scholarly journals Label-free detection techniques for protein microarrays: Prospects, merits and challenges

PROTEOMICS ◽  
2009 ◽  
Vol 10 (4) ◽  
pp. 731-748 ◽  
Author(s):  
Sandipan Ray ◽  
Gunjan Mehta ◽  
Sanjeeva Srivastava
Keyword(s):  
Protein Microarrays ◽  
Label Free ◽  
Detection Techniques ◽  
Label Free Detection

scholarly journals Label and Label-Free Detection Techniques for Protein Microarrays

Microarrays ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 228-244 ◽  
Author(s):  
Amir Syahir ◽  
Kenji Usui ◽  
Kin-ya Tomizaki ◽  
Kotaro Kajikawa ◽  
Hisakazu Mihara
Keyword(s):  
Protein Microarrays ◽  
Label Free ◽  
Detection Techniques ◽  
Label Free Detection

Label-free detection of nucleic acid and protein microarrays by scanning Kelvin nanoprobe

2005 ◽  
Vol 20 (8) ◽  
pp. 1471-1481 ◽  
Author(s):  
Michael Thompson ◽  
Larisa-Emilia Cheran ◽  
Mingquan Zhang ◽  
Melissa Chacko ◽  
Hong Huo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Microarrays ◽  
Label Free ◽  
Label Free Detection

Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

2003 ◽  
Vol 773 ◽  
Author(s):  
James P. Landry ◽  
X. D. Zhu ◽  
X. W. Guo ◽  
J. P. Gregg
Keyword(s):  
Oblique Incidence ◽  
Protein Microarrays ◽  
Fluorescent Labeling ◽  
Fluorescent Label ◽  
Label Free ◽  
Detection Techniques ◽  
Glass Substrates ◽  
Standard Glass ◽  
Non Destructive ◽  
Microarray Detection

AbstractWe have constructed an oblique-incidence reflectivity difference (OI-RD) microscope for fluorescent label-free imaging of DNA and protein microarrays on standard glass substrates. Using both OI-RD and fluorescence images, we demonstrate a difference in wetting behavior of labeled and unlabeled IgG protein molecules deposited on an aldehyde-derivatized glass surface. The potential of fluorescent labeling agents to influence the properties of proteins highlights the need for label-free microarray detection techniques to supplement existing fluorescence methods. We also present OI-RD images of an oligonucleotide microarray after printing and washing procedures to demonstrate the use of OI-RD for non-destructive monitoring of changes in the optical properties of microarrays during processing.

scholarly journals Advances in Label-Free Detections for Nanofluidic Analytical Devices

Micromachines ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 885 ◽  
Author(s):  
Thu Le ◽  
Hisashi Shimizu ◽  
Kyojiro Morikawa
Keyword(s):  
Single Molecule ◽  
Detection Methods ◽  
Label Free ◽  
Practical Applications ◽  
Detection Techniques ◽  
Kinetic Information ◽  
Label Free Detection ◽  
Nanofluidic Devices ◽  
Nanofluidic Device ◽  
New Research

Nanofluidics, a discipline of science and engineering of fluids confined to structures at the 1–1000 nm scale, has experienced significant growth over the past decade. Nanofluidics have offered fascinating platforms for chemical and biological analyses by exploiting the unique characteristics of liquids and molecules confined in nanospaces; however, the difficulty to detect molecules in extremely small spaces hampers the practical applications of nanofluidic devices. Laser-induced fluorescence microscopy with single-molecule sensitivity has been so far a major detection method in nanofluidics, but issues arising from labeling and photobleaching limit its application. Recently, numerous label-free detection methods have been developed to identify and determine the number of molecules, as well as provide chemical, conformational, and kinetic information of molecules. This review focuses on label-free detection techniques designed for nanofluidics; these techniques are divided into two groups: optical and electrical/electrochemical detection methods. In this review, we discuss on the developed nanofluidic device architectures, elucidate the mechanisms by which the utilization of nanofluidics in manipulating molecules and controlling light–matter interactions enhances the capabilities of biological and chemical analyses, and highlight new research directions in the field of detections in nanofluidics.

Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method

2012 ◽  
Vol 55 (12) ◽  
pp. 2347-2350 ◽  
Author(s):  
Jun Dai ◽  
Lin Li ◽  
JingYi Wang ◽  
LiPing He ◽  
HuiBin Lu ◽  
...  
Keyword(s):  
Difference Method ◽  
Oblique Incidence ◽  
Protein Microarrays ◽  
Label Free ◽  
Label Free Detection

scholarly journals Emerging tools for real-time label-free detection of interactions on functional protein microarrays

FEBS Journal ◽  
2005 ◽  
Vol 272 (21) ◽  
pp. 5412-5425 ◽  
Author(s):  
Niroshan Ramachandran ◽  
Dale N. Larson ◽  
Peter R. H. Stark ◽  
Eugenie Hainsworth ◽  
Joshua LaBaer
Keyword(s):  
Real Time ◽  
Protein Microarrays ◽  
Label Free ◽  
Functional Protein ◽  
Label Free Detection

Readout of protein microarrays using intrinsic time resolved UV fluorescence for label-free detection

PROTEOMICS ◽  
2004 ◽  
Vol 4 (6) ◽  
pp. 1703-1711 ◽  
Author(s):  
Hans-Martin Striebel ◽  
Peter Schellenberg ◽  
Paulius Grigaravicius ◽  
Karl Otto Greulich
Keyword(s):  
Protein Microarrays ◽  
Label Free ◽  
Time Resolved ◽  
Uv Fluorescence ◽  
Intrinsic Time ◽  
Label Free Detection

Label-free detection methods for protein microarrays

PROTEOMICS ◽  
2006 ◽  
Vol 6 (20) ◽  
pp. 5493-5503 ◽  
Author(s):  
Xiaobo Yu ◽  
Danke Xu ◽  
Quan Cheng
Keyword(s):  
Protein Microarrays ◽  
Detection Methods ◽  
Label Free ◽  
Label Free Detection

Integrated microfluidic chip with nanobiosensor for rapid and label-free detection of a specific gene

2017 ◽  
Vol 9 (24) ◽  
pp. 3619-3625 ◽  
Author(s):  
Congxiao Zhang ◽  
Xuefei Lv ◽  
Saeed Yasmeen ◽  
Hong Qing ◽  
Yulin Deng
Keyword(s):  
Food Safety ◽  
Point Of Care ◽  
Low Cost ◽  
Specific Gene ◽  
Label Free ◽  
Biomolecular Detection ◽  
Detection Techniques ◽  
Label Free Detection ◽  
Rapid Tests ◽  
Point Of Care Tests

Biomolecular detection techniques are tending to develop in terms of miniaturization, automation, rapidity, sensitivity and low cost, and these techniques are urgently needed as “point of care tests” or “rapid tests” in clinical diagnosis, environmental monitoring and food safety.

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