Cibacron Blue F3GA-attached magnetic poly(2-hydroxyethyl methacrylate) beads for human serum albumin adsorption

2004 ◽  
Vol 53 (3) ◽  
pp. 332-338 ◽  
Author(s):  
Mehmet Odabaşı ◽  
Adil Denizli
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Hydroxyethyl Methacrylate ◽  
Cibacron Blue ◽  
Albumin Adsorption ◽  
Cibacron Blue F3ga

Chromatographic profiles of cyanogen bromide fragments of unreduced human serum albumin on immobilized Cibacron Blue F3G-A

1993 ◽  
Vol 639 (2) ◽  
pp. 341-345 ◽  
Author(s):  
Anna Compagnini ◽  
Maria Fichera ◽  
Salvatore Fisichella ◽  
Salvatore Foti ◽  
Rosaria Saletti
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Cyanogen Bromide ◽  
Cibacron Blue ◽  
Chromatographic Profiles

Human serum albumin binding to silica nanoparticles – effect of protein fatty acid ligand

2014 ◽  
Vol 16 (21) ◽  
pp. 10157-10168 ◽  
Author(s):  
Joo Chuan Ang ◽  
Mark J. Henderson ◽  
Richard A. Campbell ◽  
Jhih-Min Lin ◽  
Peter N. Yaron ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Time Dependence ◽  
Human Serum ◽  
Silica Nanoparticles ◽  
Albumin Binding ◽  
Acid Ligand ◽  
Human Serum Albumin Binding ◽  
Albumin Adsorption

Fat containing and defatted human serum albumin adsorption to silica nanoparticles have different structures and time dependence to form.

scholarly journals The effect of ligand presaturation on the interaction of serum albumins with an immobilized Cibacron Blue 3G-A studied by affinity gel electrophoresis

1981 ◽  
Vol 199 (3) ◽  
pp. 465-472 ◽  
Author(s):  
E C Metcalf ◽  
B Crow ◽  
P D G Dean
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Dissociation Constant ◽  
Gel Electrophoresis ◽  
Dissociation Constants ◽  
Serum Albumins ◽  
Albumin Ratio ◽  
Cibacron Blue ◽  
Affinity Gel Electrophoresis

The interaction of the immobilized triazine dye Cibacron Blue 3G-A with rat, rabbit, sheep, goat, bovine and human serum albumins was studied by affinity gel electrophoresis. Dissociation constants were estimated in each instance and showed human serum albumin to have a significantly higher affinity for the dye than did albumin from any other species. Pretreatment of the defatted proteins with bilirubin (3 mol of bilirubin/mol of protein) did not increase the dissociation constants of the serum albumins, whereas pretreatment with palmitate (7 mol of palmitate/mol of protein) increased the dissociation constant in all cases: 3-fold for human serum albumin, 15-fold for other serum albumins. Increasing the bilirubin/albumin ratio (to 7:1) did not affect the dissociation constant of the albumins studied. Decreasing the palmitate/albumin ratio decreased the dissociation constant for human serum albumin, but did not affect those of bovine and rat albumins. Altering the chain length of the presaturating fatty acid dramatically changed the dissociation constant of both human and bovine serum albumins. Butyrate, hexanoate, octanoate and decanoate did not significantly influence the dissociation constants of bovine and human serum albumins for Cibacron Blue, whereas laurate, myristate and palmitate greatly increased the dissociation constant. These data are discussed in relationship to the behaviour of albumins during dye--agarose column chromatography. In Addendum the effect of nucleotide presaturation on the interaction between Bacillus stearothermophilus 6-phosphogluconate dehydrogenase and the immobilized triazine dyes Cibacron Blue 3G-A and Procion Red HE-3B was examined, and the implications for dye--ligand chromatography are discussed.

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