Assembly of Gold Nanoparticles on Gold Nanorods Using Functionalized Poly(N-isopropylacrylamide) as Polymeric “Glue”

2016 ◽  
Vol 33 (9) ◽  
pp. 698-702 ◽  
Author(s):  
Ziyin Fan ◽  
Moritz Tebbe ◽  
Andreas Fery ◽  
Seema Agarwal ◽  
Andreas Greiner
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanorods

scholarly journals Uniformly Immobilizing Gold Nanorods on a Glass Substrate

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Hadas Weinrib ◽  
Amihai Meiri ◽  
Hamootal Duadi ◽  
Dror Fixler
Keyword(s):  
Image Processing ◽  
Gold Nanoparticles ◽  
Gold Nanorods ◽  
Glass Substrate ◽  
Average Density ◽  
Glass Slide ◽  
Vertical Position ◽  
Analysis Software ◽  
Image Processing And Analysis

The goal of this paper is to immobilize gold nanoparticles uniformly on a glass substrate. In order to attach gold-nanorods (GNRs) to an area of a few squared microns surface of glass substrate without preliminary coating of the GNR, 3-(Mercaptopropyl)trimethoxysilane molecules were used as linker while using different methods. These methods included placing the glass slide and the GNR (1) inside a tube without any motion; (2) inside a shaker; (3) in a fan setup. The fan setup included a tube containing the GNR solution and the glass slide at a vertical position, when the fan blows above the tube, producing turbulations in the liquid. Each method was evaluated according to the density and the homogeneousness of the GNR monolayer on the surface. The uniformity of the monolayer was demonstrated using AFM images of different areas on the slides, and the effectiveness of the protocol was demonstrated by calculating the average density of the GNR on the surface using image processing and analysis software. It was found that while both the shaker and the fan setups improved the monolayer density, the fan setup improved the density by a factor of more than two than the density found using the shaker.

scholarly journals Gold Nanorod Bioconjugates for Active Tumor Targeting and Photothermal Therapy

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Hadiyah N. Green ◽  
Dmitry V. Martyshkin ◽  
Cynthia M. Rodenburg ◽  
Eben L. Rosenthal ◽  
Sergey B. Mirov
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Tumor Targeting ◽  
Near Infrared ◽  
Malignant Tumors ◽  
Photothermal Effect ◽  
Specific Antibodies ◽  
Before And After ◽  
Egfr Antibody

The mastery of active tumor targeting is a great challenge in near infrared photothermal therapy (NIRPTT). To improve efficiency for targeted treatment of malignant tumors, we modify the technique of conjugating gold nanoparticles to tumor-specific antibodies. Polyethylene glycol-coated (PEGylated) gold nanorods (GNRs) were fabricated and conjugated to an anti-EGFR antibody. We characterized the conjugation efficiency of the GNRs by comparing the efficiency of antibody binding and the photothermal effect of the GNRs before and after conjugation. We demonstrate that the binding efficiency of the antibodies conjugated to the PEGylated GNRs is comparable to the binding efficiency of the unmodified antibodies and 33.9% greater than PEGylated antibody-GNR conjugates as reported by Liao and Hafner (2005). In addition, cell death by NIRPTT was sufficient to kill nearly 90% of tumor cells, which is comparable to NIRPTT with GNRs alone confirming that NIRPTT using GNRs is not compromised by conjugation of GNRs to antibodies.

Delivery and cytotoxicity of doxorubicin and temozolomide to primary glioblastoma cells using gold nanospheres and gold nanorods

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Jyoti Verma ◽  
Henk A. Van Veen ◽  
Sumit Lal ◽  
Cornelis J.F. Van Noorden
Keyword(s):  
Gold Nanoparticles ◽  
Cell Cultures ◽  
Gold Nanorods ◽  
Silica Coating ◽  
Glioblastoma Cell ◽  
Glioblastoma Cells ◽  
Gold Nanospheres ◽  
Primary Glioblastoma ◽  
Direct Delivery

AbstractNanoparticles with coating entrapping a chemotherapeutic drug for delivery have not been tested for their cytotoxic effects in in-vitro glioblastoma cell cultures to increase treatment efficacy. Therefore, we synthesized silica-coated gold nanorods and gold nanospheres that were loaded with doxorubicin or temozolomide. The morphology of the nanoparticles was characterized using transmission electron microscopy (TEM), the molecular structure was characterized using infrared spectroscopy and in vitro efficacy was determined using glioblastoma cell cultures. TEM analysis showed that gold nanorods had a length of 49–65 nm and a diameter of 8.5–14 nm whereas gold nanospheres had a diameter of 9.5–37 nm. Infrared spectroscopy of doxorubicin and temozolomide and the silica coating revealed molecular fingerprints such as bending, stretching and vibrations of chemical bonds that confirmed the presence of silica coating and drug loading of the gold nanoparticles. In the biological assessment of the effects of drug-loaded gold nanoparticles on primary glioblastoma cell cultures, cytotoxicity, viability and the ratio of cyototoxicity and viability were used as parameters to analyze the effects on the cells of drug delivery via gold nanoparticles on the cells. Our data suggest that doxorubicin in the concentration range of 0.12–3.16 μM when delivered using both gold nanorods and nanospheres induced a 3.8–5.5-fold increased cytotoxicity in comparison to direct delivery. Temozolomide in the concentration range of 4.6–115 μM when delivered by either type of gold nanoparticles induced a 2–4-fold increased cytotoxicity in comparison to direct delivery. Nanospheres were more effective in delivery and cytotoxicity of doxorubicin and temozolomide to glioblastoma cells than gold nanorods. Our data suggest that gold nanoparticles and in particular gold nanospheres are more effective in delivery of doxorubicin and temozolomide to primary glioblastoma cells in culture than direct delivery.

Spherical gold nanoparticles and gold nanorods for the determination of gentamicin

2017 ◽  
Vol 172 ◽  
pp. 126-134 ◽  
Author(s):  
Jarol R. Miranda-Andrades ◽  
Aurora Pérez-Gramatges ◽  
Omar Pandoli ◽  
Eric C. Romani ◽  
Ricardo Q. Aucélio ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanorods ◽  
Spherical Gold Nanoparticles

scholarly journals Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV CP

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2628
Author(s):  
Ana L. Durán-Meza ◽  
Martha I. Escamilla-Ruiz ◽  
Xochitl F. Segovia-González ◽  
Maria V. Villagrana-Escareño ◽  
J. Roger Vega-Acosta ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Charge ◽  
Gold Nanorods ◽  
Self Assembly ◽  
Ostwald Ripening ◽  
Gold Nanoshells ◽  
Medical Applications ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Different types of gold nanoparticles have been synthesized that show great potential in medical applications such as medical imaging, bio-analytical sensing and photothermal cancer therapy. However, their stability, polydispersity and biocompatibility are major issues of concern. For example, the synthesis of gold nanorods, obtained through the elongated micelle process, produce them with a high positive surface charge that is cytotoxic, while gold nanoshells are unstable and break down in a few weeks due to the Ostwald ripening process. In this work, we report the self-assembly of the capsid protein (CP) of cowpea chlorotic mottle virus (CCMV) around spherical gold nanoparticles, gold nanorods and gold nanoshells to form virus-like particles (VLPs). All gold nanoparticles were synthesized or treated to give them a negative surface charge, so they can interact with the positive N-terminus of the CP leading to the formation of the VLPs. To induce the protein self-assembly around the negative gold nanoparticles, we use different pH and ionic strength conditions determined from a CP phase diagram. The encapsidation with the viral CP will provide the nanoparticles better biocompatibility, stability, monodispersity and a new biological substrate on which can be introduced ligands toward specific cells, broadening the possibilities for medical applications.

Gold nanoparticles impair autophagy flux through shape-dependent endocytosis and lysosomal dysfunction

2018 ◽  
Vol 6 (48) ◽  
pp. 8127-8136 ◽  
Author(s):  
Hualu Zhou ◽  
Xuanqing Gong ◽  
Hongyu Lin ◽  
Hongming Chen ◽  
Dengtong Huang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Gold Nanospheres ◽  
Autophagy Flux ◽  
Lysosomal Dysfunction

Gold nanospheres stimulate more autophagosome accumulation than gold nanorods due to their higher efficiency of cellular uptake.

scholarly journals Photostability of gold nanoparticles with different shapes: the role of Ag clusters

Nanoscale ◽  
2015 ◽  
Vol 7 (26) ◽  
pp. 11273-11279 ◽  
Author(s):  
Yasser A. Attia ◽  
David Buceta ◽  
Félix G. Requejo ◽  
Lisandro J. Giovanetti ◽  
M. Arturo López-Quintela
Keyword(s):  
Gold Nanoparticles ◽  
Low Power ◽  
Uv Irradiation ◽  
Gold Nanorods ◽  
Ag Clusters ◽  
Different Shapes

Involvement of semiconductor-like Ag clusters in the photodissolution of gold nanorods and nanoprisms by low-power UV irradiation.

Effects of bipyramidal gold nanoparticles and gold nanorods on the detection of immunoglobulins

The Analyst ◽  
2016 ◽  
Vol 141 (21) ◽  
pp. 6080-6086 ◽  
Author(s):  
Hua Zhang ◽  
Zhe She ◽  
Han Su ◽  
Kagan Kerman ◽  
Heinz-Bernhard Kraatz
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanorods ◽  
Model System ◽  
System P ◽  
Different Shapes

The influence of different shapes of gold nanoparticles and techniques (SPR and SWV) on the sensitivity of biosensors was investigated, using IgG detection as a model system.

Effects of Size, Shape, Surface Charge and Functionalization on Cytotoxicity of Gold Nanoparticles

Nano LIFE ◽  
2015 ◽  
Vol 05 (01) ◽  
pp. 1540003 ◽  
Author(s):  
Zhuheng Li ◽  
Zhen Lei ◽  
Junping Zhang ◽  
Dianjun Liu ◽  
Zhenxin Wang
Keyword(s):  
Gold Nanoparticles ◽  
Data Analysis ◽  
Physicochemical Properties ◽  
Surface Charge ◽  
Gold Nanorods ◽  
Gold Nanoclusters ◽  
Gold Nanoshells ◽  
Cell Models ◽  
Future Prospects ◽  
Au Nps

Gold nanoparticles ( Au NPs) are emerging as promising nanomaterials from which we construct diagnostic and therapeutic nanosystems. For understanding the fundamental behaviors of Au NPs with biological systems, interactions of Au NPs and cells should be considered first. In this review, we present a detailed analysis of data on the cytotoxicity of most popular Au NPs including gold nanoclusters ( Au NCs), spherical Au NPs, gold nanoshells ( Au NSs) and gold nanorods ( Au NRs). Relationships correlating the cell models, physicochemical properties (size, shape, chemical functionality and surface charge) of Au NPs and cytotoxicity are discussed on the basis of data analysis. Some general conclusions, current challenges and future prospects/solutions on the issue have been provided.

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