High-abundance protein depletion: Comparison of methods for human plasma biomarker discovery

2010 ◽  
Vol 4 (8-9) ◽  
pp. 774-774
Author(s):  
Veronika Polaskova ◽  
Amit Kapur ◽  
Alamgir Khan ◽  
Mark P. Molloy ◽  
Mark S. Baker
Keyword(s):  
Human Plasma ◽  
Biomarker Discovery ◽  
High Abundance ◽  
Comparison Of Methods ◽  
Plasma Biomarker ◽  
Protein Depletion ◽  
High Abundance Protein

High-abundance protein depletion: Comparison of methods for human plasma biomarker discovery

2010 ◽  
Vol 31 (3) ◽  
pp. 471-482 ◽  
Author(s):  
Veronika Polaskova ◽  
Amit Kapur ◽  
Alamgir Khan ◽  
Mark P. Molloy ◽  
Mark S. Baker
Keyword(s):  
Human Plasma ◽  
Biomarker Discovery ◽  
High Abundance ◽  
Comparison Of Methods ◽  
Plasma Biomarker ◽  
Protein Depletion ◽  
High Abundance Protein

Quantitative assessment of human serum high-abundance protein depletion

2008 ◽  
Vol 29 (21) ◽  
pp. 4316-4323 ◽  
Author(s):  
Rene Stempfer ◽  
Markus Kubicek ◽  
Irene M. Lang ◽  
Noehammer Christa ◽  
Christopher Gerner
Keyword(s):  
Human Serum ◽  
Quantitative Assessment ◽  
High Abundance ◽  
Protein Depletion ◽  
High Abundance Protein

Comparison of diverse affinity based high-abundance protein depletion strategies for improved bio-marker discovery in oral fluids

2012 ◽  
Vol 75 (13) ◽  
pp. 4165-4175 ◽  
Author(s):  
Guy Krief ◽  
Omer Deutsch ◽  
Batia Zaks ◽  
David T. Wong ◽  
Doron J. Aframian ◽  
...  
Keyword(s):  
High Abundance ◽  
Protein Depletion ◽  
Oral Fluids ◽  
High Abundance Protein ◽  
Marker Discovery

scholarly journals Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

2011 ◽  
Vol 9 (1) ◽  
pp. 24 ◽  
Author(s):  
Bin Liu ◽  
Fang-hua Qiu ◽  
Courtney Voss ◽  
Yun Xu ◽  
Ming-zhe Zhao ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
High Abundance ◽  
Protein Depletion ◽  
Cord Serum ◽  
Serum Proteomics ◽  
High Abundance Protein

scholarly journals Evaluation of two high-abundance protein depletion kits and optimization of downstream isoelectric focusing

2015 ◽  
Vol 12 (5) ◽  
pp. 7749-7755 ◽  
Author(s):  
FANGHUA QIU ◽  
TIEYING HOU ◽  
DEHONG HUANG ◽  
ZHIFENG XUE ◽  
DONGYAN LIANG ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
High Abundance ◽  
Protein Depletion ◽  
High Abundance Protein

scholarly journals The protocol to remove high abundant proteins from HIV-1 infected human plasma

2019 ◽  
Author(s):  
Sushanta Kumar Barik ◽  
Keshar Kunja Mohanty ◽  
Deepa Bisht ◽  
Partha Sarathi Mohanty ◽  
Shripad Patil ◽  
...  
Keyword(s):  
Human Plasma ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
High Abundance ◽  
Plasma Samples ◽  
Human Plasma Samples ◽  
High Abundance Protein ◽  
Hiv 1 ◽  
Poly Acrylamide

Abstract Removal of high abundance protein from human plasma is an advantage during the analysis of protein in SDS-Poly acrylamide gel electrophoresis. We have adopted a protocol for removal of high abundance protein from human plasma by following several previous protocols. The 50% TCA-Acetone is most suitable method for removal of high abundance proteins like albumin and globulin from HIV-1 infected human plasma samples. We have developed this protocol in our laboratory for carrying out the protein work from HIV-1 infected human plasma samples. This protocol is suitable to run the purified human plasma protein for sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Novel Strategy of High-Abundance Protein Depletion Using Multidimensional Liquid Chromatography

2006 ◽  
Vol 5 (10) ◽  
pp. 2853-2860 ◽  
Author(s):  
Mingxia Gao ◽  
Jie Zhang ◽  
Chunhui Deng ◽  
Pengyuan Yang ◽  
Xiangmin Zhang
Keyword(s):  
Liquid Chromatography ◽  
High Abundance ◽  
Protein Depletion ◽  
High Abundance Protein ◽  
Novel Strategy
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