Dissecting DNA-protein and protein-protein interactions involved in bacterial transcriptional regulation by a sensitive protein array method combining a near-infrared fluorescence detection

PROTEOMICS ◽  
2003 ◽  
Vol 3 (5) ◽  
pp. 647-657 ◽  
Author(s):  
Marina Snapyan ◽  
Michèle Lecocq ◽  
Laetitia Guével ◽  
Marie-Claire Arnaud ◽  
Anahit Ghochikyan ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Fluorescence Detection ◽  
Protein Interactions ◽  
Near Infrared ◽  
Protein Array ◽  
Protein Protein Interactions ◽  
Near Infrared Fluorescence

scholarly journals TRI_tool: a web-tool for prediction of protein–protein interactions in human transcriptional regulation

2016 ◽  
Vol 33 (2) ◽  
pp. 289-291 ◽  
Author(s):  
Vladimir Perovic ◽  
Neven Sumonja ◽  
Branislava Gemovic ◽  
Eneda Toska ◽  
Stefan G. Roberts ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Web Tool

Distance Controlled and Electrically Driven Photoluminescence Quench From Quantum Dot-Au Complexes

2010 ◽  
Vol 1257 ◽  
Author(s):  
Zhitao Kang ◽  
Jie Xu ◽  
Dinal Andreasen ◽  
Brent Karl Wagner
Keyword(s):  
Gold Nanoparticles ◽  
Protein Interactions ◽  
Near Infrared ◽  
Incident Light ◽  
Gold Surface ◽  
Electric Signal ◽  
Protein Protein Interactions ◽  
Quenching Effect ◽  
Quenching Efficiency ◽  
Pl Quenching

AbstractQuantum Dots (QDs) bound to gold nanoparticles have shown photoluminescence (PL) quenching dependent on distance between the two particles. The incident light from the QD couples to plasmon excitation of the metal when the frequencies of the light and the surface plasmon resonance (SPR) coincide, leading to a reduction in emitted PL in the system. The quenching effect of gold nanoparticles on QDs was used to study protein-protein interactions with the potential for drug screening applications. CdTe and CdHgTe QDs with emission wavelengths from 500˜900nm were synthesized and gold nanospheres and nanorods with controlled absorption in the visible and near-infrared (NIR) wavelength regions were prepared. The PL quenching of QD-Protein-Protein-Au complexes was studied as a function of Au concentration, QD size and protein type. A quenching efficiency of up to 90% was observed. The QD-Au complexes were also studied for electric potential sensing. The surface of the QDs was negatively charged due to thiol ligands capping. By applying a positive potential on the gold or gold nanoparticle attached substrate, the local electric field between the substrate and the statically charged QDs would pull the QDs closer to the gold surface and quench the QD PL. PL quenching of QD with Au was studied as a function of electric signal and QD type. In this methodology, electric signals were effectively converted to optical signals.

Augmented microscopy with near-infrared fluorescence detection

2015 ◽  
Author(s):  
Jeffrey R. Watson ◽  
Nikolay Martirosyan ◽  
Jesse Skoch ◽  
G. Michael Lemole ◽  
Rein Anton ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Near Infrared ◽  
Near Infrared Fluorescence
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