scholarly journals Differential abundance proteins associated with rapid growth of etiolated coleoptiles in maize

Plant Direct ◽  
2021 ◽  
Vol 5 (6) ◽  
Author(s):  
Liangjie Niu ◽  
Weili Huang ◽  
Lunyu Liu ◽  
Chenhui Xu ◽  
Xiaolin Wu ◽  
...  
Keyword(s):  
Rapid Growth ◽  
Differential Abundance ◽  
Abundance Proteins

scholarly journals Entamoeba histolytica: Proteomics Bioinformatics Reveal Predictive Functions and Protein–Protein Interactions of Differentially Abundant Membrane and Cytosolic Proteins

Membranes ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 376
Author(s):  
Norhidayah Azmi ◽  
Nurulhasanah Othman
Keyword(s):  
Entamoeba Histolytica ◽  
Protein Interactions ◽  
Biological Process ◽  
Protein Protein Interactions ◽  
Differential Abundance ◽  
Cytosolic Proteins ◽  
Functional Annotations ◽  
Predictive Functions ◽  
Differentially Abundant Proteins ◽  
Abundance Proteins

Amoebiasis is caused by Entamoeba histolytica and ranked second for parasitic diseases causing death after malaria. E. histolytica membrane and cytosolic proteins play important roles in the pathogenesis. Our previous study had shown several cytosolic proteins were found in the membrane fraction. Therefore, this study aimed to quantify the differential abundance of membrane and cytosolic proteins in membrane versus cytosolic fractions and analyze their predicted functions and interaction. Previous LC-ESI-MS/MS data were analyzed by PERSEUS software for the differentially abundant proteins, then they were classified into their functional annotations and the protein networks were summarized using PantherDB and STRiNG, respectively. The results showed 24 (44.4%) out of the 54 proteins that increased in abundance were membrane proteins and 30 were cytosolic proteins. Meanwhile, 45 cytosolic proteins were found to decrease in abundance. Functional analysis showed differential abundance proteins involved in the molecular function, biological process, and cellular component with 18.88%, 33.04% and, 48.07%, respectively. The STRiNG server predicted that the decreased abundance proteins had more protein–protein network interactions compared to increased abundance proteins. Overall, this study has confirmed the presence of the differentially abundant membrane and cytosolic proteins and provided the predictive functions and interactions between them.

scholarly journals Quantitative Proteomics Analysis of Lettuce (Lactuca sativa L.) Reveals Molecular Basis-Associated Auxin and Photosynthesis with Bolting Induced by High Temperature

2018 ◽  
Vol 19 (10) ◽  
pp. 2967 ◽  
Author(s):  
Jing-Hong Hao ◽  
Li-Li Zhang ◽  
Pan-Pan Li ◽  
Yan-Chuan Sun ◽  
Jian-Ke Li ◽  
...  
Keyword(s):  
High Temperature ◽  
Lactuca Sativa ◽  
Molecular Basis ◽  
Tryptophan Metabolism ◽  
Proteomics Analysis ◽  
Differential Abundance ◽  
Control Temperature ◽  
Lactuca Sativa L ◽  
Iaa Biosynthesis ◽  
Abundance Proteins

Bolting is a key process in the growth and development of lettuce (Lactuca sativa L.). A high temperature can induce early bolting, which decreases both the quality and production of lettuce. However, knowledge of underlying lettuce bolting is still lacking. To better understand the molecular basis of bolting, a comparative proteomics analysis was conducted on lettuce stems, during the bolting period induced by a high temperature (33 °C) and a control temperature (20 °C) using iTRAQ-based proteomics, phenotypic measures, and biological verifications using qRT-PCR and Western blot. The high temperature induced lettuce bolting, while the control temperature did not. Of the 5454 identified proteins, 619 proteins presented differential abundance induced by high-temperature relative to the control group, of which 345 had an increased abundance and 274 had a decreased abundance. Proteins with an abundance level change were mainly enriched in pathways associated with photosynthesis and tryptophan metabolism involved in auxin (IAA) biosynthesis. Moreover, among the proteins with differential abundance, proteins associated with photosynthesis and tryptophan metabolism were increased. These findings indicate that a high temperature enhances the function of photosynthesis and IAA biosynthesis to promote the process of bolting, which is in line with the physiology and transcription level of IAA metabolism. Our data provide a first comprehensive dataset for gaining novel understanding of the molecular basis underlying lettuce bolting induced by high temperature. It is potentially important for further functional analysis and genetic manipulation for molecular breeding to breed new cultivars of lettuce to restrain early bolting, which is vital for improving vegetable quality.

scholarly journals TMT-Based Quantitative Proteomic Analysis Identification of Integrin Alpha 3 and Integrin Alpha 5 as Novel Biomarkers in Pathogenesis of Acute Aortic Dissection

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Lingyu Xing ◽  
Yuan Xue ◽  
Yilin Yang ◽  
Ping Wu ◽  
Catherine C. L. Wong ◽  
...  
Keyword(s):  
Aortic Dissection ◽  
Aortic Arch ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Acute Aortic Dissection ◽  
High Rate ◽  
Differential Abundance ◽  
Healthy Donors ◽  
Novel Biomarkers ◽  
Abundance Proteins

Background. Acute aortic dissection (AAD) is a devastating cardiovascular disease with a high rate of disability and mortality. This disease often rapidly progresses to fatal multiple organ hypoperfusion, and the incidence has been increasing in recent years. However, the molecular mechanisms have yet to be clarified. This study is aimed at identifying the differential abundance proteins (DAPs) of aortic arch tissues in patients with AAD by proteomics and select possible proteins involved in AAD pathogenesis. Methods. The fresh aortic arch tissues obtained from 5 AAD patients and 1 healthy donor were analyzed by amine-reactive tandem mass tag (TMT) labelling and mass spectrometry; then, the pathological sections of another 10 healthy donors and 20 AAD patients were chosen to verify the proteomic results by immunohistochemistry. Results. Of 809 proteins identified by proteomic analysis, 132 differential abundance proteins (DAPs) were screened, of which 100 proteins were significantly downregulated while 32 upregulated. Among 100 downregulated proteins, two proteins with known function, integrin alpha 3 (ITGA-3) and ITGA-5, were selected as target proteins involved in AAD pathogenesis. Two target DAPs were verified by immunohistochemisty, and the results showed that the integrated option density (IOD) of ITGA-3 and ITGA-5 in AAD patients was significantly lower than that in healthy donors, which were consistent with the proteomic results (P<0.001). Conclusion. ITGA-3 and ITGA-5 represent novel biomarkers for the pathogenesis of AAD and might be a therapeutic target in the future.

Removing Substrate Background in TEM Microanalysis

1974 ◽  
Vol 32 ◽  
pp. 568-569
Author(s):  
John C. Russ ◽  
Nicholas C. Barbi
Keyword(s):  
Electrical Conductivity ◽  
Rapid Growth ◽  
Organic Film ◽  
Absolute Concentration ◽  
Background Signal ◽  
X Ray ◽  
Elemental Concentrations ◽  
Transmission Electron ◽  
Electron Microscopes ◽  
The Continuum

The rapid growth of interest in attaching energy-dispersive x-ray analysis systems to transmission electron microscopes has centered largely on microanalysis of biological specimens. These are frequently either embedded in plastic or supported by an organic film, which is of great importance as regards stability under the beam since it provides thermal and electrical conductivity from the specimen to the grid.Unfortunately, the supporting medium also produces continuum x-radiation or Bremsstrahlung, which is added to the x-ray spectrum from the sample. It is not difficult to separate the characteristic peaks from the elements in the specimen from the total continuum background, but sometimes it is also necessary to separate the continuum due to the sample from that due to the support. For instance, it is possible to compute relative elemental concentrations in the sample, without standards, based on the relative net characteristic elemental intensities without regard to background; but to calculate absolute concentration, it is necessary to use the background signal itself as a measure of the total excited specimen mass.

PERLINDUNGAN HUKUM TERHADAP KARYA SENI BATIK YANG BELUM TERDAFTAR MENURUT UNDANG-UNDANG NOMOR 28 TAHUN 2014 TENTANG HAK CIPTA

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Inggrit Fernandes
Keyword(s):  
Intellectual Property ◽  
Property Rights ◽  
Cultural Heritage ◽  
Intellectual Property Rights ◽  
Rapid Growth ◽  
Economic Value ◽  
Legal Protection ◽  
Property Law ◽  
Market Demand ◽  
Work Of Art

Batik artwork is one of the treasures of the nation's cultural heritage. Batik artwork is currently experiencing rapid growth. The amount of interest and market demand for this art resulted batik artwork became one of the commodities in the country and abroad. Thus, if the batik artwork is not protected then the future can be assured of a new conflict arises in the realm of intellectual property law. Act No. 28 of 2014 on Copyright has accommodated artwork batik as one of the creations that are protected by law. So that this work of art than as a cultural heritage also have economic value for its creator. Then how the legal protection of the batik artwork yaang not registered? Does this also can be protected? While in the registration of intellectual property rights is a necessity so that it has the force of law to the work produced

Sharade Marathe. Regulation and Development: The Indian Policy Experience of Controls Over Industry. New Delhi: Sage Publications India Pvt. Ltd. 1986. 328 pp.Price: (Hardbound edition) Rupees (Indian) 195.00.

1987 ◽  
Vol 26 (2) ◽  
pp. 225-227
Author(s):  
Khwaja Sarmad
Keyword(s):  
Industrial Policy ◽  
Rapid Growth ◽  
Industrial Structure ◽  
Industrial Sector ◽  
Primary Objective ◽  
Indian Policy ◽  
New Delhi ◽  
The Past ◽  
Distinguished Career ◽  
Comprehensive Manner

This book documents in a comprehensive manner the 'twists and turns' in India's industrial policy and strongly suggests the need for a re-orientation of this policy to overcome the weaknesses in the industrial structure and to utilize the sources of its strength. The author has had a distinguished career in the Indian Economic Service and brings this experience to bear on his analysis of the evolution of industrial policy in India. In India, the primary objective of planned development has been the creation of a technologically mature society capable of sustaining a process of self-propelled growth without extreme concentration of wealth in a few hands. It is rightly pointed out in the book that this objective is possible only in the context of rapid growth, which is the ultimate test of industrial policy. The book traces the origins of India's industrial policy and analyses its evolution during the past thirty years, showing how there has been an increasing gap between the objectives of this policy and the performance of the industrial sector.

Smart Cities: Necessity and Development Strategies

2020 ◽  
Vol 15 (2) ◽  
pp. 52-58
Author(s):  
L. V. Shkvarya ◽  
A. S. Semenov
Keyword(s):  
Economic Development ◽  
Rapid Growth ◽  
Smart City ◽  
Smart Cities ◽  
First Century ◽  
Quality Improvements ◽  
Urban Settlement ◽  
Life Problems ◽  
Socio Economic Development ◽  
High Technologies

In the twenty-first century different countries and cities are increasingly seeking to introduce quality improvements in their livelihoods, generate for its residents an environment that is called “smart city” on the basis of high technologies. The article shows that the emergence of a “smart” city is an objective necessity due to the rapid growth of cities in the present and in the future. “Smart” city is designed to solve life problems in cities and create conditions for the socio-economic development of cities and countries, and for a favorable stay of residents on its territory. There are a number of strategies to implement the concept of “smart”, but it is important for each urban settlement to develop its own approaches and projects.

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