scholarly journals Inside Front Cover: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma

PROTEOMICS ◽  
2016 ◽  
Vol 16 (24) ◽  
pp. NA-NA ◽  
Author(s):  
Pamela Norton ◽  
Mary Ann Comunale ◽  
Harmin Herrera ◽  
Mengjun Wang ◽  
Josef Houser ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Front Cover ◽  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

Epithelial repair is inhibited by an α1,6-fucose binding lectin

2007 ◽  
Vol 292 (2) ◽  
pp. L462-L468 ◽  
Author(s):  
Elizabeth C. Adam ◽  
Stephen T. Holgate ◽  
Peter M. Lackie
Keyword(s):  
Binding Sites ◽  
Repair Process ◽  
Airway Epithelial ◽  
Free Medium ◽  
Epithelial Repair ◽  
Aleuria Aurantia Lectin ◽  
Cell Substrate ◽  
Binding Lectin ◽  
Aleuria Aurantia ◽  
Insight Into

The effective repair of damage to the airway epithelium is essential to maintain the ability to exclude airborne particulates and protect against potential pathogens. Carbohydrates on the cell surface have an important role in cell-cell and cell substrate interactions. Using a model of repair with airway epithelial-derived cells of the 16HBE 14o− cell line, we have examined the effect of the Aleuria aurantia lectin (AAL), which binds very selectively to α1,6-linked fucose residues. Addition of unconjugated or FITC-labeled AAL reduced the rate of epithelial repair to approximately one-third of control values as measured by image analysis while cell viability was maintained. Pulse labeling with AAL-FITC for 30 min followed by incubation in AAL-free medium caused similar inhibition of repair but could be reversed by addition of fucose up to 7 h after AAL removal. By confocal microscopy, AAL binding was found to be on the apical, but not basolateral, surfaces of cells, and internalization of the labeled lectin was seen. Preincubation of the lectin with fucose prevented this effect. Ulex europeaus I lectin, which is also fucose specific, resulted in similar binding to the cells and internalization, but it did not affect the speed of the repair process. We conclude that α1,6-fucose binding sites play an important role in epithelial repair. Better understanding of this process will provide a deeper insight into the crucial mechanisms of epithelial repair.

scholarly journals Elucidating the selectivity of recombinant forms of Aleuria aurantia lectin using weak affinity chromatography

2012 ◽  
Vol 885-886 ◽  
pp. 66-72 ◽  
Author(s):  
Maria Bergström ◽  
Eva Åström ◽  
Peter Påhlsson ◽  
Sten Ohlson
Keyword(s):  
Affinity Chromatography ◽  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

scholarly journals Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin

Glycobiology ◽  
2010 ◽  
Vol 21 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Johan Olausson ◽  
Eva Åström ◽  
Bengt-Harald Jonsson ◽  
Lena A E Tibell ◽  
Peter Påhlsson
Keyword(s):  
Single Site ◽  
Monomeric Form ◽  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

scholarly journals Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0189375 ◽  
Author(s):  
Josef Houser ◽  
Stanislav Kozmon ◽  
Deepti Mishra ◽  
Sushil K. Mishra ◽  
Patrick R. Romano ◽  
...  
Keyword(s):  
Carbohydrate Binding ◽  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

Front Cover: Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes

PROTEOMICS ◽  
2017 ◽  
Vol 17 (13-14) ◽  
pp. 1770111 ◽  
Author(s):  
Jing Zhang ◽  
Shaohua Lu ◽  
Ye Zhou ◽  
Kun Meng ◽  
Zhipeng Chen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Sugar Metabolism ◽  
Carcinoma Cells ◽  
Regulatory Proteins ◽  
Front Cover ◽  
Hepatocellular Carcinoma Cells

Improved cancer specificity in PSA assay using Aleuria aurantia lectin coated Eu-nanoparticles for detection

2017 ◽  
Vol 50 (1-2) ◽  
pp. 54-61 ◽  
Author(s):  
Henna Kekki ◽  
Mari Peltola ◽  
Sandra van Vliet ◽  
Chris Bangma ◽  
Yvette van Kooyk ◽  
...  
Keyword(s):  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

Detection of a high affinity binding site in recombinant Aleuria aurantia lectin

2008 ◽  
Vol 25 (8) ◽  
pp. 753-762 ◽  
Author(s):  
Johan Olausson ◽  
Lena Tibell ◽  
Bengt-Harald Jonsson ◽  
Peter Påhlsson
Keyword(s):  
Binding Site ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity ◽  
Aleuria Aurantia Lectin ◽  
High Affinity Binding Site ◽  
Aleuria Aurantia

scholarly journals Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin.

1985 ◽  
Vol 260 (8) ◽  
pp. 4688-4693 ◽  
Author(s):  
K Yamashita ◽  
N Kochibe ◽  
T Ohkura ◽  
I Ueda ◽  
A Kobata
Keyword(s):  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia
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