-testing, and isolation of positive cases remain essential. The development of a diagnosis method requiring a simple and non-invasive sampling with a quick and low-cost approach is on demand. We hypothesized that the combination of saliva specimens with MALDI-TOF MS profiling analyses could be the winning duo. Before characterizing MS saliva signatures associated with SARS-CoV-2 infection, optimization and standardization of sample collection, preparation and storage up to MS analyses appeared compulsory. In this view, successive experiments were performed on saliva from healthy healthcare workers. Specimen sampling with a roll cotton of Salivette® devices appeared the most appropriate collection mode. Saliva protein precipitation with organic buffers did not improved MS spectra profiles compared to a direct loading of samples mixed with acetonitrile/formic acid buffer onto MS plate. The assessment of sample storage conditions and duration revealed that saliva should be stored on ice until MS analysis, which should occur on the day of sampling. Kinetic collection of saliva highlighted reproducibility of saliva MS profiles over four successive days and also at two-week intervals. The intra-individual stability of saliva MS profiles should be a key factor in the future investigation for biomarkers associated with SARS-CoV-2 infection. However, the singularity of MS profiles between individuals will require the development of sophisticated bio-statistical analyses such as machine learning approaches. MALDI-TOF MS profiling of saliva could be a promising PCR-free tool for SARS-CoV-2 screening.

Comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by MALDI-TOF MS

2017 ◽  
Vol 31 (4) ◽  
pp. 438-448 ◽  
Author(s):  
A. NEBBAK ◽  
B. EL HAMZAOUI ◽  
J.-M. BERENGER ◽  
I. BITAM ◽  
D. RAOULT ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Storage Conditions ◽  
Flea Species ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Homogenization Methods ◽  
Tof Ms

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Quantitative metabolomics of Iranian saffron based on their HPLC-DAD and MALDI-TOF-MS

2019 ◽  
Author(s):  
M Hooshyari ◽  
H Rezadoost ◽  
P Ghezellou ◽  
A Ghassempour
Keyword(s):  
Maldi Tof Ms ◽  
Quantitative Metabolomics ◽  
Maldi Tof ◽  
Tof Ms
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