Characterization of the human urine proteome by preparative electrophoresis in combination with 2-DE

PROTEOMICS ◽  
2006 ◽  
Vol 6 (15) ◽  
pp. 4346-4355 ◽  
Author(s):  
Panagiotis G. Zerefos ◽  
Konstantinos Vougas ◽  
Ploumisti Dimitraki ◽  
Sophia Kossida ◽  
Andreas Petrolekas ◽  
...  
Keyword(s):  
Human Urine ◽  
Preparative Electrophoresis ◽  
Urine Proteome

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

scholarly journals The Characterization of Human Urine for Specimen Validity Determination in Workplace Drug Testing: A Review

2000 ◽  
Vol 24 (7) ◽  
pp. 579-588 ◽  
Author(s):  
J. D. Cook ◽  
Y. H. Caplan ◽  
C. P. LoDico ◽  
D. M. Bush
Keyword(s):  
Human Urine ◽  
Drug Testing ◽  
Workplace Drug Testing

scholarly journals GREEN SYNTHESIS AND SPECTROSCOPIC CHARACTERIZATION OF IRON (III) COMPLEX OF UREA FROM HUMAN URINE AND IRON RUST

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
V.O. Uduah ◽  
J.J. Gongden ◽  
M.L. Kagoro ◽  
K.K. Gurumyen ◽  
Y.N. Lohdip ◽  
...  
Keyword(s):  
Human Urine ◽  
Synthesis Method ◽  
Reaction Medium ◽  
Spectroscopic Characterization ◽  
Waste Materials ◽  
Spectroscopic Techniques ◽  
Distilled Water ◽  
Oxide Composition ◽  
Ft Ir

This work presents a dry synthesis of Iron (III) complex with urea isolated from human urine and Fe (III) obtained from iron rust particles. Iron (III), PI (Purified iron rust), was isolated from iron rust in 10% hydrochloric acid, HCl and distilled water respectively. The complex was synthesized via dry-synthesis method using the melted urea as reaction medium. The isolated Fe (III) was characterized by elemental analysis which was done using XRF Cu-Zn method. The complex was prepared in a 1:4 metal to ligand (M-L) ratio. The stoichiometry of reaction indicate a 1:3 ratio of M-L (Fe-U). The complex was characterized by FT-IR, UV-vis, XRF and XRD spectroscopic techniques. The Fe (III) isolate and Fe-U complex shows percentage yields of 35.7% and ~92% respectively. The elemental and oxide composition of Fe and Fe2O3 (i.e., PI) were 40.387% and 57.753% respectively. The results obtained from the characterization of the iron-urea complex, IUC, indicate FT-IR result as symmetric and asymmetric frequencies with peaks of a combination band of Vs (NH) and Vas (NH), C=O and V (C-N) all stretched, XRD showed the crystal to be amorphous. The elemental and oxide composition of the Fe and Fe2O3 in IUC were 40.007 and 44.201 respectively. The results obtained revealed that useful complexes can be synthesized easily from waste materials, such as urine and iron rust particles, which complement Green chemistry.

scholarly journals Proof-of-Concept Workflow for Establishing Reference Intervals of Human Urine Proteome for Monitoring Physiological and Pathological Changes

EBioMedicine ◽  
2017 ◽  
Vol 18 ◽  
pp. 300-310 ◽  
Author(s):  
Wenchuan Leng ◽  
Xiaotian Ni ◽  
Changqing Sun ◽  
Tianyuan Lu ◽  
Anna Malovannaya ◽  
...  
Keyword(s):  
Human Urine ◽  
Reference Intervals ◽  
Proof Of Concept ◽  
Pathological Changes ◽  
Urine Proteome

Human Urine Proteome: A Powerful Source for Clinical Research

Urine ◽  
2019 ◽  
pp. 9-24
Author(s):  
Xiaolian Xiao ◽  
Lili Zou ◽  
Wei Sun
Keyword(s):  
Clinical Research ◽  
Human Urine ◽  
Powerful Source ◽  
Urine Proteome

scholarly journals Isolation and characterization of a precursor form of lysosomal alpha-glucosidase from human urine

1984 ◽  
Vol 139 (3) ◽  
pp. 489-495 ◽  
Author(s):  
Ronald P. J. OUDE ELFERINK ◽  
Elisabeth M. BROUWER-KELDER ◽  
Inge SURYA ◽  
Anneke STRIJLAND ◽  
Marian KROOS ◽  
...  
Keyword(s):  
Human Urine ◽  
Isolation And Characterization ◽  
Alpha Glucosidase

scholarly journals Detection and characterization of prednisolone metabolites in human urine by LC-MS/MS

2015 ◽  
Vol 50 (3) ◽  
pp. 633-642 ◽  
Author(s):  
Xavier Matabosch ◽  
Oscar J. Pozo ◽  
Clara Pérez-Mañá ◽  
Esther Papaseit ◽  
Jordi Segura ◽  
...  
Keyword(s):  
Human Urine
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