Proteomic analysis of pear (Pyrus pyrifolia) ripening process provides new evidence for the sugar/acid metabolism difference between core and mesocarp

PROTEOMICS ◽  
2016 ◽  
Vol 16 (23) ◽  
pp. 3025-3041 ◽  
Author(s):  
Zhen Gao ◽  
Chengjun Zhang ◽  
Meng Luo ◽  
Yusen Wu ◽  
Shuyan Duan ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Acid Metabolism ◽  
Pyrus Pyrifolia ◽  
Ripening Process ◽  
New Evidence

scholarly journals Comparative Proteomic Analysis of Wild-Type Physcomitrella patens and an OPDA-Deficient Physcomitrella patens Mutant with Disrupted PpAOS1 and PpAOS2 Genes after Wounding

2020 ◽  
Author(s):  
Weifeng Luo ◽  
Setsuko Komatsu ◽  
Tatsuya Abe ◽  
Hideyuki Matsuura ◽  
Kosaku Talahashi
Keyword(s):  
Protein Synthesis ◽  
Proteomic Analysis ◽  
Vascular Plants ◽  
Acid Metabolism ◽  
Physcomitrella Patens ◽  
Energy Utilization ◽  
Wild Type Plant ◽  
Wild Type ◽  
In The Wild ◽  
Energy Synthesis

Wounding is a serious environmental stress in plants. Oxylipins such as jasmonic acid play an important role in defense against wounding. Mechanisms to adapt to wounding have been investigated in vascular plants; however, those mechanisms in nonvascular plants remain elusive. To examine the response to wounding in Physcomitrella patens, a model moss, a proteomic analysis of wounded P. patens was conducted. Proteomic analysis showed that wounding increased the abundance of proteins related to protein synthesis, amino acid metabolism, protein folding, photosystem, glycolysis, and energy synthesis. 12-Oxo-phytodienoic acid (OPDA) was induced by wounding and inhibited growth. Therefore, OPDA is considered a signaling molecule in this plant. Proteomic analysis of a P. patens mutant in which the PpAOS1 and PpAOS2 genes, which are involved in OPDA biosynthesis, are disrupted showed accumulation of proteins involved in protein synthesis in response to wounding in a similar way to the wild-type plant. In contrast, the fold-changes of the proteins in the wild-type plant were significantly different from those in the aos mutant. This study suggests that PpAOS gene expression enhances photosynthesis and effective energy utilization in response to wounding in P. patens.

scholarly journals Proteomic Analysis of Morphologically Changed Tissues after Prolonged Dexamethasone Treatment

2019 ◽  
Vol 20 (13) ◽  
pp. 3122 ◽  
Author(s):  
Abeer K. Malkawi ◽  
Afshan Masood ◽  
Zakia Shinwari ◽  
Minnie Jacob ◽  
Hicham Benabdelkamel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Proteomic Analysis ◽  
Acid Metabolism ◽  
Muscular Atrophy ◽  
Liver Lipid ◽  
Nervous System Development ◽  
Nucleic Acid Metabolism ◽  
Biochemical Pathways ◽  
Proteomic Approach ◽  
And Function

Prolonged dexamethasone (Dex) administration leads to serious adverse and decrease brain and heart size, muscular atrophy, hemorrhagic liver, and presence of kidney cysts. Herein, we used an untargeted proteomic approach using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for simultaneous identification of changes in proteomes of the major organs in Sprague–Dawley (SD rats post Dex treatment. The comparative and quantitative proteomic analysis of the brain, heart, muscle, liver, and kidney tissues revealed differential expression of proteins (n = 190, 193, 39, 230, and 53, respectively) between Dex-treated and control rats. Functional network analysis using ingenuity pathway analysis (IPA revealed significant differences in regulation of metabolic pathways within the morphologically changed organs that related to: (i) brain—cell morphology, nervous system development, and function and neurological disease; (ii) heart—cellular development, cellular function and maintenance, connective tissue development and function; (iii) skeletal muscle—nucleic acid metabolism, and small molecule biochemical pathways; (iv) liver—lipid metabolism, small molecular biochemistry, and nucleic acid metabolism; and (v) kidney—drug metabolism, organism injury and abnormalities, and renal damage. Our study provides a comprehensive description of the organ-specific proteomic profilesand differentially altered biochemical pathways, after prolonged Dex treatement to understand the molecular basis for development of side effects.

Proteomic Analysis of Dormant Seeds with Dry Ripening Process in Maize Inbred Lines

2014 ◽  
Vol 40 (10) ◽  
pp. 1865
Author(s):  
Hai LAN ◽  
Yi-Feng LENG ◽  
Shu-Feng ZHOU ◽  
Jian LIU ◽  
Ting-Zhao RONG
Keyword(s):  
Proteomic Analysis ◽  
Inbred Lines ◽  
Ripening Process ◽  
Maize Inbred Lines

scholarly journals Evaluation of Cell Wall Modification in Two Strawberry Cultivars with Contrasted Softness

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1100
Author(s):  
Ricardo I. Castro ◽  
Marcelo Muñoz-Vera ◽  
Luis Morales-Quintana
Keyword(s):  
Thermal Stability ◽  
Cell Wall ◽  
Cell Wall Modification ◽  
Ripening Process ◽  
Morphological Studies ◽  
Ripe Stage ◽  
New Evidence ◽  
Region Ii ◽  
Green Stage ◽  
Strawberry Cultivars

During the ripening process of fruit, the solubilization and depolymerization of cell wall components takes place, which results in the loss of firmness or the softening of fruit. Recently, we reported that two different strawberry cultivars (“Cristal” and “Portola”) exhibit differences in their fruit softening values, with “Cristal” being the firmest and “Portola” being the softest. In the present work, we performed a comparative study of the changes in the physicochemical properties of the cell wall-associated polysaccharide contents of these two strawberry fruit cultivars via thermogravimetric analysis (TGA), combined with the first derivative of the thermogram (DTG) curves and morphological studies using scanning electron microscopy (SEM). The “Cristal” sample showed higher thermal stability than the “Portola” sample. Additionally, differences were observed between the “Cristal” and “Portola” samples at different stages, principally in Region II (temperatures between 200 °C and 350 °C), with a higher thermal stability evident in the green stage of the two cultivars. Notably, a higher thermal stability was observed in the green stage of the “Portola” sample. The highest percentage of cumulative depolymerization (PCD) was observed in the ripe stage of the “Portola” sample. The DTG curve showed four maximum peaks of degradation, which occurred between 170 °C and 350 °C. Finally, the existence of a relationship between fruit firmness and thermal stability was demonstrated for the two cultivars. This relationship was based on the morphological studies conducted using SEM, which provided new evidence through which to understand the changes within the cell wall polymers of these two strawberry cultivars during the ripening process.

scholarly journals A DIGE-based quantitative proteomic analysis of grape berry flesh development and ripening reveals key events in sugar and organic acid metabolism

2011 ◽  
Vol 62 (8) ◽  
pp. 2521-2569 ◽  
Author(s):  
Maria José Martínez-Esteso ◽  
Susana Sellés-Marchart ◽  
Diego Lijavetzky ◽  
Maria Angeles Pedreño ◽  
Roque Bru-Martínez
Keyword(s):  
Organic Acid ◽  
Proteomic Analysis ◽  
Acid Metabolism ◽  
Grape Berry ◽  
Quantitative Proteomic Analysis ◽  
Organic Acid Metabolism ◽  
Key Events

scholarly journals Comparative Proteomics Reveals the Spoilage-Related Factors of Shewanella putrefaciens Under Refrigerated Condition

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhengkai Yi ◽  
Jing Xie
Keyword(s):  
Amino Acid ◽  
Proteomic Analysis ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Sulfur Metabolism ◽  
Shewanella Putrefaciens ◽  
Extracellular Proteins ◽  
Differentially Expressed ◽  
Differentially Expressed Protein ◽  
Fish Proteins

Shewanella putrefaciens is a microorganism with strong spoilage potential for aquatic products. This study aimed to investigate the potential spoilage factors of S. putrefaciens by comparative proteomic analysis. The spoilage potential of two strains of S. putrefaciens (00A and 00B) isolated from chilled spoiled bigeye tuna was investigated. The results of total volatile basic nitrogen (TVB-N), trimethylamine (TMA) in fish inoculated with S. putrefaciens, extracellular protease activity of S. putrefaciens, and degradation of fish proteins indicated that the spoilage potential of S. putrefaciens 00A was much higher than that of 00B. Fish proteins are usually degraded by spoilage microorganism proteases into small molecular peptides and amino acids, which are subsequently degraded into spoilage metabolites in bacterial cells, leading to deterioration of fish quality. Thus, proteomic analysis of the extracellular and intracellular proteins of 00A vs. 00B was performed. The results indicated that the intracellular differentially expressed protein (IDEP) contained 243 upregulated proteins and 308 downregulated proteins, while 78 upregulated proteins and 4 downregulated proteins were found in the extracellular differentially expressed protein (EDEP). GO annotation revealed that IDEP and EDEP were mainly involved in cellular and metabolic processes. KEGG annotation results showed that the upregulated proteins in IDEP were mainly involved in sulfur metabolism, amino acid metabolism, and aminoacyl-tRNA biosynthesis, while downregulated proteins were related to propanoate metabolism. In contrast, EDEP of KEGG annotation was mainly involved in ribosomes, quorum sensing, and carbohydrate metabolism. Proteins associated with spoilage containing sulfur metabolism (sulfite reductase, sulfate adenylyltransferase, adenylyl-sulfate kinase), amino acid metabolism (biosynthetic arginine decarboxylase, histidine ammonia-lyase), trimethylamine metabolism (trimethylamine-N-oxide reductase), and extracellular proteins (ATP-dependent Clp protease proteolytic subunit) were identified as upregulated. These proteins may play a key role in the spoilage potential of S. putrefaciens. These findings would contribute to the identification of key spoilage factors and understanding of the spoilage mechanism of microorganisms.

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