Characterization of polymer composites by electron microprobe analysis

1972 ◽  
Vol 12 (1) ◽  
pp. 34-40 ◽  
Author(s):  
S. S. Labana ◽  
H. K. Plummer ◽  
W. J. Burlant
Keyword(s):  
Polymer Composites ◽  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis

scholarly journals CHEMICAL CHARACTERIZATION OF UNCOATED ASBESTOS FIBERS FROM THE LUNGS OF ASBESTOS WORKERS BY ELECTRON MICROPROBE ANALYSIS

1972 ◽  
Vol 20 (9) ◽  
pp. 735-740 ◽  
Author(s):  
ARTHUR M. LANGER ◽  
IVAN B. RUBIN ◽  
IRVING J. SELIKOFF ◽  
FRED D. POOLEY
Keyword(s):  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Chemical Characterization ◽  
Magnesium Content ◽  
Asbestos Fibers ◽  
Magnesium Loss ◽  
Bulk Chemistry ◽  
Asbestos Body

Lung tissues have been obtained from workmen with defined asbestos fiber exposure. These tissues have been prepared by the carbon extraction technique and examined with the electron microprobe analyzer. The uncoated fibers present in these specimens have been chemically characterized and compared with standard reference asbestos samples. The bulk chemistry of the fibers observed in lung tissues is similar to that of the reference fibers so that identification may be made. However, a statistical analysis of the measured emission characteristics from anthophyllite and amosite indicates that some magnesium loss has taken place. This loss appears to correlate with the magnesium content of the fibers. A slight iron increase was also noted in the same fibers, probably related to an incipient asbestos body formation.

Scanning Electron Microscopy and Electron Microprobe Analysis of Malignant Cell Cultures

1968 ◽  
Vol 26 ◽  
pp. 164-165
Author(s):  
R. I. Johnsson-Hegyeli ◽  
A. F. Hegyeli ◽  
D. K. Landstrom ◽  
W. C. Lane
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Cultures ◽  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Three Dimensional ◽  
Malignant Cell ◽  
Interference Microscopy ◽  
Scanning Electron

Last year we reported on the use of reflected light interference microscopy (RLIM) for the direct color photography of the surfaces of living normal and malignant cell cultures without the use of replicas, fixatives, or stains. The surface topography of living cells was found to follow underlying cellular structures such as nuceloli, nuclear membranes, and cytoplasmic organelles, making possible the study of their three-dimensional relationships in time. The technique makes possible the direct examination of cells grown on opaque as well as transparent surfaces. The successful in situ electron microprobe analysis of the elemental composition and distribution within single tissue culture cells was also reported.This paper deals with the parallel and combined use of scanning electron microscopy (SEM) and the two previous techniques in a study of living and fixed cancer cells. All three studies can be carried out consecutively on the same experimental specimens without disturbing the cells or their structural relationships to each other and the surface on which they are grown. KB carcinoma cells were grown on glass coverslips in closed Leighto tubes as previously described. The cultures were photographed alive by means of RLIM, then fixed with a fixative modified from Sabatini, et al (1963).

Sign in / Sign up

Export Citation Format

Share Document