Effect of Polymer Ionization on the Interaction with DNA in Nonviral Gene Delivery Systems

2003 ◽  
Vol 4 (3) ◽  
pp. 683-690 ◽  
Author(s):  
Uracha Rungsardthong ◽  
Touraj Ehtezazi ◽  
Lindsey Bailey ◽  
Steven P. Armes ◽  
Martin C. Garnett ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Nonviral Gene Delivery

scholarly journals Polymeric Nonviral Gene Delivery Systems for Cancer Immunotherapy

2020 ◽  
Vol 3 (6) ◽  
pp. 1900213 ◽  
Author(s):  
Lingjie Ke ◽  
Pingqiang Cai ◽  
Yun‐Long Wu ◽  
Xiaodong Chen
Keyword(s):  
Gene Delivery ◽  
Cancer Immunotherapy ◽  
Delivery Systems ◽  
Nonviral Gene Delivery

scholarly journals Enhanced PRL-1 expression in placenta-derived mesenchymal stem cells accelerates hepatic function via mitochondrial dynamics in a cirrhotic rat model

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jae Yeon Kim ◽  
Jong Ho Choi ◽  
Ji Hye Jun ◽  
Sohae Park ◽  
Jieun Jung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Cell Therapy ◽  
Delivery Systems ◽  
Nonviral Gene Delivery ◽  
Early Gene ◽  
Next Generation ◽  
Atp Production ◽  
Mitochondrial Functions

Abstract Background Placenta-derived mesenchymal stem cells (PD-MSCs) have been highlighted as an alternative cell therapy agent that has become a next-generation stem cell treatment. Phosphatase of regenerating liver-1 (PRL-1), an immediate early gene, plays a critical role during liver regeneration. Here, we generated enhanced PRL-1 in PD-MSCs (PD-MSCsPRL-1, PRL-1+) using lentiviral and nonviral gene delivery systems and investigated mitochondrial functions by PD-MSCPRL-1 transplantation for hepatic functions in a rat bile duct ligation (BDL) model. Methods PD-MSCsPRL-1 were generated by lentiviral and nonviral AMAXA gene delivery systems and analyzed for their characteristics and mitochondrial metabolic functions. Liver cirrhosis was induced in Sprague-Dawley (SD) rats using common BDL for 10 days. PKH67+ naïve and PD-MSCsPRL-1 using a nonviral sysyem (2 × 106 cells/animal) were intravenously administered into cirrhotic rats. The animals were sacrificed at 1, 2, 3, and 5 weeks after transplantation and engraftment of stem cells, and histopathological analysis and hepatic mitochondrial functions were performed. Results PD-MSCsPRL-1 were successfully generated using lentiviral and nonviral AMAXA systems and maintained characteristics similar to those of naïve cells. Compared with naïve cells, PD-MSCsPRL-1 improved respirational metabolic states of mitochondria. In particular, mitochondria in PD-MSCsPRL-1 generated by the nonviral AMAXA system showed a significant increase in the respirational metabolic state, including ATP production and mitochondrial biogenesis (*p < 0.05). Furthermore, transplantation of PD-MSCsPRL-1 using a nonviral AMAXA system promoted engraftment into injured target liver tissues of a rat BDL cirrhotic model and enhanced the metabolism of mitochondria via increased mtDNA and ATP production, thereby improving therapeutic efficacy. Conclusions Our findings will further our understanding of the therapeutic mechanism of enhanced MSCs and provide useful data for the development of next-generation MSC-based cell therapy and therapeutic strategies for regenerative medicine in liver disease.

scholarly journals Triazine Dendrimers as Nonviral Gene Delivery Systems: Effects of Molecular Structure on Biological Activity

2009 ◽  
Vol 20 (9) ◽  
pp. 1799-1806 ◽  
Author(s):  
Olivia M. Merkel ◽  
Meredith A. Mintzer ◽  
Johannes Sitterberg ◽  
Udo Bakowsky ◽  
Eric E. Simanek ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Biological Activity ◽  
Gene Delivery ◽  
Delivery Systems ◽  
Nonviral Gene Delivery

Gene Therapy: Recent Advances and Applications in Dermatology

1996 ◽  
Vol 1 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Michael J. Hope
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Skin Diseases ◽  
Ex Vivo ◽  
Delivery Systems ◽  
Nonviral Gene Delivery ◽  
Regulatory Sequences ◽  
Advantages And Disadvantages ◽  
Technical Advances ◽  
Near Future

Background: Gene therapy is an innovative and exciting new branch of medicine. Despite the fact that a human disease has yet to be cured using this therapeutic approach, numerous clinical trials are taking place around the world based on encouraging preclinical data. Objective: The aim of this review is to bring the reader up to date with this rapidly advancing field and to highlight the technical advances that must occur before gene therapy will become common practice in dermatology. Methods: The current level of gene delivery technology restricts the applications. The advantages and disadvantages of viral and nonviral gene delivery systems are discussed. Results: Considerable advances are being made in the areas of cancer immunotherapy and vaccines. Of particular importance to the treatment of skin diseases will be the isolation and ex vivo manipulation of epidermal stem cells, the development of skin-specific regulatory sequences for gene expression, and the formulation of gene delivery systems suitable for systemic administration. Conclusions: In general, skin and keratinocytes are considered to be good targets for gene transfer applications, and several diseases have been identified as potential candidates for treatment in the near future.

Biodegradable and Nontoxic Nanogels as Nonviral Gene Delivery Systems

2012 ◽  
Vol 23 (9) ◽  
pp. 1925-1933 ◽  
Author(s):  
Rajesh Sunasee ◽  
Phanphen Wattanaarsakit ◽  
Marya Ahmed ◽  
Farahnaz Begum Lollmahomed ◽  
Ravin Narain
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Nonviral Gene Delivery
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