Distinct metabolic changes between wheat embryo and endosperm during grain development revealed by 2D-DIGE-based integrative proteome analysis

PROTEOMICS ◽  
2016 ◽  
Vol 16 (10) ◽  
pp. 1515-1536 ◽  
Author(s):  
Hui Cao ◽  
Miao He ◽  
Chong Zhu ◽  
Linlin Yuan ◽  
Liwei Dong ◽  
...  
Keyword(s):  
Proteome Analysis ◽  
Metabolic Changes ◽  
Grain Development ◽  
Wheat Embryo ◽  
2D Dige

Comparative proteome analysis of Actinoplanes utahensis grown on various saccharides based on 2D-DIGE and MALDI-TOF/TOF-MS

2021 ◽  
pp. 104193
Author(s):  
Chun-Yue Weng ◽  
Chao-Er Wang ◽  
Wei-Bang Xie ◽  
Shen-Yuan Xu ◽  
Ya-Jun Wang ◽  
...  
Keyword(s):  
Proteome Analysis ◽  
2D Dige ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Effects of Independent and Combined Water-Deficit and High-Nitrogen Treatments on Flag Leaf Proteomes during Wheat Grain Development

2020 ◽  
Vol 21 (6) ◽  
pp. 2098 ◽  
Author(s):  
Dong Zhu ◽  
Gengrui Zhu ◽  
Zhen Zhang ◽  
Zhimin Wang ◽  
Xing Yan ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Water Deficit ◽  
Flag Leaf ◽  
Redox Homeostasis ◽  
Combined Treatment ◽  
Proteome Analysis ◽  
N Fertilization ◽  
Grain Development ◽  
High Nitrogen ◽  
Nitrogen Treatments

We present the first comprehensive proteome analysis of wheat flag leaves under water-deficit, high-nitrogen (N) fertilization, and combined treatments during grain development in the field. Physiological and agronomic trait analyses showed that leaf relative water content, total chlorophyll content, photosynthetic efficiency, and grain weight and yield were significantly reduced under water-deficit conditions, but dramatically enhanced under high-N fertilization and moderately promoted under the combined treatment. Two-dimensional electrophoresis detected 72 differentially accumulated protein (DAP) spots representing 65 unique proteins, primarily involved in photosynthesis, signal transduction, carbohydrate metabolism, redox homeostasis, stress defense, and energy metabolism. DAPs associated with photosynthesis and protein folding showed significant downregulation and upregulation in response to water-deficit and high-N treatments, respectively. The combined treatment caused a moderate upregulation of DAPs related to photosynthesis and energy and carbohydrate metabolism, suggesting that high-N fertilization can alleviate losses in yield caused by water-deficit conditions by enhancing leaf photosynthesis and grain storage compound synthesis.

2D-DIGE A Powerful Tool for Proteome Analysis

2020 ◽  
pp. 67-73
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Great Sensitivity ◽  
Two Dimensional Gel Electrophoresis ◽  
2D Dige ◽  
Protein Sample ◽  
Different Types ◽  
Complex Protein ◽  
2D Gel

In the recent past, two dimensional gel electrophoresis has emerged as a powerful molecular biology tool for the comparative expression profiling of complex protein sample. It involves the separation as well as the resolution of diverse proteins sample on the basis of isoelectric points and molecular mass of protein in two dimension ways. In this way, it reflects the view of overall proteome status including differentiation in protein expression levels, post-translational modifications etc. Moreover, this allows the identification of novel biological signatures, which may give a particular identity of pathological background to cells or tissues associated with various types of cancers and neurological disorders. Therefore, by utilizing such tools, one can clearly investigate and compare the effects of particular drugs on cells of tissues and also one can analyze the effects of disease on the basis of variations in protein expression profile at broad spectrum. Recently, to get more error-less and accurate proteome profile, conventional 2-D gel electrophoresis has been enhanced with the inclusion of different types of protein labeling dyes which enables a more comparative analysis of diverse protein sample in a single 2-D gel. In this advanced technique (2-D-DIGE), protein samples are labeled with three different types of CyDyes (Cy2, Cy3, and Cy5) separately and combined and further resolved on the same gel. This will facilitate the more accurate spot matching on a single gel platform and will also minimize the experimental variations as commonly reported in the conventional 2D-gel electrophoresis. Therefore, in the present proteomic research era, 2D-DIGE has proved to be an extremely powerful tool with great sensitivity for up to 125 ng of proteins in clinical research volubility especially, neurological and cancer related disorders.

Differential Proteome Analysis Using 2D-DIGE

2021 ◽  
pp. 77-84
Author(s):  
Caroline May ◽  
Frederic Brosseron ◽  
Piotr Chartowski ◽  
Kristin Fuchs ◽  
Helmut E. Meyer ◽  
...  
Keyword(s):  
Proteome Analysis ◽  
2D Dige

Abstract 3734: Differential Platelet Proteome Analysis Following Specific Activation Of Glycoprotein VI

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Christian Schulz ◽  
Nina V Leuschen ◽  
Thomas Fröhlich ◽  
Michael Lorenz ◽  
Georg J Arnold ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Membrane Trafficking ◽  
Proteome Analysis ◽  
Multivesicular Body ◽  
Healthy Human ◽  
Body Protein ◽  
2D Dige ◽  
Glycoprotein Vi ◽  
Platelet Proteome ◽  
Differentially Abundant Proteins

Background: Platelets play a key role in haemostasis and in the pathophysiology of various diseases including arterial thrombosis. Glycoprotein (GP)VI, the major platelet collagen receptor, mediates platelet activation and firm adhesion to collagen structures exposed at sites of vascular injury. Here, we determined the effects of specific activation of GPVI on the human platelet proteome by two-dimensional differential in-gel electrophoresis (2D-DIGE) and mass spectrometry. Methods and Results: Platelets from healthy human donors were isolated and purified. Platelets were stimulated with an activating monoclonal antibody specific for GPVI, or control IgG. Platelet proteins were subjected to 2D-DIGE with extensive software-assisted image analysis. Subsequent identification of the differentially abundant proteins was carried out by MALDI TOF/TOF MS and LC ESI-MS/MS. We identified 8 differentially regulated proteins associated with cell signaling, metabolism, organization and rearrangement of the cytoskeleton, and membrane trafficking. Differentially abundant proteins included pleckstrin, beta-centractin, src substrate cortactin, charged multivesicular body protein 3, aldose reductase and protein disulfide isomerase-associated 3 precursor. Conclusion: Specific platelet activation via GPVI results in the differential regulation of several proteins. The results provide further insight into the mechanisms that underlie platelet activation through the GPVI receptor, which may help to identify novel pharmacological targets.

Upregulation of annexin A2 in H2O2-induced premature senescence as evidenced by 2D-DIGE proteome analysis

2008 ◽  
Vol 43 (4) ◽  
pp. 353-359 ◽  
Author(s):  
Aline Chrétien ◽  
Edouard Delaive ◽  
Marc Dieu ◽  
Catherine Demazy ◽  
Noëlle Ninane ◽  
...  
Keyword(s):  
Proteome Analysis ◽  
Annexin A2 ◽  
Premature Senescence ◽  
2D Dige
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