Abundant storage protein depletion from tuber proteins using ethanol precipitation method: Suitability to proteomics study

PROTEOMICS ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 1765-1769 ◽  
Author(s):  
Hye Min Lee ◽  
Ravi Gupta ◽  
Sun Hyung Kim ◽  
Yiming Wang ◽  
Randeep Rakwal ◽  
...  
Keyword(s):  
Storage Protein ◽  
Precipitation Method ◽  
Protein Depletion ◽  
Ethanol Precipitation ◽  
Proteomics Study ◽  
Method Suitability

scholarly journals A Novel Inulin-Mediated Ethanol Precipitation Method for Separating Endo-Inulinase From Inulinases for Inulooligosaccharides Production From Inulin

2021 ◽  
Vol 9 ◽  
Author(s):  
Xin Li ◽  
Qiannan Zhang ◽  
Wei Wang ◽  
Shang-Tian Yang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Functional Food ◽  
Degree Of Polymerization ◽  
Precipitation Method ◽  
High Yield ◽  
Process Conditions ◽  
Food Ingredients ◽  
Ethanol Precipitation ◽  
Novel Method

Inulin is a kind of polysaccharide that can be obtained various biomass. Inulooligosaccharides (IOS), a kind of oligosaccharides that can be obtained from inulin by enzymatic hydrolysis using inulinases, have been regarded as the functional food ingredients. Commercially available inulinases produced by natural Aspergillus niger contained both endo- and exo-inulinase activities. For IOS production from inulin, it is desirable to use only endo-inulinase as exo-inulinase would produce mainly the monosacchairde fructose from inulin. In the present study, a simple inulin-mediated ethanol precipitation method was developed to separate endo- and exo-inulinases present in natural inulinases. IOS production from inulin using the enriched endo-inulinase was then optimized in process conditions including pH and temperature, achieving a high yield of ∼94%. The resultant IOS products had a degree of polymerization ranging from 2 to 7. The study demonstrated a novel method for obtaining partially purified or enriched endo-inulinase for IOS production from inulin in an efficient process.

scholarly journals Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

2016 ◽  
Vol 11 (3) ◽  
pp. 573 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Rajib Majumder ◽  
Irfan A. Rather ◽  
Kangmin Kim
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Trichloroacetic Acid ◽  
Freeze Drying ◽  
Precipitation Method ◽  
Isolation And Purification ◽  
Video Clips ◽  
Response System ◽  
Ethanol Precipitation ◽  
Scanning Electron

<p>Lactic acid bacteria are classified ‘Generally Recognized As Safe’ (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.</p><p><strong>Video Clips</strong></p><p><a href="https://www.youtube.com/v/PGDyHPEk5jY">Part 1</a>:   7 min 42 sec</p><p><a href="https://youtube.com/v/G8ZMZ4z1DMs">Part 2</a>:   3 min 36 sec</p>

Limit Test of Aristolochic Acid a in YishenJuanbi Pill

2013 ◽  
Vol 634-638 ◽  
pp. 991-995
Author(s):  
Xing Cui Qu ◽  
Li Ma ◽  
Feng Han ◽  
Jing Gao
Keyword(s):  
Detection Limit ◽  
Aristolochic Acid ◽  
High Specificity ◽  
Hplc Method ◽  
Water Extraction ◽  
Precipitation Method ◽  
Ethanol Precipitation ◽  
Specificity And Sensitivity ◽  
Limit Test ◽  
Hplc Technique

We developed a HPLC method with high specificity and sensitivity for the limit test of AAA in Yishen Juanbi Pill. In this mothod, the detection limit for AAA was 2.216ng. AAA could not be detected in all the 3 batches of the pill which suggests that the pill prepared by the modified water extraction and ethanol precipitation method is safe for clinical use.This efficient HPLC technique is also proposed for the limit test of AAA in other preparations besides Yishen Juanbi Pill.

scholarly journals Purification of Tea saponins and Evaluation of its Effect on Alcohol Dehydrogenase Activity

2018 ◽  
Vol 13 (1) ◽  
pp. 56-63 ◽  
Author(s):  
Chuanxun Yuan ◽  
Yan Li ◽  
Qingchuan Li ◽  
Risheng Jin ◽  
Lili Ren
Keyword(s):  
Alcohol Dehydrogenase ◽  
Precipitation Method ◽  
Optimum Conditions ◽  
Liquid Ratio ◽  
Potent Effect ◽  
Ethanol Precipitation ◽  
Alcohol Dehydrogenase Activity ◽  
Ftir Spectrometry ◽  
Solid Liquid ◽  
Extraction Agent

AbstractTea saponins, extracted from a Camellia oleifera cake, were found to have a potent effect on de-alcoholic activity. To obtain highly pure tea saponins, which can better maintain the activity of alcohol dehydrogenase (ADH), this paper presents an extraction method for tea saponins using deionized water as the extraction agent and a two-stage precipitation method, including ethanol precipitation and CaO precipitation. The optimum conditions for ethanol precipitation were 95% alcohol, a duration of 1.5h and a solid/liquid ratio of 1:4; while the optimum conditions for CaO precipitation were a duration of 2h and an NH4HCO3/CaO ratio of 2:1. Under the optimum conditions, the content of saponins was 87.58%. The results showed that the greater the amount of tea saponins and the higher its purity, the more significant its activating effect on ADH. When the purity of tea saponins was above 75%, it activated ADH. It indicated that the de-alcoholic mechanism of tea saponins is associated with the activity of ADH. Furthermore, the study characterized the structure of tea saponins by UV absorption and Fourier Transform Infrared (FTIR) spectrometry and LC-MS.

Where is the prolamine located in rice endosperm?

1990 ◽  
Vol 48 (3) ◽  
pp. 696-697
Author(s):  
Hsin-Kan Wu ◽  
Mei-Chu Chung
Keyword(s):  
Storage Protein ◽  
Sodium Phosphate ◽  
Recent Experiment ◽  
Protein A ◽  
Uranyl Acetate ◽  
Protein Bodies ◽  
Lead Citrate ◽  
Thin Sections ◽  
Rice Endosperm ◽  
Ethanol Series

In one of our earlier papers (Wu et al. 1978), we suggested that glutelin is the major composition of the round storage protein bodies although they also contain relatively more prolamine than the angular one does. Immunochemical studies of Krishnan et al. (1986) later showed the presence of glutelin in the irregularly-shaped (angular) protein bodies while the prolamines were found in the round ones. Our recent experiment using protein A-gold technique found that prolamine is mainly deposited into the angular protein bodies.Small blocks (1 mm3) of 7 DAF (days after flowering) caryopsis of Orvza perennis were fixed with 3% paraformaldehyde and 3% glutaraldehyde in 0.1M sodium phosphate, pH7.4, dehydrated in a graded ethanol series and infiltrated with Spurr’s resin. Thin sections, after gold labeling, were stained with uranyl acetate and lead citrate. Rabbit antibodies were raised against purified prolamine. Protein A-gold sol complex was prepared based on the technique of Horisberger et al. (1977).

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