Fabrication and evaluation of homogeneous alginate/polyacrylamide–chitosan–gelatin composite hydrogel scaffolds based on the interpenetrating networks for tissue engineering

2021 ◽  
Author(s):  
Xiuqiong Chen ◽  
Huiqiong Yan ◽  
Chaoling Bao ◽  
Qingmei Zhu ◽  
Zhaowen Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Composite Hydrogel ◽  
Interpenetrating Networks ◽  
Hydrogel Scaffolds

Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells

Biomaterials ◽  
2014 ◽  
Vol 35 (6) ◽  
pp. 1914-1923 ◽  
Author(s):  
Hoi Ki Cheung ◽  
Tim Tian Y. Han ◽  
Dale M. Marecak ◽  
John F. Watkins ◽  
Brian G. Amsden ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Soft Tissue ◽  
Composite Hydrogel ◽  
Adipose Derived Stem Cells ◽  
Hydrogel Scaffolds ◽  
Soft Tissue Engineering

scholarly journals Long-Term Cryostorage of Mesenchymal Stem Cell-Containing Hybrid Hydrogel Scaffolds Based on Fibrin and Collagen

Gels ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 44
Author(s):  
Marfa N. Egorikhina ◽  
Yulia P. Rubtsova ◽  
Diana Ya. Aleynik
Keyword(s):  
Tissue Engineering ◽  
Proliferative Activity ◽  
Experimental Protocol ◽  
Hydrogel Scaffold ◽  
Hybrid Hydrogel ◽  
Hydrogel Scaffolds ◽  
High Viability ◽  
Scaffold Structure ◽  
Difficult Issue

The most difficult issue when using tissue engineering products is enabling the ability to store them without losing their restorative capacity. The numbers and viability of mesenchymal stem cells encapsulated in a hydrogel scaffold after cryostorage at −80 °C (by using, individually, two kinds of cryoprotectors—Bambanker and 10% DMSO (Dimethyl sulfoxide) solution) for 3, 6, 9, and 12 months were determined, with subsequent assessment of cell proliferation after 96 h. The analysis of the cellular component was performed using fluorescence microscopy and the two fluorochromes—Hoechst 3334 and NucGreenTM Dead 488. The experimental protocol ensured the preservation of cells in the scaffold structure, retaining both high viability and proliferative activity during storage for 3 months. Longer storage of scaffolds led to their significant changes. Therefore, after 6 months, the proliferative activity of cells decreased. Cryostorage of scaffolds for 9 months led to a decrease in cells’ viability and proliferative activity. As a result of cryostorage of scaffolds for 12 months, a decrease in viability and proliferative activity of cells was observed, as well as pronounced changes in the structure of the hydrogel. The described scaffold cryostorage protocol could become the basis for the development of storage protocols for such tissue engineering products, and for helping to extend the possibilities of their clinical use while accelerating their commercialization.

scholarly journals 14-3-3ε protein-loaded 3D hydrogels favor osteogenesis

2020 ◽  
Vol 31 (11) ◽  
Author(s):  
Ana A. Aldana ◽  
Marina Uhart ◽  
Gustavo A. Abraham ◽  
Diego M. Bustos ◽  
Aldo R. Boccaccini
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
3D Printing ◽  
Osteogenic Differentiation ◽  
Alginate Hydrogel ◽  
Hydrogel Scaffolds ◽  
Future Developments ◽  
Positive Effect ◽  
Cell Adhesion And Proliferation

Abstract3D printing has emerged as vanguard technique of biofabrication to assemble cells, biomaterials and biomolecules in a spatially controlled manner to reproduce native tissues. In this work, gelatin methacrylate (GelMA)/alginate hydrogel scaffolds were obtained by 3D printing and 14-3-3ε protein was encapsulated in the hydrogel to induce osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASC). GelMA/alginate-based grid-like structures were printed and remained stable upon photo-crosslinking. The viscosity of alginate allowed to control the pore size and strand width. A higher viscosity of hydrogel ink enhanced the printing accuracy. Protein-loaded GelMA/alginate-based hydrogel showed a clear induction of the osteogenic differentiation of hASC cells. The results are relevant for future developments of GelMA/alginate for bone tissue engineering given the positive effect of 14-3-3ε protein on both cell adhesion and proliferation.

scholarly journals Cyclic tensile stimulation enrichment of Schwann cell-laden auxetic hydrogel scaffolds towards peripheral nerve tissue engineering

2020 ◽  
Vol 195 ◽  
pp. 108982 ◽  
Author(s):  
Yi-Wen Chen ◽  
Kan Wang ◽  
Chia-Che Ho ◽  
Chia-Tze Kao ◽  
Hooi Yee Ng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Tissue ◽  
Hydrogel Scaffolds ◽  
Nerve Tissue Engineering

Co-delivery of Simvastatin and Demineralized Bone Matrix Hierarchically from Nanosheet-based Supramolecular Hydrogels for Osteogenesis

2021 ◽  
Author(s):  
Xiao Zhang ◽  
Jiabing Fan ◽  
Chen Chen ◽  
Tara Aghaloo ◽  
Min Lee
Keyword(s):  
Tissue Engineering ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Therapeutic Agents ◽  
Engineering Applications ◽  
3D Scaffolds ◽  
Hydrogel Scaffolds ◽  
Delivery Platform ◽  
Weak Compatibility ◽  
Demineralized Bone

Supramolecular hydrogels are widely used as 3D scaffolds and delivery platform in tissue engineering applications. However, hydrophobic therapeutic agents exhibit weak compatibility in hydrogel scaffolds along with aggregation and precipitate....

scholarly journals Tuning the Properties of PNIPAm-Based Hydrogel Scaffolds for Cartilage Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3154
Author(s):  
Md Mohosin Rana ◽  
Hector De la Hoz Siegler
Keyword(s):  
Tissue Engineering ◽  
Physical Properties ◽  
Tissue Regeneration ◽  
Structural Integrity ◽  
Cartilage Tissue ◽  
Design Parameters ◽  
Comprehensive Overview ◽  
Hydrogel Scaffolds ◽  
Design Variables

Poly(N-isopropylacrylamide) (PNIPAm) is a three-dimensional (3D) crosslinked polymer that can interact with human cells and play an important role in the development of tissue morphogenesis in both in vitro and in vivo conditions. PNIPAm-based scaffolds possess many desirable structural and physical properties required for tissue regeneration, but insufficient mechanical strength, biocompatibility, and biomimicry for tissue development remain obstacles for their application in tissue engineering. The structural integrity and physical properties of the hydrogels depend on the crosslinks formed between polymer chains during synthesis. A variety of design variables including crosslinker content, the combination of natural and synthetic polymers, and solvent type have been explored over the past decade to develop PNIPAm-based scaffolds with optimized properties suitable for tissue engineering applications. These design parameters have been implemented to provide hydrogel scaffolds with dynamic and spatially patterned cues that mimic the biological environment and guide the required cellular functions for cartilage tissue regeneration. The current advances on tuning the properties of PNIPAm-based scaffolds were searched for on Google Scholar, PubMed, and Web of Science. This review provides a comprehensive overview of the scaffolding properties of PNIPAm-based hydrogels and the effects of synthesis-solvent and crosslinking density on tuning these properties. Finally, the challenges and perspectives of considering these two design variables for developing PNIPAm-based scaffolds are outlined.

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