On the mechanism of the cold ethanol precipitation method of plasma protein fractionation

1989 ◽  
Vol 8 (5) ◽  
pp. 661-668 ◽  
Author(s):  
C. J. van Oss
Keyword(s):  
Plasma Protein ◽  
Precipitation Method ◽  
Protein Fractionation ◽  
Ethanol Precipitation

scholarly journals A Novel Inulin-Mediated Ethanol Precipitation Method for Separating Endo-Inulinase From Inulinases for Inulooligosaccharides Production From Inulin

2021 ◽  
Vol 9 ◽  
Author(s):  
Xin Li ◽  
Qiannan Zhang ◽  
Wei Wang ◽  
Shang-Tian Yang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Functional Food ◽  
Degree Of Polymerization ◽  
Precipitation Method ◽  
High Yield ◽  
Process Conditions ◽  
Food Ingredients ◽  
Ethanol Precipitation ◽  
Novel Method

Inulin is a kind of polysaccharide that can be obtained various biomass. Inulooligosaccharides (IOS), a kind of oligosaccharides that can be obtained from inulin by enzymatic hydrolysis using inulinases, have been regarded as the functional food ingredients. Commercially available inulinases produced by natural Aspergillus niger contained both endo- and exo-inulinase activities. For IOS production from inulin, it is desirable to use only endo-inulinase as exo-inulinase would produce mainly the monosacchairde fructose from inulin. In the present study, a simple inulin-mediated ethanol precipitation method was developed to separate endo- and exo-inulinases present in natural inulinases. IOS production from inulin using the enriched endo-inulinase was then optimized in process conditions including pH and temperature, achieving a high yield of ∼94%. The resultant IOS products had a degree of polymerization ranging from 2 to 7. The study demonstrated a novel method for obtaining partially purified or enriched endo-inulinase for IOS production from inulin in an efficient process.

High-resolution plasma protein fractionation using ultrafiltration

Desalination ◽  
2002 ◽  
Vol 144 (1-3) ◽  
pp. 301-306 ◽  
Author(s):  
Yinhua Wan ◽  
Raja Ghosh ◽  
Zhanfeng Cui
Keyword(s):  
High Resolution ◽  
Plasma Protein ◽  
Protein Fractionation

scholarly journals A plasma protein fractionation procedure for use in studies of protein metabolism. Its application to the measurement of synthesis rates of two α1-glycoproteins and other serum proteins in the rat

1974 ◽  
Vol 141 (3) ◽  
pp. 655-665 ◽  
Author(s):  
J. Ho ◽  
K. N. Jeejeebhoy ◽  
R. H. Painter
Keyword(s):  
Electrophoretic Mobility ◽  
Plasma Protein ◽  
Protein Metabolism ◽  
Plasma Proteins ◽  
Serum Proteins ◽  
Single Sample ◽  
Protein Fractionation ◽  
Step Method ◽  
Preparative Scale ◽  
Fractionation Procedure

A two-step method for the separation of five different plasma proteins on a preparative scale, which is capable of being extended to allow the separation of other plasma proteins, is described. The proteins separated were fibrinogen, two α1-glycoproteins, albumin and transferrin. The α1-glycoproteins were characterized in terms of electrophoretic mobility, ultracentrifugal and immunological characteristics. By using this method, it was shown that a single sample of plasma could be fractionated to yield purified proteins in sufficient quantity to simultaneously measure the synthesis of the two α1-glycoproteins, albumin and transferrin in the rat with McFarlane's technique (McFarlane, 1963; Reeve et al., 1963; McFarlane et al., 1965).

Plasma Protein Fractionation

1977 ◽  
pp. 545-597 ◽  
Author(s):  
K. Heide ◽  
H. Haupt ◽  
H.G. Schwick
Keyword(s):  
Plasma Protein ◽  
Protein Fractionation

Preparative plasma protein fractionation by isotachophoresis in sephadex columns

1977 ◽  
Vol 132 (3) ◽  
pp. 437-450 ◽  
Author(s):  
M. Bier ◽  
R.M. Cuddeback ◽  
A. Kopwillem
Keyword(s):  
Plasma Protein ◽  
Protein Fractionation
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