A novel pericentric inversion of chromosome 14 involving the rRNA gene cluster

2005 ◽  
Vol 25 (7) ◽  
pp. 620-621 ◽  
Author(s):  
Natalia T Leach ◽  
Suzanne M Cole ◽  
Deborah J Sandstrom ◽  
Stanislawa Weremowicz
Keyword(s):  
Gene Cluster ◽  
Pericentric Inversion ◽  
Rrna Gene ◽  
Chromosome 14

Balanced and unbalanced pericentric inversion of a chromosome 14

1978 ◽  
Vol 43 (1) ◽  
pp. 103-106 ◽  
Author(s):  
R. A. Pfeiffer ◽  
E. Kessel
Keyword(s):  
Pericentric Inversion ◽  
Chromosome 14

scholarly journals Direct and Rapid Detection by PCR ofErysipelothrix sp. DNAs Prepared from Bacterial Strains and Animal Tissues

1999 ◽  
Vol 37 (12) ◽  
pp. 4093-4098 ◽  
Author(s):  
Kouichi Takeshi ◽  
Souichi Makino ◽  
Tetsuya Ikeda ◽  
Noriko Takada ◽  
Atsushi Nakashiro ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gene Cluster ◽  
Dna Sequences ◽  
Screening Method ◽  
Rapid Screening ◽  
Rrna Genes ◽  
23S Rrna ◽  
Rrna Gene ◽  
Link Type ◽  
Species Specific

A PCR method for rapid screening of Erysipelothrix spp. in the slaughterhouse was carried out by using four species-specific sets of oligonucleotide primers after initial amplification with the primer set MO101-MO102, which amplifies the 16S rRNA sequences of all four Erysipelothrix species. The DNA sequences coding for the rRNA gene cluster, including 16S rRNA, 23S rRNA, and the noncoding region downstream of 5S rRNA, were determined in order to design primers for the species-specific PCR detection system. The homology among the 4.5-kb DNA sequences of the rRNA genes ofErysipelothrix rhusiopathiae serovar 2 (DNA Data Bank of Japan accession no. AB019247), E. tonsillarum serovar 7 (accession no. AB019248), E. rhusiopathiae serovar 13 (accession no. AB019249), and E. rhusiopathiae serovar 18 (accession no. AB019250) ranged from 96.0 to 98.4%. The PCR amplifications were specific and were able to distinguish the DNAs from each of the four Erysipelothrix species. The results of PCR tests performed directly with tissue specimens from diseased animals were compared with the results of cultivation tests, and the PCR tests were completed within 5 h. The test with this species-specific system based on PCR amplification with the DNA sequences coding for the rRNA gene cluster was an accurate, easy-to-read screening method for rapid diagnosis of Erysipelothrix sp. infection in the slaughterhouse.

Genetic mapping of the 5S rRNA gene cluster of the nematode Caenorhabditis elegans

1986 ◽  
Vol 28 (4) ◽  
pp. 545-553 ◽  
Author(s):  
D. W. Nelson ◽  
B. M. Honda
Keyword(s):  
Caenorhabditis Elegans ◽  
Linkage Group ◽  
Gene Cluster ◽  
5S Rrna ◽  
Rrna Gene ◽  
Nematode Caenorhabditis Elegans ◽  
Group V ◽  
Length Difference ◽  
5S Rrna Gene ◽  
Restriction Fragment Length

We have identified a restriction fragment length difference (RFLD) affecting the genomic sequences immediately flanking the 5S rRNA gene cluster in the Bristol and Bergerac strains of the nematode Caenorhabditis elegans. We have used this RFLD as a molecular marker to follow the segregation of the 5S rRNA gene cluster through a series of two- and three-factor interstrain crosses. Our results show that the 5S rRNA gene cluster maps between unc-76 and dpy-21 on the right arm of linkage group V. This genetic localization provides a linkage group V "landmark" with which to localize other cloned sequences by in situ hybridization.Key words: Caenorhabditis elegans, 5S rRNA gene cluster, restriction fragment length difference, genetic mapping.

Transcription of the rRNA gene cluster in Aspergillus nidulanss

1983 ◽  
Vol 7 (2) ◽  
pp. 113-115 ◽  
Author(s):  
Ewa Bartnik ◽  
Piotr Borsuk
Keyword(s):  
Gene Cluster ◽  
Rrna Gene

scholarly journals Candicidin Biosynthesis Gene Cluster Is Widely Distributed among Streptomyces spp. Isolated from the Sediments and the Neuston Layer of the Trondheim Fjord, Norway

2009 ◽  
Vol 75 (10) ◽  
pp. 3296-3303 ◽  
Author(s):  
Hanne J�rgensen ◽  
Espen Fj�rvik ◽  
Sigrid Hakv�g ◽  
Per Bruheim ◽  
Harald Bredholt ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Blot Hybridization ◽  
Morphological Diversity ◽  
Southern Blot Hybridization ◽  
Rrna Gene ◽  
Uv Spectrum ◽  
Biosynthesis Gene Cluster ◽  
Streptomyces Spp ◽  
Biosynthesis Gene ◽  
Large Plasmids

ABSTRACT A large number of Streptomyces bacteria with antifungal activity isolated from samples collected in the Trondheim fjord (Norway) were found to produce polyene compounds. Investigation of polyene-containing extracts revealed that most of the isolates produced the same compound, which had an atomic mass and UV spectrum corresponding to those of candicidin D. The morphological diversity of these isolates prompted us to speculate about the involvement of a mobile genetic element in dissemination of the candicidin biosynthesis gene cluster (can). Eight candicidin-producing isolates were analyzed by performing a 16S rRNA gene-based taxonomic analysis, pulsed-field gel electrophoresis, PCR, and Southern blot hybridization with can-specific probes. These analyses revealed that most of the isolates were related, although they were morphologically diverse, and that all of them contained can genes. The majority of the isolates studied contained large plasmids, and two can-specific probes hybridized to a 250-kb plasmid in one isolate. Incubation of the latter isolate at a high temperature resulted in loss of the can genes and candicidin production, while mating of the “cured” strain with a plasmid-containing donor restored candicidin production. The latter result suggested that the 250-kb plasmid contains the complete can gene cluster and could be responsible for conjugative transfer of this cluster to other streptomycetes.

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