Readout of protein microarrays using intrinsic time resolved UV fluorescence for label-free detection

PROTEOMICS ◽  
2004 ◽  
Vol 4 (6) ◽  
pp. 1703-1711 ◽  
Author(s):  
Hans-Martin Striebel ◽  
Peter Schellenberg ◽  
Paulius Grigaravicius ◽  
Karl Otto Greulich
Keyword(s):  
Protein Microarrays ◽  
Label Free ◽  
Time Resolved ◽  
Uv Fluorescence ◽  
Intrinsic Time ◽  
Label Free Detection

Label-free detection of nucleic acid and protein microarrays by scanning Kelvin nanoprobe

2005 ◽  
Vol 20 (8) ◽  
pp. 1471-1481 ◽  
Author(s):  
Michael Thompson ◽  
Larisa-Emilia Cheran ◽  
Mingquan Zhang ◽  
Melissa Chacko ◽  
Hong Huo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Microarrays ◽  
Label Free ◽  
Label Free Detection

scholarly journals Label-free detection techniques for protein microarrays: Prospects, merits and challenges

PROTEOMICS ◽  
2009 ◽  
Vol 10 (4) ◽  
pp. 731-748 ◽  
Author(s):  
Sandipan Ray ◽  
Gunjan Mehta ◽  
Sanjeeva Srivastava
Keyword(s):  
Protein Microarrays ◽  
Label Free ◽  
Detection Techniques ◽  
Label Free Detection

High-Throughput and Label-Free Detection of Motile Parasites in Bodily Fluids Using Lensless Time-Resolved Speckle Imaging

2019 ◽  
Author(s):  
Yibo Zhang ◽  
Hatice Ceylan Koydemir ◽  
Michelle M. Shimogawa ◽  
Sener Yalcin ◽  
Alexander Guziak ◽  
...  
Keyword(s):  
High Throughput ◽  
Label Free ◽  
Speckle Imaging ◽  
Time Resolved ◽  
Bodily Fluids ◽  
Label Free Detection

Label-Free Detection of Ovarian Cancer Antigen CA125 by Surface Enhanced Raman Scattering

2020 ◽  
Vol 20 (3) ◽  
pp. 1358-1365 ◽  
Author(s):  
İlknur TunÇ ◽  
Hepi Hari Susapto
Keyword(s):  
Raman Spectroscopy ◽  
Organic Molecules ◽  
Concentration Level ◽  
Hot Spot ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Time Resolved ◽  
Label Free Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) has drawn attention in recent years for imaging biologicalmolecules as an analytical tool due to its label-free approach. The SERS approach can be used in tracking organic molecules and monitoring unique Raman spectra of the organic molecules bound to metal nanoparticles (NPs). In this paper, the molecular specifity of Raman Spectroscopy was used together with self-assembled monolayer of metallic AuNPs as a sensor platform in order to detect CA125 antibody-antigen probe molecules. Highly enhanced electromagnetic fields localized around neighboring AuNPs provide hot-spot construction due to the spatial distribution of SERS enhancement on the CA125 proteins at nM concentration level. Time resolved SERS mapping of CA125 antibody and antigen couples was recorded. Even though blinking behavior was observed for some cases, vast variety SERS signals from CA125 proteins were highly reproducible. Blinking behavior is attributed to single molecular detection. Distinguished feature of SERS mapping images of CA125 antibody and antigen with such a low concentration level is very promising for this technique to be used for diagnostic purposes.

Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method

2012 ◽  
Vol 55 (12) ◽  
pp. 2347-2350 ◽  
Author(s):  
Jun Dai ◽  
Lin Li ◽  
JingYi Wang ◽  
LiPing He ◽  
HuiBin Lu ◽  
...  
Keyword(s):  
Difference Method ◽  
Oblique Incidence ◽  
Protein Microarrays ◽  
Label Free ◽  
Label Free Detection

scholarly journals Label and Label-Free Detection Techniques for Protein Microarrays

Microarrays ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 228-244 ◽  
Author(s):  
Amir Syahir ◽  
Kenji Usui ◽  
Kin-ya Tomizaki ◽  
Kotaro Kajikawa ◽  
Hisakazu Mihara
Keyword(s):  
Protein Microarrays ◽  
Label Free ◽  
Detection Techniques ◽  
Label Free Detection

scholarly journals Emerging tools for real-time label-free detection of interactions on functional protein microarrays

FEBS Journal ◽  
2005 ◽  
Vol 272 (21) ◽  
pp. 5412-5425 ◽  
Author(s):  
Niroshan Ramachandran ◽  
Dale N. Larson ◽  
Peter R. H. Stark ◽  
Eugenie Hainsworth ◽  
Joshua LaBaer
Keyword(s):  
Real Time ◽  
Protein Microarrays ◽  
Label Free ◽  
Functional Protein ◽  
Label Free Detection

Label-free detection methods for protein microarrays

PROTEOMICS ◽  
2006 ◽  
Vol 6 (20) ◽  
pp. 5493-5503 ◽  
Author(s):  
Xiaobo Yu ◽  
Danke Xu ◽  
Quan Cheng
Keyword(s):  
Protein Microarrays ◽  
Detection Methods ◽  
Label Free ◽  
Label Free Detection
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