scholarly journals Energetics and structural characterization of isomers using ion mobility and gas-phase H/D exchange: Learning from lasso peptides

PROTEOMICS ◽  
2015 ◽  
Vol 15 (16) ◽  
pp. 2823-2834 ◽  
Author(s):  
Emeline Hanozin ◽  
Denis Morsa ◽  
Edwin De Pauw
Keyword(s):  
Gas Phase ◽  
Structural Characterization ◽  
Ion Mobility ◽  
Lasso Peptides

scholarly journals State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis

2021 ◽  
Vol 14 (6) ◽  
pp. 498
Author(s):  
Evolène Deslignière ◽  
Anthony Ehkirch ◽  
Bastiaan L. Duivelshof ◽  
Hanna Toftevall ◽  
Jonathan Sjögren ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility ◽  
State Of The Art ◽  
Native Mass Spectrometry ◽  
Site Specific ◽  
Drug Conjugates ◽  
Conjugation Process ◽  
Antibody Drug

Antibody-drug conjugates (ADCs) are biotherapeutics consisting of a tumor-targeting monoclonal antibody (mAb) linked covalently to a cytotoxic drug. Early generation ADCs were predominantly obtained through non-selective conjugation methods based on lysine and cysteine residues, resulting in heterogeneous populations with varying drug-to-antibody ratios (DAR). Site-specific conjugation is one of the current challenges in ADC development, allowing for controlled conjugation and production of homogeneous ADCs. We report here the characterization of a site-specific DAR2 ADC generated with the GlyCLICK three-step process, which involves glycan-based enzymatic remodeling and click chemistry, using state-of-the-art native mass spectrometry (nMS) methods. The conjugation process was monitored with size exclusion chromatography coupled to nMS (SEC-nMS), which offered a straightforward identification and quantification of all reaction products, providing a direct snapshot of the ADC homogeneity. Benefits of SEC-nMS were further demonstrated for forced degradation studies, for which fragments generated upon thermal stress were clearly identified, with no deconjugation of the drug linker observed for the T-GlyGLICK-DM1 ADC. Lastly, innovative ion mobility-based collision-induced unfolding (CIU) approaches were used to assess the gas-phase behavior of compounds along the conjugation process, highlighting an increased resistance of the mAb against gas-phase unfolding upon drug conjugation. Altogether, these state-of-the-art nMS methods represent innovative approaches to investigate drug loading and distribution of last generation ADCs, their evolution during the bioconjugation process and their impact on gas-phase stabilities. We envision nMS and CIU methods to improve the conformational characterization of next generation-empowered mAb-derived products such as engineered nanobodies, bispecific ADCs or immunocytokines.

scholarly journals Structural Characterization of Methylenedianiline Regioisomers by Ion Mobility-Mass Spectrometry and Tandem Mass Spectrometry. 4. 3-Ring and 4-Ring Isomers

2018 ◽  
Vol 90 (24) ◽  
pp. 14453-14461 ◽  
Author(s):  
Tiffany M. Crescentini ◽  
Sarah M. Stow ◽  
Jay G. Forsythe ◽  
Jody C. May ◽  
John A. McLean ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Structural Characterization ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Tandem Mass

scholarly journals Structural characterization of holo- and apo-myoglobin in the gas phase by ultraviolet photodissociation mass spectrometry

2015 ◽  
Vol 6 (2) ◽  
pp. 1324-1333 ◽  
Author(s):  
Michael B. Cammarata ◽  
Jennifer S. Brodbelt
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Structural Characterization ◽  
Ultraviolet Photodissociation ◽  
193 Nm

193 nm UV photodissociation of myoglobin in the gas phase showed preferential backbone cleavages in regions with higher relative B-factors.

scholarly journals Structural characterization of nucleotide 5′-triphosphates by infrared ion spectroscopy and theoretical studies

2018 ◽  
Vol 20 (44) ◽  
pp. 28319-28330 ◽  
Author(s):  
Rianne E. van Outersterp ◽  
Jonathan Martens ◽  
Giel Berden ◽  
Jeffrey D. Steill ◽  
Jos Oomens ◽  
...  
Keyword(s):  
Gas Phase ◽  
Structural Properties ◽  
Structural Characterization ◽  
Spectroscopic Studies ◽  
Theoretical Studies ◽  
Ion Spectroscopy ◽  
Ir Spectroscopic

Gas-phase IR spectroscopic studies and supporting theoretical studies have been performed on adenosine 5′-triphosphate, cytosine 5′-triphosphate and guanosine 5′-triphosphate to elucidate the structural properties of the NTPs by focusing on the influence of the nucleobase and the extent of deprotonation.

Structural Characterization of 3C-SiC Grown Using Methyltrichlorosilane

2013 ◽  
Vol 740-742 ◽  
pp. 291-294 ◽  
Author(s):  
Matteo Bosi ◽  
Giovanni Attolini ◽  
Béla Pécz ◽  
Zsolt Zolnai ◽  
László Dobos ◽  
...  
Keyword(s):  
Residual Stress ◽  
Gas Phase ◽  
Structural Characterization ◽  
Amorphous Content ◽  
Si Substrates

3C-SiC layers were grown on Si substrates using standard precursors (SiH4and C3H8) and by adding methyl trichloro silane (MTS) to the gas phase, with growth temperatures between 1200 and 1300 °C. Characterization of the 3C-SiC layers shows that 3C-SiC grown with MTS has higher polycrystalline and amorphous content as well as lower residual stress.

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