Development and plasticity-related changes in protein expression patterns in cat visual cortex: A fluorescent two-dimensional difference gel electrophoresis approach

PROTEOMICS ◽  
2006 ◽  
Vol 6 (13) ◽  
pp. 3821-3832 ◽  
Author(s):  
Gert Van den Bergh ◽  
Stefan Clerens ◽  
Bonnie L. Firestein ◽  
Kalina Burnat ◽  
Lutgarde Arckens
2009 ◽  
Vol 7 (suppl_1) ◽  
Author(s):  
Laura E. McNamara ◽  
Matthew J. Dalby ◽  
Mathis O. Riehle ◽  
Richard Burchmore

Fluorescence two-dimensional difference gel electrophoresis (DiGE) is rapidly becoming established as a powerful technique for the characterization of differences in protein expression levels between two or more conditions. In this review, we consider the application of DiGE—both minimal and saturation labelling—to biomaterials research, considering the challenges and rewards of this approach.


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