Lentil lectin-unrelated reactivity of Aleuria aurantia lectin in affinity electrophoresis of alpha-fetoprotein.

1991 ◽  
Vol 35 (4) ◽  
pp. 265-269
Author(s):  
Kazuhisa Taketa ◽  
Hiroko Taga ◽  
Hidematsu Hirai
Keyword(s):  
Alpha Fetoprotein ◽  
Affinity Electrophoresis ◽  
Aleuria Aurantia Lectin ◽  
Lentil Lectin ◽  
Aleuria Aurantia

Clinical Evaluation of Lentil Lectin-Reactive Alpha-Fetoprotein-L3 in Histology-Proven Hepatocellular Carcinoma

2001 ◽  
Vol 16 (2) ◽  
pp. 105-111 ◽  
Author(s):  
V.V. Khien ◽  
H.V. Mao ◽  
T.T. Chinh ◽  
P.T. Ha ◽  
M.H. Bang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Diseases ◽  
Alpha Fetoprotein ◽  
Chronic Liver ◽  
Chronic Liver Diseases ◽  
Antibody Affinity ◽  
Lectin Affinity ◽  
Affinity Electrophoresis ◽  
Lentil Lectin ◽  
The Mean

Introduction Serum alpha-fetoprotein (AFP) is a useful marker of hepatocellular carcinoma (HCC), although the serum AFP concentration is also increased in patients with chronic liver diseases (CLD). The analysis of AFP glycoforms has been known to be of diagnostic value. We applied the lectin-affinity electrophoresis and antibody-affinity blotting techniques to HCC patients in Vietnam in order to better understand the role of lentil lectin-affinity AFP-L3 in the diagnosis and differential diagnosis of HCC, and its relationship with the biological characteristics of HCC. Methods Lens culinaris agglutinin-reactive AFP (AFP-L3) was measured in 65 patients with histologically proven HCC and 25 patients with CLD. All patients had serum AFP levels above 54 ng/mL. AFP-L3 levels were determined by lectin affinity electrophoresis coupled with antibody-affinity blotting. The diagnosis of HCC was confirmed histologically by ultrasound-guided biopsy. Results The mean value of AFP-L3 in the HCC patients was 49.6 ± 21.6%, which was significantly higher (p<0.001) than that in the 25 CLD patients (10.7 ± 4.3%). When the cutoff level for AFP-L3 was set at 15% (mean ± SD), the sensitivity was 96.9%, the specificity 92.0% and the accuracy 95.5% in the 65 HCC patients. There was no clear correlation between serum AFP level and AFP-L3 percentage (r=0.16). There was no correlation between AFP-L3 and the maximum diameter of HCC nodules (r=0.05). However, the mean AFP-L3 value was higher in moderately or poorly differentiated HCC than in well differentiated tumors (p<0.001). Conclusions AFP-L3 is potentially a clinically useful marker for the differentiation of increased AFP levels in hepatocellular carcinoma and chronic liver diseases. The AFP-L3 percentage is closely related to HCC differentiation. We consider the analysis of AFP-L3 a useful adjunct in the diagnosis of HCC.

Lentil-lectin-reactive alpha-fetoprotein in the differential diagnosis of benign and malignant liver disease.

1986 ◽  
Vol 32 (11) ◽  
pp. 2083-2084 ◽  
Author(s):  
P K Buamah ◽  
R Harris ◽  
O F James ◽  
A W Skillen
Keyword(s):  
Differential Diagnosis ◽  
Liver Disease ◽  
Affinity Chromatography ◽  
Simple Technique ◽  
Alpha Fetoprotein ◽  
Lentil Lectin ◽  
Serum Alpha Fetoprotein ◽  
Malignant Liver Disease ◽  
Malignant Liver ◽  
Sepharose 4B

Abstract Affinity chromatography of serum on lentil lectin bound to Sepharose 4B has been used to identify different forms of alpha-fetoprotein in serum of patients with liver disease. We studied eight patients with non-malignant liver disease, finding that 7-15% of the serum alpha-fetoprotein bound to the lectin. In contrast, for 15 patients with malignant liver disease, 25-83% of the serum alpha-fetoprotein bound to the lectin. Evidently, this simple technique can be used effectively to differentiate these two conditions.

Epithelial repair is inhibited by an α1,6-fucose binding lectin

2007 ◽  
Vol 292 (2) ◽  
pp. L462-L468 ◽  
Author(s):  
Elizabeth C. Adam ◽  
Stephen T. Holgate ◽  
Peter M. Lackie
Keyword(s):  
Binding Sites ◽  
Repair Process ◽  
Airway Epithelial ◽  
Free Medium ◽  
Epithelial Repair ◽  
Aleuria Aurantia Lectin ◽  
Cell Substrate ◽  
Binding Lectin ◽  
Aleuria Aurantia ◽  
Insight Into

The effective repair of damage to the airway epithelium is essential to maintain the ability to exclude airborne particulates and protect against potential pathogens. Carbohydrates on the cell surface have an important role in cell-cell and cell substrate interactions. Using a model of repair with airway epithelial-derived cells of the 16HBE 14o− cell line, we have examined the effect of the Aleuria aurantia lectin (AAL), which binds very selectively to α1,6-linked fucose residues. Addition of unconjugated or FITC-labeled AAL reduced the rate of epithelial repair to approximately one-third of control values as measured by image analysis while cell viability was maintained. Pulse labeling with AAL-FITC for 30 min followed by incubation in AAL-free medium caused similar inhibition of repair but could be reversed by addition of fucose up to 7 h after AAL removal. By confocal microscopy, AAL binding was found to be on the apical, but not basolateral, surfaces of cells, and internalization of the labeled lectin was seen. Preincubation of the lectin with fucose prevented this effect. Ulex europeaus I lectin, which is also fucose specific, resulted in similar binding to the cells and internalization, but it did not affect the speed of the repair process. We conclude that α1,6-fucose binding sites play an important role in epithelial repair. Better understanding of this process will provide a deeper insight into the crucial mechanisms of epithelial repair.

scholarly journals Elucidating the selectivity of recombinant forms of Aleuria aurantia lectin using weak affinity chromatography

2012 ◽  
Vol 885-886 ◽  
pp. 66-72 ◽  
Author(s):  
Maria Bergström ◽  
Eva Åström ◽  
Peter Påhlsson ◽  
Sten Ohlson
Keyword(s):  
Affinity Chromatography ◽  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

scholarly journals Diagnosis of hepatocellular carcinoma by concanavalin a affinity electrophoresis of serum alpha-fetoprotein

Cancer ◽  
1995 ◽  
Vol 76 (7) ◽  
pp. 1139-1144 ◽  
Author(s):  
Satoshi Saitoh ◽  
Kenji Ikeda ◽  
Isao Koida ◽  
Yoshiyuki Suzuki ◽  
Masahiro Kobayashi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Concanavalin A ◽  
Alpha Fetoprotein ◽  
Affinity Electrophoresis ◽  
Serum Alpha Fetoprotein

scholarly journals Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin

Glycobiology ◽  
2010 ◽  
Vol 21 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Johan Olausson ◽  
Eva Åström ◽  
Bengt-Harald Jonsson ◽  
Lena A E Tibell ◽  
Peter Påhlsson
Keyword(s):  
Single Site ◽  
Monomeric Form ◽  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

scholarly journals Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0189375 ◽  
Author(s):  
Josef Houser ◽  
Stanislav Kozmon ◽  
Deepti Mishra ◽  
Sushil K. Mishra ◽  
Patrick R. Romano ◽  
...  
Keyword(s):  
Carbohydrate Binding ◽  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia

Establishment of assay kits for the determination of microheterogeneities of alpha-fetoprotein using lectin-affinity electrophoresis

1993 ◽  
Vol 214 (1) ◽  
pp. 3-12 ◽  
Author(s):  
Kayoko Shimizu ◽  
Tomoko Taniichi ◽  
Shinji Satomura ◽  
Shuji Matsuura ◽  
Hiroko Taga ◽  
...  
Keyword(s):  
Alpha Fetoprotein ◽  
Lectin Affinity ◽  
Affinity Electrophoresis

Improved cancer specificity in PSA assay using Aleuria aurantia lectin coated Eu-nanoparticles for detection

2017 ◽  
Vol 50 (1-2) ◽  
pp. 54-61 ◽  
Author(s):  
Henna Kekki ◽  
Mari Peltola ◽  
Sandra van Vliet ◽  
Chris Bangma ◽  
Yvette van Kooyk ◽  
...  
Keyword(s):  
Aleuria Aurantia Lectin ◽  
Aleuria Aurantia
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