scholarly journals Extracellular polymeric substance‐mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment

2020 ◽  
Vol 17 (12) ◽  
pp. 2000108
Author(s):  
Nid'a H. Alshraiedeh ◽  
Stephen A. Kelly ◽  
Thomas P. Thompson ◽  
Padrig B. Flynn ◽  
Michael M. Tunney ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Plasma Treatment ◽  
Atmospheric Pressure ◽  
Extracellular Polymeric Substance ◽  
Nonthermal Plasma ◽  
Polymeric Substance

scholarly journals Inactivation of Pseudomonas aeruginosa Biofilms by 405-Nanometer-Light-Emitting Diode Illumination

2020 ◽  
Vol 86 (10) ◽  
Author(s):  
Yanpeng Yang ◽  
Sheng Ma ◽  
Yawen Xie ◽  
Muxue Wang ◽  
Ting Cai ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stainless Steel ◽  
Extracellular Polymeric Substance ◽  
Biofilm Formation ◽  
Light Emitting Diode ◽  
High Morbidity ◽  
Content Type ◽  
Light Emitting ◽  
Polymeric Substance ◽  
Biofilm Structure

ABSTRACT Biofilm formation by Pseudomonas aeruginosa contributes to its survival on surfaces and represents a major clinical threat because of the increased tolerance of biofilms to disinfecting agents. This study aimed to investigate the efficacy of 405-nm light-emitting diode (LED) illumination in eliminating P. aeruginosa biofilms formed on stainless steel coupons under different temperatures. Time-dependent killing assays using planktonic and biofilm cells were used to determine the antimicrobial and antibiofilm activities of LED illumination. We also evaluated the effects of LED illumination on the disinfectant susceptibility, biofilm structure, extracellular polymeric substance (EPS) structure and composition, and biofilm-related gene expression of P. aeruginosa biofilm cells. Results showed that the abundance of planktonic P. aeruginosa cells was reduced by 0.88, 0.53, and 0.85 log CFU/ml following LED treatment for 2 h compared with untreated controls at 4, 10, and 25°C, respectively. For cells in biofilms, significant reductions (1.73, 1.59, and 1.68 log CFU/cm2) were observed following LED illumination for 2 h at 4, 10, and 25°C, respectively. Moreover, illuminated P. aeruginosa biofilm cells were more sensitive to benzalkonium chloride or chlorhexidine than untreated cells. Scanning electron microscopy and confocal laser scanning microscopic observation indicated that both the biofilm structure and EPS structure were disrupted by LED illumination. Further, reverse transcription-quantitative PCR revealed that LED illumination downregulated the transcription of several genes associated with biofilm formation. These findings suggest that LED illumination has the potential to be developed as an alternative method for prevention and control of P. aeruginosa biofilm contamination. IMPORTANCE Pseudomonas aeruginosa can form biofilms on medical implants, industrial equipment, and domestic surfaces, contributing to high morbidity and mortality rates. This study examined the antibiofilm activity of 405-nm light-emitting diode (LED) illumination against mature biofilms formed on stainless steel coupons. We found that the disinfectant susceptibility, biofilm structure, and extracellular polymeric substance structure and composition were disrupted by LED illumination. We then investigated the transcription of several critical P. aeruginosa biofilm-related genes and analyzed the effect of illumination temperature on the above characteristics. Our results confirmed that LED illumination could be developed into an effective and safe method to counter P. aeruginosa biofilm contamination. Further research will be focused on the efficacy and application of LED illumination for elimination of complicated biofilms in the environment.

scholarly journals Enhanced Wetting and Adhesive Properties by Atmospheric Pressure Plasma Surface Treatment Methods and Investigation Processes on the Influencing Parameters on HIPS Polymer

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 901
Author(s):  
Miklós Berczeli ◽  
Zoltán Weltsch
Keyword(s):  
Surface Treatment ◽  
Plasma Treatment ◽  
Atmospheric Pressure ◽  
High Impact ◽  
Plasma Parameters ◽  
Plasma Surface ◽  
Wetting Properties ◽  
High Impact Polystyrene ◽  
Process Modification ◽  
Plasma Surface Treatment

The development of bonding technology and coating technologies require the use of modern materials and topologies for the demanding effect and modification of their wetting properties. For the industry, a process modification process that can be integrated into a process is the atmospheric pressure of air operation plasma surface treatment. This can be classified and evaluated based on the wettability, which has a significant impact on the adhesive force. The aim is to improve the wetting properties and to find the relationship between plasma treatment parameters, wetting, and adhesion. High Impact PolyStyrene (HIPS) was used as an experimental material, and then the plasma treatment can be treated with various adjustable parameters. The effect of plasma parameters on surface roughness, wetting contact angle, and using Fowkes theory of the surface energy have been investigated. Seven different plasma jet treatment distances were tested, combined with 5 scan speeds. Samples with the best plasma parameters were prepared from 25 mm × 25 mm overlapping adhesive joints using acrylic/cyanoacrylate. The possibility of creating a completely hydrophilic surface was achieved, where the untreated wetting edge angle decreased from 88.2° to 0° for distilled water and from 62.7° to 0° in the case of ethylene glycol. The bonding strength of High Impact PolyStyrene was increased by plasma treatment by 297%.

The effect of atmospheric pressure plasma treatment on wetting and absorbance properties of cotton fabric

2021 ◽  
Author(s):  
Thisara Sandanuwan ◽  
Nayanathara Hendeniya ◽  
D.A.S. Amarasinghe ◽  
Dinesh Attygalle ◽  
Sampath Weragoda
Keyword(s):  
Plasma Treatment ◽  
Cotton Fabric ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma

The extracellular polymeric substance of the green alga Penium margaritaceum and its role in biofilm formation

Biofilms ◽  
2005 ◽  
Vol 2 (2) ◽  
pp. 129-144 ◽  
Author(s):  
D. S. Domozych ◽  
S. Kort ◽  
S. Benton ◽  
T. Yu
Keyword(s):  
New York ◽  
Extracellular Polymeric Substance ◽  
Biofilm Formation ◽  
Glucuronic Acid ◽  
New York State ◽  
Immunofluorescence Analysis ◽  
Capsule Formation ◽  
Polymeric Substance ◽  
Cell Substrate ◽  
Initial Adhesion

The desmid Penium margaritaceum is a common resident of biofilms of shallow Adirondack wetlands in New York State, USA. It was isolated and grown in the laboratory where it readily formed biofilms and produced large amounts of extracellular polymeric substance (EPS). The EPS was separated into two fractions: an EPS gel and soluble EPS. Both fractions were rich in xylose, fucose and glucuronic acid. The EPS gels contained large amounts of 3-linked, 4-linked and 3,4-linked fucose, 3,4-linked glucuronic acid and terminal xylose linkages. The EPS gel consisted of a fibrillar matrix that linked cells and cell substrate together. Immunofluorescence analysis using an anti-EPS antibody revealed that EPS secretion occurs in several different modes, which contributes to initial adhesion, capsule formation and gliding.

scholarly journals In-package atmospheric pressure cold plasma treatment of cherry tomatoes

2014 ◽  
Vol 118 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Nrusimha Nath Misra ◽  
Kevin M. Keener ◽  
Paula Bourke ◽  
Jean-Paul Mosnier ◽  
Patrick J. Cullen
Keyword(s):  
Plasma Treatment ◽  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Cherry Tomatoes

Using atmospheric pressure plasma treatment for treating grey cotton fabric

2014 ◽  
Vol 102 ◽  
pp. 167-173 ◽  
Author(s):  
Chi-Wai Kan ◽  
Chui-Fung Lam ◽  
Chee-Kooi Chan ◽  
Sun-Pui Ng
Keyword(s):  
Plasma Treatment ◽  
Cotton Fabric ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma
Sign in / Sign up

Export Citation Format

Share Document