Insight into Salivary Gland Proteomes of Two Polyphagous Stink Bugs: Nezara viridula L. and Halyomorpha halys Stål

PROTEOMICS ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1800436 ◽  
Author(s):  
Laurent Serteyn ◽  
Frederic Francis
Keyword(s):  
Salivary Gland ◽  
Nezara Viridula ◽  
Halyomorpha Halys ◽  
Stink Bugs ◽  
Insight Into

scholarly journals Pentatomids associated with blackberry

2014 ◽  
Vol 38 (3) ◽  
pp. 256-261
Author(s):  
Mauricio Paulo Batistella Pasini ◽  
Alessandro Dal'Col Lúcio
Keyword(s):  
Rio Grande ◽  
Nezara Viridula ◽  
Brown Spot ◽  
Stink Bug ◽  
Stink Bugs ◽  
Rio Grande Do Sul ◽  
Dark Brown Spot ◽  
Euschistus Heros ◽  
Santa Maria ◽  
Piezodorus Guildinii

Information concerning the presence of stink bugs in blackberry (Rubus spp.) in Brazil is sparse. This study aimed to identify the stink bug species associated with blackberry, to establish the daily dynamics and evaluate the fruits damage. The experiment was conducted in Santa Maria, Rio Grande do Sul State, Brazil, in blackberry orchard. Presence and evaluations of stink bugs were done weekly through visual and sweeping samplings at different day times. Five species of pentatomids were identified: Piezodorus guildinii, Nezara viridula, Euschistus heros, Dichelops furcatus and Edessa meditabunda. The bugs attack the drupelets producing a dark brown spot and wrinkled berries.

scholarly journals Trophic Evolution of Southern Green Stink Bugs (Nezara viridula l.) in Western Part of Romania

2015 ◽  
Vol 72 (2) ◽  
Author(s):  
Ioana Grozea ◽  
Ana Maria Virteiu ◽  
Ramona Stef ◽  
Alin Carabet ◽  
Levente Molnar ◽  
...  
Keyword(s):  
Nezara Viridula ◽  
Stink Bugs

Effect of Trap Color and Residual Attraction of a Pheromone Lure for Monitoring Stink Bugs (Hemiptera: Pentatomidae)

2020 ◽  
Vol 55 (4) ◽  
pp. 437-447
Author(s):  
Ted E. Cottrell ◽  
Rammohan R. Balusu ◽  
Edgar Vinson ◽  
Bryan Wilkins ◽  
Henry Y. Fadamiro ◽  
...  
Keyword(s):  
Native Species ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Stink Bugs ◽  
Euschistus Servus ◽  
Trap Color ◽  
Residual Efficacy ◽  
Conducting Research ◽  
Multiple Species ◽  
Research Studies

Abstract Stink bugs (Hemiptera: Pentatomidae) are commonly monitored using pyramid traps baited with a pheromone. Initially, the pyramid traps were painted yellow and predominantly used to monitor native stink bug species. However, research studies involving the exotic Halyomorpha halys Stål (Hemiptera: Pentatomidae) now use pyramid traps that are black, not yellow. As H. halys moves across the southeastern United States, the use of a single trap, yellow or black, for monitoring and conducting research studies would be beneficial. Our objective was to compare black and yellow pyramid traps baited with a lure to determine if one was superior for trapping herbivorous stink bugs. This study was conducted at four locations, three in Alabama and one in Georgia, over 2 yr. Additionally, residual efficacy of the lure was measured via trap capture over 1-mo intervals. Our results showed that only when native stink bug species were combined, and only in 1 yr, were captures significantly affected by trap color. Capture of the exotic H. halys and the most abundant native species, Euschistus servus (Say), was not significantly affected by trap color. Trap capture was significantly affected by how long a lure was in a trap. The data from this study suggests that when traps are used in conjunction with a pheromone to monitor multiple species of adult stink bugs, especially native species, the yellow pyramid trap is favored.

scholarly journals Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

2021 ◽  
Vol 12 ◽  
Author(s):  
Zehua Wang ◽  
Fan Yang ◽  
Ang Sun ◽  
Shuang Shan ◽  
Yongjun Zhang ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Functional Characterization ◽  
Insect Species ◽  
Pcr Analysis ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Stink Bugs ◽  
Binding Assays ◽  
Chemosensory Protein ◽  
Reverse Transcription Pcr

Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys, a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs (HhalCSP15) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that HhalCSP15 was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone (Ki=11.9±0.6μM), cis-3-hexen-1-yl benzoate (Ki=10.5±0.4μM), and methyl (2E,4E,6Z)-decatrienoate (EEZ-MDT; Ki=9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG). EEZ-MDT is particularly interesting, as it serves as a kairomone when H. halys forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in H. halys, and extend the olfactory function of CSPs in stink bugs.

scholarly journals A transcriptomic and proteomic atlas of expression in the Nezara viridula (Heteroptera: Pentatomidae) midgut suggests the compartmentalization of xenobiotic metabolism and nutrient digestion

2019 ◽  
Author(s):  
Shane Denecke ◽  
Panagiotis Ioannidis ◽  
Benjamin Buer ◽  
Aris Ilias ◽  
Vassilis Douris ◽  
...  
Keyword(s):  
Initial Step ◽  
Xenobiotic Metabolism ◽  
The Body ◽  
Nezara Viridula ◽  
Stink Bug ◽  
Stink Bugs ◽  
Molecular Physiology ◽  
Southern Green Stink Bug ◽  
Green Stink Bug ◽  
Species Specific

Abstract Background Stink bugs are an emerging threat to crop security in many parts of the globe, but there are few genetic resources available to study their physiology at a molecular level. This is especially true for tissues such as the midgut, which forms the barrier between ingested material and the inside of the body. Results Here, we focus on the midgut of the southern green stink bug Nezara viridula and use both transcriptomic and proteomic approaches to create an atlas of expression along the four compartments of the anterior-posterior axis. Estimates of the transcriptome completeness were high, which led us to compare our predicted gene set to other related stink bugs and Hemiptera, finding a high number of species-specific genes in N. viridula. To understand midgut function, gene ontology and gene family enrichment analyses were performed for the most highly expressed and specific genes in each midgut compartment. These data suggested a role for the anterior midgut (regions M1-M3) in digestion and xenobiotic metabolism, while the most posterior compartment (M4) was enriched in transmembrane proteins. A more detailed characterization of these findings was undertaken by identifying individual members of the cytochrome P450 superfamily and nutrient transporters thought to absorb amino acids or sugars. Conclusions These findings represent an initial step to understand the compartmentalization and physiology of the N. viridula midgut at a genetic level. Future studies will be able to build on this work and explore the molecular physiology of the stink bug midgut.

scholarly journals An insight into the transcriptome and proteome of the salivary gland of the stable fly, Stomoxys calcitrans

2009 ◽  
Vol 39 (9) ◽  
pp. 607-614 ◽  
Author(s):  
Xuyong Wang ◽  
José M.C. Ribeiro ◽  
Alberto B. Broce ◽  
Melinda J. Wilkerson ◽  
Michael R. Kanost
Keyword(s):  
Salivary Gland ◽  
Stomoxys Calcitrans ◽  
Stable Fly ◽  
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