Cytotoxicity and Cellular Uptake of Amorphous Silica Nanoparticles in Human Cancer Cells

2015 ◽  
Vol 32 (7) ◽  
pp. 779-787 ◽  
Author(s):  
Yuequn Wu ◽  
Wei Tang ◽  
Peng Wang ◽  
Changsheng Liu ◽  
Yuan Yuan ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Amorphous Silica ◽  
Human Cancer ◽  
Human Cancer Cells

scholarly journals Ruthenium-based PACT agents based on bisquinoline chelates: synthesis, photochemistry, and cytotoxicity

2021 ◽  
Author(s):  
Anja Busemann ◽  
Ingrid Flaspohler ◽  
Xue-Quan Zhou ◽  
Claudia Schmidt ◽  
Sina K. Goetzfried ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Singlet Oxygen ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Ruthenium Complex ◽  
Human Cancer ◽  
Light Irradiation ◽  
Quantum Yields ◽  
Light Activation ◽  
Human Cancer Cells

AbstractThe known ruthenium complex [Ru(tpy)(bpy)(Hmte)](PF6)2 ([1](PF6)2, where tpy = 2,2’:6’,2″-terpyridine, bpy = 2,2’-bipyridine, Hmte = 2-(methylthio)ethanol) is photosubstitutionally active but non-toxic to cancer cells even upon light irradiation. In this work, the two analogs complexes [Ru(tpy)(NN)(Hmte)](PF6)2, where NN = 3,3'-biisoquinoline (i-biq, [2](PF6)2) and di(isoquinolin-3-yl)amine (i-Hdiqa, [3](PF6)2), were synthesized and their photochemistry and phototoxicity evaluated to assess their suitability as photoactivated chemotherapy (PACT) agents. The increase of the aromatic surface of [2](PF6)2 and [3](PF6)2, compared to [1](PF6)2, leads to higher lipophilicity and higher cellular uptake for the former complexes. Such improved uptake is directly correlated to the cytotoxicity of these compounds in the dark: while [2](PF6)2 and [3](PF6)2 showed low EC50 values in human cancer cells, [1](PF6)2 is not cytotoxic due to poor cellular uptake. While stable in the dark, all complexes substituted the protecting thioether ligand upon light irradiation (520 nm), with the highest photosubstitution quantum yield found for [3](PF6)2 (Φ[3] = 0.070). Compounds [2](PF6)2 and [3](PF6)2 were found both more cytotoxic after light activation than in the dark, with a photo index of 4. Considering the very low singlet oxygen quantum yields of these compounds, and the lack of cytotoxicity of the photoreleased Hmte thioether ligand, it can be concluded that the toxicity observed after light activation is due to the photoreleased aqua complexes [Ru(tpy)(NN)(OH2)]2+, and thus that [2](PF6)2 and [3](PF6)2 are promising PACT candidates. Graphic abstract

Uptake of Functionalized Mesoporous Silica Nanoparticles by Human Cancer Cells

2010 ◽  
Vol 10 (4) ◽  
pp. 2314-2324 ◽  
Author(s):  
Matthieu Fisichella ◽  
Hinda Dabboue ◽  
Sanjib Bhattacharyya ◽  
Gérald Lelong ◽  
Marie-Louise Saboungi ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Human Cancer ◽  
Mesoporous Silica Nanoparticles ◽  
Human Cancer Cells ◽  
Functionalized Mesoporous Silica

scholarly journals Carvacrol effect on topotecan cytotoxicity in various human cancer cells in vitro

Pharmacia ◽  
2021 ◽  
Vol 68 (2) ◽  
pp. 353-363
Author(s):  
Hadeel M. Bayoumi ◽  
Mayson H. Alkhatib ◽  
Madeha N. Al-Seeni
Keyword(s):  
Combination Therapy ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Human Cancer ◽  
A549 Cells ◽  
Single Treatment ◽  
Human Cancer Cells ◽  
Coomassie Blue ◽  
Mcf 7

Purpose: To investigate the modulatory effect of the natural phytochemical, carvacrol, on Topotecan (TOPO) cytotoxicity and cellular uptake in different cancer cell lines. Methods: The cytotoxicity of the carvacrol/TOPO combination therapy was determined in vitro using crystal violet assay. Coomassie blue and DAPI fluorescent stains were used for cellular morphology and molecular cell death assessments, respectively. Additionally, TOPO cellular uptake after carvacrol/TOPO combination therapy was determined. Results: Treatment of HeLa and HCT116 with carvacrol/TOPO resulted in 7.70- and 5.71-fold reduction in TOPO half maximal inhibitory concentration (IC50), respectively, relative to TOPO single treatment. On the other hand, treatment of MCF-7, HepG2, SKOV3, and A549 cancer cells with carvacrol/TOPO resulted in increasing the IC50 of TOPO by 1.49-, 1.33-, 1.50- and 1.26-fold, respectively, relative to TOPO single treatment. Conclusion: Carvacrol had enhanced TOPO cytotoxicity and cellular uptake in HeLa and HCT116 cancer cells but might cause TOPO resistance in MCF-7, HepG2, SKOV3 and A549 cells.

scholarly journals In Vivo Targeting of Xenografted Human Cancer Cells with Functionalized Fluorescent Silica Nanoparticles in Zebrafish

10.3791/61187 ◽  
2020 ◽  
Author(s):  
Xiaodan Qin ◽  
Fabrice F. J. Laroche ◽  
Saquib Ahmed M. A. Peerzade ◽  
Andrew Lam ◽  
Igor Sokolov ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Fluorescent Silica Nanoparticles

Indirubin derivatives inhibit SFKs/Stat signaling associated with apoptosis in human cancer cells

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
S Nam ◽  
R Buettner ◽  
X Liu ◽  
J Turkson ◽  
D Kim ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Stat Signaling
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