scholarly journals Isolation and characterization of N6-succinyladenosine from human urine

1977 ◽  
Vol 4 (3) ◽  
pp. 739-746 ◽  
Author(s):  
Girish B. Chheda
Keyword(s):  
Human Urine ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a precursor form of lysosomal alpha-glucosidase from human urine

1984 ◽  
Vol 139 (3) ◽  
pp. 489-495 ◽  
Author(s):  
Ronald P. J. OUDE ELFERINK ◽  
Elisabeth M. BROUWER-KELDER ◽  
Inge SURYA ◽  
Anneke STRIJLAND ◽  
Marian KROOS ◽  
...  
Keyword(s):  
Human Urine ◽  
Isolation And Characterization ◽  
Alpha Glucosidase

Isolation and Characterization of Glucuronic Acid Conjugates of Chlorpromazine in Human Urine

1959 ◽  
Vol 102 (3) ◽  
pp. 602-605 ◽  
Author(s):  
T. H. Lin ◽  
L. W. Reynolds ◽  
I. M. Rondish ◽  
E. J. Van Loon
Keyword(s):  
Human Urine ◽  
Glucuronic Acid ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a digoxin-like immunoreactive substance from human urine by affinity chromatography

1997 ◽  
Vol 43 (8) ◽  
pp. 1416-1420 ◽  
Author(s):  
Claudio De Angelis ◽  
Massimiliano Riscazzi ◽  
Riccardo Salvini ◽  
Alfonso Piccoli ◽  
Claudio Ferri ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Human Urine ◽  
Biological Fluids ◽  
Active Material ◽  
Reversed Phase ◽  
Cross Reactivity ◽  
Linear Gradient ◽  
Isolation And Characterization ◽  
Dog Kidney

Abstract A series of observations has suggested that one or more digoxin-like immunoreactive substances (DLIS) in biological fluids is able to cross-react with the antidigoxin antibody. Whether this substance is the endogenous inhibitor of Na+/K+ ATPase has not been well established. The aim of this study was to identify and characterize DLIS from human urine. Treated urine from healthy men was run on an affinity chromatography column at a flow rate of 1 mL/min in which the ligand was an antibody (antiserum) to digoxin. Eluates from affinity chromatography were applied onto analytical reversed-phase HPLC. The active material was eluted with a linear gradient of acetonitrile (from 350 to 650 mL/L) and water. A second step in HPLC was carried out isocratically with 280 mL/L acetonitrile in water. We found a single peak showing cross-reactivity with antidigoxin antibody as measured by RIA. It showed the same retention time as that of a digoxin calibrator. This highly purified substance is able to displace [3H]ouabain from dog kidney-derived Na+/K+ ATPase, to inhibit Na+/K+ ATPase activity as measured by the86Rb+ uptake in red blood cells and by coupled enzyme assay. Our results are consistent with the hypothesis that DLIS, as isolated by this particular digoxin antibody, is a single substance and an inhibitor of Na+/K+ ATPase.

Isolation and Characterization of 5-Carbamoylmethyluridine and 5-Carbamoylmethyl-2-Thiouridine from Human Urine

1999 ◽  
Vol 18 (10) ◽  
pp. 2155-2173 ◽  
Author(s):  
G. B. Chheda ◽  
H. B. Patrzyc ◽  
H. A. Tworek ◽  
S. P. Dutta
Keyword(s):  
Human Urine ◽  
Isolation And Characterization

Isolation and characterization of 1-β--ribofuranosylpyridin-4-one-3-carboxamide from human urine

Life Sciences ◽  
1979 ◽  
Vol 24 (15) ◽  
pp. 1381-1388 ◽  
Author(s):  
S.P. Dutta ◽  
P.F. Crain ◽  
J.A. McCloskey ◽  
G.B. Chheda
Keyword(s):  
Human Urine ◽  
Isolation And Characterization
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