Inside front cover: Influence of Storage Conditions on MALDI-TOF MS Profiling of Gingival Crevicular Fluid: Implications on the Role of S100A8 and S100A9 for Clinical and Proteomic based Diagnostic Investigations

PROTEOMICS ◽  
2016 ◽  
Vol 16 (6) ◽  
pp. NA-NA
Author(s):  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Nicola Lombardo ◽  
Tiziana Montalcini ◽  
Sergio Paduano ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Storage Conditions ◽  
Front Cover ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Crevicular Fluid ◽  
Tof Ms ◽  
Diagnostic Investigations

Influence of storage conditions on MALDI-TOF MS profiling of gingival crevicular fluid: Implications on the role of S100A8 and S100A9 for clinical and proteomic based diagnostic investigations

PROTEOMICS ◽  
2016 ◽  
Vol 16 (6) ◽  
pp. 1033-1045 ◽  
Author(s):  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Nicola Lombardo ◽  
Tiziana Montalcini ◽  
Sergio Paduano ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Storage Conditions ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Crevicular Fluid ◽  
Tof Ms ◽  
Diagnostic Investigations

An Analytical Method for Assessing Optimal Storage Conditions of Gingival Crevicular Fluid and Disclosing a Peptide Biomarker Signature of Gingivitis by MALDI-TOF MS

2018 ◽  
Vol 12 (5) ◽  
pp. 1800005 ◽  
Author(s):  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Maria Stella Murfuni ◽  
Chiara Villella ◽  
Corrado Pelaia ◽  
...  
Keyword(s):  
Analytical Method ◽  
Gingival Crevicular Fluid ◽  
Storage Conditions ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Crevicular Fluid ◽  
Biomarker Signature ◽  
Tof Ms

Update on the Evolving Role of MALDI-TOF MS for Laboratory Diagnosis of Fungal Infections

2012 ◽  
Vol 6 (3) ◽  
pp. 206-214 ◽  
Author(s):  
Edith Vermeulen ◽  
Jan Verhaegen ◽  
Christophe Indevuyst ◽  
Katrien Lagrou
Keyword(s):  
Fungal Infections ◽  
Laboratory Diagnosis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Implementation of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase-Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis

2015 ◽  
Vol 53 (7) ◽  
pp. 2030-2036 ◽  
Author(s):  
Xavier Argemi ◽  
Philippe Riegel ◽  
Thierry Lavigne ◽  
Nicolas Lefebvre ◽  
Nicolas Grandpré ◽  
...  
Keyword(s):  
Laser Desorption Ionization ◽  
Coagulase Negative Staphylococci ◽  
Ionization Time ◽  
Content Type ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Phenotypic Methods ◽  
Tof Ms

The use of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for staphylococcal identification is now considered routine in laboratories compared with the conventional phenotypical methods previously used. We verified its microbiological relevance for identifying the main species of coagulase-negative staphylococci (CoNS) by randomly selecting 50 isolates. From 1 January 2007 to 31 August 2008, 12,479 staphylococci were isolated with phenotypic methods, of which 4,594 were identified asStaphylococcus aureusand 7,885 were coagulase negative staphylococci. Using MALDI-TOF MS from 1 January 2011 to 31 August 2012, 14,913 staphylococci were identified, with 5,066 asS. aureusand 9,847 as CoNS. MALDI-TOF MS allowed the identification of approximately 85% of the CoNS strains, whereas only 14% of the CoNS strains were identified to the species level with phenotypic methods because they were often considered contaminants. Furthermore, the use of MALDI-TOF MS revealed the occurrence of recently characterizedStaphylococcusspecies, such asS. pettenkoferi,S. condimenti, andS. piscifermentans. Microbiological relevance analysis further revealed that some species displayed a high rate of microbiological significance, i.e., 40% of theS. lugdunensisstrains included in the analysis were associated with infection risk. This retrospective microbiological study confirms the role of MALDI-TOF MS in clinical settings for the identification of staphylococci with clinical consequences. The species distribution reveals the occurrence of the recently identified speciesS. pettenkoferiand putative virulent species, includingS. lugdunensis.

scholarly journals Optimization and Standardization of Human Saliva Collection for MALDI-TOF MS

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1304
Author(s):  
Monique Melo Costa ◽  
Nicolas Benoit ◽  
Florian Saby ◽  
Bruno Pradines ◽  
Samuel Granjeaud ◽  
...  
Keyword(s):  
Low Cost ◽  
Storage Conditions ◽  
Human Saliva ◽  
Learning Approaches ◽  
Sample Collection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Key Factor ◽  
Diagnosis Method ◽  
Tof Ms

SARS-CoV-2 outbreak led to unprecedented innovative scientific research to preclude the virus dissemination and limit its impact on life expectancy. Waiting for the collective immunity by vaccination, mass-testing, and isolation of positive cases remain essential. The development of a diagnosis method requiring a simple and non-invasive sampling with a quick and low-cost approach is on demand. We hypothesized that the combination of saliva specimens with MALDI-TOF MS profiling analyses could be the winning duo. Before characterizing MS saliva signatures associated with SARS-CoV-2 infection, optimization and standardization of sample collection, preparation and storage up to MS analyses appeared compulsory. In this view, successive experiments were performed on saliva from healthy healthcare workers. Specimen sampling with a roll cotton of Salivette® devices appeared the most appropriate collection mode. Saliva protein precipitation with organic buffers did not improved MS spectra profiles compared to a direct loading of samples mixed with acetonitrile/formic acid buffer onto MS plate. The assessment of sample storage conditions and duration revealed that saliva should be stored on ice until MS analysis, which should occur on the day of sampling. Kinetic collection of saliva highlighted reproducibility of saliva MS profiles over four successive days and also at two-week intervals. The intra-individual stability of saliva MS profiles should be a key factor in the future investigation for biomarkers associated with SARS-CoV-2 infection. However, the singularity of MS profiles between individuals will require the development of sophisticated bio-statistical analyses such as machine learning approaches. MALDI-TOF MS profiling of saliva could be a promising PCR-free tool for SARS-CoV-2 screening.

Comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by MALDI-TOF MS

2017 ◽  
Vol 31 (4) ◽  
pp. 438-448 ◽  
Author(s):  
A. NEBBAK ◽  
B. EL HAMZAOUI ◽  
J.-M. BERENGER ◽  
I. BITAM ◽  
D. RAOULT ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Storage Conditions ◽  
Flea Species ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Homogenization Methods ◽  
Tof Ms

MALDI-TOF/MS biomarkers discovery for pancreatic cancer diagnosis: emerging role of Apolipoprotein A1 and complement C3

Pancreatology ◽  
2012 ◽  
Vol 12 (6) ◽  
pp. 505
Author(s):  
A. Padoan ◽  
R. Seraglia ◽  
P. Fogar ◽  
C. Sperti ◽  
S. Moz ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Diagnosis ◽  
Apolipoprotein A1 ◽  
Complement C3 ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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