Exhaled breath condensate in pediatric asthma: Promising new advance or pouring cold water on a lot of hot air? A systematic review

2013 ◽  
Vol 48 (5) ◽  
pp. 419-442 ◽  
Author(s):  
P.S. Thomas ◽  
A.J. Lowe ◽  
P. Samarasinghe ◽  
C.J. Lodge ◽  
Y. Huang ◽  
...  
Keyword(s):  
Systematic Review ◽  
Cold Water ◽  
Exhaled Breath Condensate ◽  
Pediatric Asthma ◽  
Exhaled Breath ◽  
Hot Air ◽  
Breath Condensate

scholarly journals Biomarkers in adult asthma: a systematic review of 8-isoprostane in exhaled breath condensate

2017 ◽  
Vol 11 (1) ◽  
pp. 016011 ◽  
Author(s):  
Adam M Peel ◽  
Christina-Jane Crossman-Barnes ◽  
Jonathan Tang ◽  
Stephen J Fowler ◽  
Gwyneth A Davies ◽  
...  
Keyword(s):  
Systematic Review ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Adult Asthma ◽  
Breath Condensate

Exhaled breath condensate cytokines and pH in pediatric asthma and atopic dermatitis

2008 ◽  
Vol 29 (5) ◽  
pp. 461-467 ◽  
Author(s):  
Luigia Brunetti ◽  
Ruggiero Francavilla ◽  
Riccardina Tesse ◽  
Patrizia Fiermonte ◽  
Francesca Paola Fiore ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Exhaled Breath Condensate ◽  
Pediatric Asthma ◽  
Exhaled Breath ◽  
Breath Condensate

scholarly journals S1 Biomarkers in adult asthma: a systematic review of 8-isoprostane in exhaled breath condensate

Thorax ◽  
2016 ◽  
Vol 71 (Suppl 3) ◽  
pp. A4.1-A4
Author(s):  
AM Peel ◽  
CJ Crossman-Barnes ◽  
J Tang ◽  
SJ Fowler ◽  
GA Davies ◽  
...  
Keyword(s):  
Systematic Review ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Adult Asthma ◽  
Breath Condensate

scholarly journals Reference Ranges of 8-Isoprostane Concentrations in Exhaled Breath Condensate (EBC): A Systematic Review and Meta-Analysis

2020 ◽  
Vol 21 (11) ◽  
pp. 3822 ◽  
Author(s):  
Yara Shoman ◽  
Pascal Wild ◽  
Maud Hemmendinger ◽  
Melanie Graille ◽  
Jean-Jacques Sauvain ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Analytical Methods ◽  
Reference Range ◽  
Exhaled Breath Condensate ◽  
Meta Analysis ◽  
Individual Characteristics ◽  
Exhaled Breath ◽  
Reference Ranges ◽  
Breath Condensate

Isoprostanes are physiopathologic mediators of oxidative stress, resulting in lipid peroxidation. 8-isoprostane seems particularly useful for measuring oxidative stress damage. However, no reference range values are available for 8-isoprosante in exhaled breath condensate (EBC) of healthy adults, enabling its meaningful interpretation as a biomarker. We conducted this systematic review and meta-analysis according to the protocol following PROSPERO (CRD42020146623). After searching and analyzing the literature, we included 86 studies. After their qualitative synthesis and risk of bias assessment, 52 studies were included in meta-analysis. The latter focused on studies using immunological analytical methods and investigated how the concentrations of 8-isoprostane differ based on gender. We found that gender had no significant effect in 8-isoprostane concentration. Among other studied factors, such as individual characteristics and factors related to EBC collection, only the device used for EBC collection significantly affected measured 8-isoprostane concentrations. However, adjustment for the factors related to EBC collection, yielded uncertainty whether this effect is due to the device itself or to the other factors. Given this uncertainty, we estimated the reference range values of 8-isoprostane stratified by gender and EBC collection device. A better standardization of EBC collection seems necessary; as well more studies using chemical analytical methods to extend this investigation.

scholarly journals Relationships between adult asthma and oxidative stress markers and pH in exhaled breath condensate: a systematic review

Allergy ◽  
2016 ◽  
Vol 71 (6) ◽  
pp. 741-757 ◽  
Author(s):  
F. M. Aldakheel ◽  
P. S. Thomas ◽  
J. E. Bourke ◽  
M. C. Matheson ◽  
S. C. Dharmage ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Oxidative Stress Markers ◽  
Adult Asthma ◽  
Stress Markers ◽  
Breath Condensate ◽  
And Oxidative Stress

Preliminary Analysis of the Proteome of Exhaled Breath Condensate in Bottlenose Dolphins (Tursiops truncatus)

2018 ◽  
Vol 44 (3) ◽  
pp. 256-266 ◽  
Author(s):  
Don R. Bergfelt ◽  
John Lippolis ◽  
Michel Vandenplas ◽  
Sydney Davis ◽  
Blake A. Miller ◽  
...  
Keyword(s):  
Preliminary Analysis ◽  
Tursiops Truncatus ◽  
Exhaled Breath Condensate ◽  
Bottlenose Dolphins ◽  
Exhaled Breath ◽  
Breath Condensate

Simultaneous and Sensitive Determination of Amphetamine, Codeine and Morphine in Exhaled Breath Condensate, Using Capillary Electrophoresis Coupled with On-line and Off-line Enhancing Methods

2020 ◽  
Vol 16 (7) ◽  
pp. 872-879
Author(s):  
Samin Hamidi
Keyword(s):  
Drugs Of Abuse ◽  
Exhaled Breath Condensate ◽  
Direct Injection ◽  
Forensic Toxicology ◽  
Analytical Signal ◽  
Exhaled Breath ◽  
Concentration Method ◽  
Validation Parameters ◽  
Breath Condensate

Background: Abuse of drugs is associated with several medical, forensic, toxicology and social challenges. “Drugs of abuse” testing is therefore an important issue. Objective: We propose a simple CE-based method for the quantification of amphetamine, codeine and morphine after direct injection of Exhaled Breath Condensate (EBC) by the aid of simple stacking mode and an off-line pre-concentration method. Methods: Using graphene oxide adsorbents, amphetamine, codeine and morphine were extracted from EBC in order to eliminate the proteins and other interferences. In addition to off-line method, an online stacking mode was applied to improve the analytical signal obtained from the instrument. Results: The validation parameters were experimented on the developed method based on the FDA guideline over concentration ranges of 12.5-100, 30-500 and 10-1250 ng/mL associated with amphetamine, codeine and morphine, respectively. Small volumes (around 100 μL) of EBC were collected using a lab-made setup and successfully analyzed using the proposed method where precisions and accuracies (within day and between days) were in accordance with the guideline (recommended less than 15 % for biological samples). The recovery tests were used to evaluate the matrix effect and data (94 to 105 %) showed that the proposed method can be applied in different EBC matrix samplings of subjects. Conclusion: The proposed method is superior for simultaneous determination of amphetamine, codeine and morphine over chromatographic analyses because it is fast and consumes fewer chemicals, with no derivatization step.

Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

2020 ◽  
Vol 16 (8) ◽  
pp. 1032-1040
Author(s):  
Laleh Samini ◽  
Maryam Khoubnasabjafari ◽  
Mohamad M. Alimorad ◽  
Vahid Jouyban-Gharamaleki ◽  
Hak-Kim Chan ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Exhaled Breath Condensate ◽  
Low Cost ◽  
Exhaled Breath ◽  
Chromatography Method ◽  
Extraction Solvent ◽  
Drug Concentrations ◽  
Dispersive Liquid Liquid Microextraction ◽  
Breath Condensate

Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations. Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS. Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples. Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.

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