A strategy for high-resolution protein identification in surface-enhanced laser desorption/ionization mass spectrometry: Calgranulin A and chaperonin 10 as protein markers for endometrial carcinoma

PROTEOMICS ◽  
2005 ◽  
Vol 5 (7) ◽  
pp. 1953-1966 ◽  
Author(s):  
Jingzhong Guo ◽  
Eric C. C. Yang ◽  
Leroi DeSouza ◽  
Georg Diehl ◽  
Mary Joe Rodrigues ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Identification ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Protein Markers ◽  
Desorption Ionization ◽  
Surface Enhanced ◽  
Chaperonin 10 ◽  
Calgranulin A

Identification of novel and downregulated biomarkers for alcoholism by surface enhanced laser desorption/ionization-mass spectrometry

PROTEOMICS ◽  
2004 ◽  
Vol 4 (4) ◽  
pp. 1187-1194 ◽  
Author(s):  
Fumio Nomura ◽  
Takeshi Tomonaga ◽  
Kazuyuki Sogawa ◽  
Tatsuya Ohashi ◽  
Masahiko Nezu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Surface Enhanced

Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry of Peritoneal Fluid Identifies Potential Biomarkers for Endometriosis

2009 ◽  
Vol 1 (3-4) ◽  
pp. 131-136 ◽  
Author(s):  
Monika M. Wölfler ◽  
Corinna Henkel ◽  
Daniela Hornung ◽  
Ruth Knüchel ◽  
Werner Rath ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Peritoneal Fluid ◽  
Protein Pattern ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Significant Information ◽  
Desorption Ionization ◽  
Protein Pattern Profiling ◽  
Surface Enhanced

Purpose Several markers for diagnosis of endometriosis that have been tested in clinical approaches so far lack in both sensitivity and specificity. Therefore, it is conceivable that a need exists to perform a cluster analysis in which the attributes of different markers are considered. Methods In this study, protein pattern profiling by surface-enhanced laser desorption/ionization time of flight (SELDI-TOF) mass spectrometry was performed to reveal differentially expressed pathognomonic proteins for endometriosis in peritoneal fluid samples of 106 symptomatic patients. Results At laparoscopy, 46 out of 106 (43.4%) patients exhibited endometriosis and 60 (56.6%) were proven disease-free. Using anionic affinity surfaces and a genetic algorithm for data analysis, 16 significantly different protein peaks were identified that allow for the prediction of endometriosis, with an overall recognition capability of 85.2%, exhibiting a sensitivity of 70.6% and a specificity of 80.8%. Conclusions Although lacking in sensitivity, this study provides highly significant information about differentially regulated proteins in endometriosis which might play a key role in the pathogenesis of this disease.

scholarly journals Influences of Blood Sample Processing on Low–Molecular-Weight Proteome Identified by Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry

2005 ◽  
Vol 51 (9) ◽  
pp. 1637-1649 ◽  
Author(s):  
Rosamonde E Banks ◽  
Anthea J Stanley ◽  
David A Cairns ◽  
Jennifer H Barrett ◽  
Paul Clarke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Plasma Samples ◽  
Laser Desorption Ionization ◽  
Serum Samples ◽  
Affinity Capture ◽  
Desorption Ionization ◽  
Elapsed Time ◽  
Surface Enhanced

Abstract Background: Profiling approaches in proteomics, such as surface-enhanced laser desorption/ionization (SELDI) mass spectrometry, are used in disease marker discovery. The aim of this study was to investigate the potential influence of selected preanalytical factors on the results obtained. Methods: Plasma samples anticoagulated with EDTA, citrate, or heparin, and serum samples from healthy volunteers were profiled by SELDI on CM10, immobilized metal affinity capture (IMAC) array with copper, and H50 chip surfaces. Using linear mixed-effects models, we examined the influence of elapsed time between venipuncture and sample separation (immediate to 24 h) and the type of serum tube used (Greiner Vacuette activator, gel serum separator, or plain tubes). We analyzed purified platelets, as well as platelet-poor and platelet-rich plasma samples treated with calcium and/or thrombin to determine the platelet contribution, directly or via the clotting process, to the profiles generated. We then used cluster analysis to identify samples with similar peak profiles. Results: Different plasma types and sera could be distinguished on the basis of cluster analyses of their spectral profiles. Elapsed time between venipuncture and separation of plasma and serum from blood samples altered the profiles obtained, particularly for serum samples and particularly on IMAC chips. The type of serum collection tube also affected the profiles because of differences in clotting time. In vitro manipulation of platelets revealed that specific peaks in IMAC profiles of serum appeared to be derived directly from platelets. Several other peaks, including some of those exhibiting time-dependent changes, arose during the clotting process. Conclusion: Preanalytical variables, such as sample handling, can markedly influence results.

A spherical covalent-organic framework for enhancing laser desorption/ionization mass spectrometry for small molecule detection

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3125-3130 ◽  
Author(s):  
Dan Ouyang ◽  
Kailong Luo ◽  
Wende Ma ◽  
Jie Wu ◽  
Jing Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Covalent Organic Framework ◽  
Desorption Ionization ◽  
Surface Enhanced ◽  
Small Molecule Detection ◽  
Organic Framework

A spherical vinyl-functionalized covalent-organic framework (COF-V) was prepared at room temperature by a facile method and applied as a novel substrate for surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS).

scholarly journals Magnetic nanoparticle modified chitosan for surface enhanced laser desorption/ionization mass spectrometry of surfactants

RSC Advances ◽  
2017 ◽  
Vol 7 (66) ◽  
pp. 41585-41592 ◽  
Author(s):  
Hani Nasser Abdelhamid ◽  
Yu Chih Lin ◽  
Hui-Fen Wu
Keyword(s):  
Mass Spectrometry ◽  
Magnetic Nanoparticle ◽  
Laser Desorption ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Modified Chitosan ◽  
Desorption Ionization ◽  
Surface Enhanced ◽  
Modified Magnetic Nanoparticles

Chitosan (CTS) modified magnetic nanoparticles (CTS@Fe3O4MNPs) offer dual functions for the detection of surfactants using surface enhanced laser desorption/ionization mass spectrometry (SELDI-MS).

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