Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry

PROTEOMICS ◽  
2004 ◽  
Vol 4 (3) ◽  
pp. 793-811 ◽  
Author(s):  
David B. Friedman ◽  
Salisha Hill ◽  
Jeffrey W. Keller ◽  
Nipun B. Merchant ◽  
Shawn E. Levy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Colon Cancer ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Human Colon ◽  
Two Dimensional ◽  
Human Colon Cancer ◽  
Difference Gel Electrophoresis

Proteome analysis of human hepatocellular carcinoma tissues by two-dimensional difference gel electrophoresis and mass spectrometry

PROTEOMICS ◽  
2005 ◽  
Vol 5 (8) ◽  
pp. 2258-2271 ◽  
Author(s):  
Cynthia R. M. Y. Liang ◽  
Chon Kar Leow ◽  
Jason C. H. Neo ◽  
Gek San Tan ◽  
Siaw Ling Lo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Hepatocellular Carcinoma ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Human Hepatocellular Carcinoma ◽  
Two Dimensional ◽  
Difference Gel Electrophoresis

Proteome analysis of round-headed sperm by two-dimensional difference gel electrophoresis and mass spectrometry

2008 ◽  
Vol 32 (3) ◽  
pp. S43-S44
Author(s):  
Ting Ting Liao ◽  
Li Qing Fan ◽  
Guang Xiu Lu ◽  
Wen Bing Zhu ◽  
Ling Wei Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Difference Gel Electrophoresis

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

scholarly journals Proteome Analysis of Hepatocellular Carcinoma by Two-dimensional Difference Gel Electrophoresis

2007 ◽  
Vol 6 (10) ◽  
pp. 1798-1808 ◽  
Author(s):  
Wei Sun ◽  
Baocai Xing ◽  
Yi Sun ◽  
Xiaojuan Du ◽  
Min Lu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Difference Gel Electrophoresis

Proteome analysis of Leishmania (Viannia) braziliensis by two-dimensional gel electrophoresis and mass spectrometry

2007 ◽  
Vol 154 (1) ◽  
pp. 6-21 ◽  
Author(s):  
Patricia Cuervo ◽  
Jose Batista de Jesus ◽  
Magno Junqueira ◽  
Leila Mendonça-Lima ◽  
Luis Javier González ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis
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