Experimental Study of the Plasma Polymerization of Ethyl Lactate

2013 ◽  
Vol 10 (11) ◽  
pp. 999-1009 ◽  
Author(s):  
Sylvie Ligot ◽  
Fabian Renaux ◽  
Laurent Denis ◽  
Damien Cossement ◽  
Nicolas Nuns ◽  
...  
2014 ◽  
Vol 12 (5) ◽  
pp. 405-415 ◽  
Author(s):  
Sylvie Ligot ◽  
Maxime Guillaume ◽  
Patrice Raynaud ◽  
Damien Thiry ◽  
Vincent Lemaur ◽  
...  

2009 ◽  
Vol 7 (2) ◽  
pp. 172-181 ◽  
Author(s):  
Laurent Denis ◽  
Philippe Marsal ◽  
Yoann Olivier ◽  
Thomas Godfroid ◽  
Roberto Lazzaroni ◽  
...  

2017 ◽  
Vol 50 (47) ◽  
pp. 475205 ◽  
Author(s):  
Morgane Laurent ◽  
Edouard Desjardins ◽  
Maximilian Meichelboeck ◽  
Nicolas Naudé ◽  
Luc Stafford ◽  
...  

Author(s):  
A. Tanaka ◽  
M. Yamaguchi ◽  
T. Hirano

The plasma polymerization replica method and its apparatus have been devised by Tanaka (1-3). We have published several reports on its application: surface replicas of biological and inorganic specimens, replicas of freeze-fractured tissues and metal-extraction replicas with immunocytochemical markers.The apparatus for plasma polymerization consists of a high voltage power supply, a vacuum chamber containing a hydrocarbon gas (naphthalene, methane, ethylene), and electrodes of an anode disk and a cathode of the specimen base. The surface replication by plasma polymerization in negative glow phase on the cathode was carried out by gassing at 0.05-0.1 Torr and glow discharging at 1.5-3 kV D.C. Ionized hydrocarbon molecules diffused into complex surface configurations and deposited as a three-dimensionally polymerized film of 1050 nm in thickness.The resulting film on the complex surface had uniform thickness and showed no granular texture. Since the film was chemically inert, resistant to heat and mecanically strong, it could be treated with almost any organic or inorganic solvents.


Author(s):  
Hirano T. ◽  
M. Yamaguchi ◽  
M. Hayashi ◽  
Y. Sekiguchi ◽  
A. Tanaka

A plasma polymerization film replica method is a new high resolution replica technique devised by Tanaka et al. in 1978. It has been developed for investigation of the three dimensional ultrastructure in biological or nonbiological specimens with the transmission electron microscope. This method is based on direct observation of the single-stage replica film, which was obtained by directly coating on the specimen surface. A plasma polymerization film was deposited by gaseous hydrocarbon monomer in a glow discharge.The present study further developed the freeze fracture method by means of a plasma polymerization film produces a three dimensional replica of chemically untreated cells and provides a clear evidence of fine structure of the yeast plasma membrane, especially the dynamic aspect of the structure of invagination (Figure 1).


Author(s):  
Norio Baba ◽  
Norihiko Ichise ◽  
Syunya Watanabe

The tilted beam illumination method is used to improve the resolution comparing with the axial illumination mode. Using this advantage, a restoration method of several tilted beam images covering the full azimuthal range was proposed by Saxton, and experimentally examined. To make this technique more reliable it seems that some practical problems still remain. In this report the restoration was attempted and the problems were considered. In our study, four problems were pointed out for the experiment of the restoration. (1) Accurate beam tilt adjustment to fit the incident beam to the coma-free axis for the symmetrical beam tilting over the full azimuthal range. (2) Accurate measurements of the optical parameters which are necessary to design the restoration filter. Even if the spherical aberration coefficient Cs is known with accuracy and the axial astigmatism is sufficiently compensated, at least the defocus value must be measured. (3) Accurate alignment of the tilt-azimuth series images.


1962 ◽  
Vol 5 (4) ◽  
pp. 387-394 ◽  
Author(s):  
Bruce Quarrington ◽  
Jerome Conway ◽  
Nathan Siegel
Keyword(s):  

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