Determining a significant change in protein expression with DeCyder™ during a pair-wise comparison using two-dimensional difference gel electrophoresis

PROTEOMICS ◽  
2004 ◽  
Vol 4 (5) ◽  
pp. 1421-1432 ◽  
Author(s):  
Natasha A. Karp ◽  
David P. Kreil ◽  
Kathryn S. Lilley
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Difference Gel Electrophoresis ◽  
Significant Change ◽  
Pair Wise Comparison

scholarly journals Fluorescence two-dimensional difference gel electrophoresis for biomaterial applications

2009 ◽  
Vol 7 (suppl_1) ◽  
Author(s):  
Laura E. McNamara ◽  
Matthew J. Dalby ◽  
Mathis O. Riehle ◽  
Richard Burchmore
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Expression Levels ◽  
Powerful Technique ◽  
Difference Gel Electrophoresis

Fluorescence two-dimensional difference gel electrophoresis (DiGE) is rapidly becoming established as a powerful technique for the characterization of differences in protein expression levels between two or more conditions. In this review, we consider the application of DiGE—both minimal and saturation labelling—to biomaterials research, considering the challenges and rewards of this approach.

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