scholarly journals Application of Box–Behnken design for ultrasound‐assisted extraction and recycling preparative HPLC for isolation of anthraquinones from Cassia singueana

2018 ◽  
Vol 30 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Saidu Jibril ◽  
Norazah Basar ◽  
Hasnah Mohd Sirat ◽  
Roswanira Abdul Wahab ◽  
Naji Arafat Mahat ◽  
...  
Keyword(s):  
Preparative Hplc ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals Optimization of a Green Ultrasound-Assisted Extraction of Different Polyphenols from Pistacia lentiscus L. Leaves Using a Response Surface Methodology

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1482
Author(s):  
Cassandra Detti ◽  
Luana Beatriz dos Santos Nascimento ◽  
Cecilia Brunetti ◽  
Francesco Ferrini ◽  
Antonella Gori
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Industrial Applications ◽  
Pistacia Lentiscus ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Time Decrease

Pistacia lentiscus leaves are used in several applications, thanks to their polyphenolic abundance. Thiswork aimed to characterize the polyphenols and to optimize the extraction conditions to shorten the time, decrease the consumption of solvent, and to maximize the yield of different classes of phenolics, which have diverse industrial applications. The variables were optimized by applying a Box–Behnken design. Galloyl and myricetin derivatives were the most abundant compounds, and two new tetragalloyl derivatives were identified by LC-MS/MS. According to the models, the maximum yields of polyphenols (51.3 ± 1.8 mg g−1 DW) and tannins (40.2 ± 1.4 mg g−1 DW) were obtained using 0.12 L g−1 of 40% ethanol at 50 °C. The highest content of flavonoids (10.2 ± 0.8 mg g−1 DW) was obtained using 0.13 L g−1 of 50% ethanol at 50 °C, while 0.1 L g−1 of 30% ethanol at 30 °C resulted in higher amounts of myricitrin (2.6 ± 0.19 mg g−1 DW). Our optimized extraction decreased the ethanolic fraction by 25% and halved the time compared to other methods. These conditions can be applied differently to obtain P. lentiscus extracts richer in tannins or flavonoids, which might be employed for various purposes.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

2019 ◽  
Vol 92 (3) ◽  
pp. 369-377
Author(s):  
Barbara Fumić ◽  
Mario Jug ◽  
Marijana Zovko Končić
Keyword(s):  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Lotus Corniculatus ◽  
Extraction Yield ◽  
Phenolic Antioxidants ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Ultrasound-assisted extraction of phenolic antioxidants from Lotus corniculatus was optimized using response surface methodology. The extraction was performed according to the Box–Behnken design with ethanol concentration, temperature, and pH, as independent variables. The responses were extraction yield, DPPH radical scavenging activity (RSA) IC50 and content of different phenolic compounds (total phenols, flavonoids and phenolic acids, as well as quercetin, kaempferol and genistein derivatives). The models were used to calculate best conditions for maximal extraction of phenolic compounds and antiradical activity. Use of the optimized extraction parameters increased the content of quercetin and kaempferol derivatives more than tenfold (from 6.07 to 65.10 mg mL–1 and 6.69 to 92.75 mg mL–1, respectively). The results of this work stress the importance of careful selection of conditions for flavonoids extraction. Abundance of bioactive phenolics in L. corniculatus extracts obtained under optimized extraction conditions opens the possibility for wider utilization of this plant.

scholarly journals Optimization of ultrasound - assisted extraction of phenolic compounds from wood apple pulp: Identification of phytochemicals using GC-MS

2019 ◽  
Vol 25 (4) ◽  
pp. 361-368
Author(s):  
Anbarasi Karunanithi ◽  
Sivakumar Venkatachalam
Keyword(s):  
Phenolic Compounds ◽  
Octanoic Acid ◽  
Vaccenic Acid ◽  
Phytochemical Screening ◽  
Process Variables ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Bioactive Component ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In the present work, ultrasound-assisted extraction (UAE) was employed to extract the phenolic compounds from wood apple pulp. A Box-Behnken design was used to optimize the process variables. The results revealed that UAE have significantly higher TPC (17.41?0.13 mg GAE/g dW) and TFC (6.73?0.19 mg RE/g dW) in comparison with solvent extraction that gave noticeably lower TPC (12.01?0.17 mg GAE/g dW) and TFC = 4.47?0.09 mg RE/g dW). Optimally obtained extracts of wood apple pulp were subjected to phytochemical screening and it was found that bicyclo[2.2.1]heptane, 2-(1-buten-3-yl)-serverogenin acetate, 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, L-(+)- -ascorbic acid 2,6-dihexadecanoate, cis-vaccenic acid, thiopene, 2-propyl- -phenol, 2,4-bis(1,1-dimethylethyl) and octanoic acid were present only in UAE extracts. Since the bioactive component presence is remarkably higher in UAE compared to other techniques, UAE seems to be a better method for the extraction of phenolic compounds from wood apple pulp.

Box–Behnken design applied to ultrasound-assisted extraction for the determination of polycyclic aromatic hydrocarbons in river sediments by gas chromatography/mass spectrometry

2014 ◽  
Vol 6 (6) ◽  
pp. 1650-1656 ◽  
Author(s):  
Mellina D. R. Santos ◽  
Marcos R. F. Cerqueira ◽  
Marcone A. L. de Oliveira ◽  
Renato C. Matos ◽  
Maria A. C. Matos
Keyword(s):  
Aromatic Hydrocarbons ◽  
River Sediments ◽  
Gas Chromatography Mass Spectrometry ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Ultrasonic Bath ◽  
Assisted Extraction ◽  
Polycyclic Aromatic ◽  
Ultrasound Assisted

This paper describes the optimization of the extraction of PAHs in sediment samples from a river by applying an ultrasonic bath for quantification by GC/MS.

Box–Behnken design applied to optimize the ultrasound-assisted extraction of petroleum biomarkers in river sediment samples using green analytical chemistry

2017 ◽  
Vol 9 (40) ◽  
pp. 5859-5867 ◽  
Author(s):  
Fausto M. Araujo ◽  
Mellina D. R. Santos ◽  
Marcone A. L. de Oliveira ◽  
Renato C. Matos ◽  
Maria. A. C. Matos
Keyword(s):  
Analytical Chemistry ◽  
River Sediment ◽  
Green Analytical Chemistry ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Sediment Samples ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In this paper, the Box–Behnken design was used to optimize the conditions for the ultrasound-assisted extraction of petroleum biomarkers in sediments. The optimized method is an example of a greener analytical chemistry.

Box–Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide

2013 ◽  
Vol 92 (1) ◽  
pp. 604-611 ◽  
Author(s):  
J. Prakash Maran ◽  
S. Manikandan ◽  
K. Thirugnanasambandham ◽  
C. Vigna Nivetha ◽  
R. Dinesh
Keyword(s):  
Statistical Modeling ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Corn Silk ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals Ultrasound-assisted Extraction of Polysaccharide from Radix Bupleuri using Response Surface Methodology and Antioxidant Research of the Polysaccharide

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Changchun Hao ◽  
Hongjin Qu ◽  
Shi Chen ◽  
Wenjing Han ◽  
Runguang Sun
Keyword(s):  
Response Surface Methodology ◽  
Hydroxyl Radical ◽  
Dilution Rate ◽  
Response Surface ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Radix Bupleuri ◽  
Ultrasound Assisted

This report used Response Surface Methodology (RSM) to analyze the conditions that extracting polysaccharide compound from Radix Bupleuri using ultrasonic wave. For determining the optimal extraction conditions, four independent variables, temperature (°C), ultrasonic intensity (UI, W/cm2), dilution rate (water-material ratio, mL/g) and time (min), were investigated using Box-Behnken design. In the results, the best condition after optimization was dilution rate of 35.0:1, UI of 24.5 W/cm2, temperature of 69.5°C and time of 14.6 min. Extraction efficiency was 7.67% and the purity reached 44.14%. It illustrates that the ultrasound-assisted extraction (UAE) was efficient and suitable for extracting polysaccharide from Radix Bupleuri. This paper also investigated the antioxidant activity of Radix Bupleuri polysaccharide (RBP) on superoxide anion radical, hydroxyl radical, and 2,2-diphenyl-1-picry-hydrazyl (DPPH), in vitro experiment. The result indicated that RBP could remarkably sweep away the hydroxyl radical, and had the capacity for clearing away superoxide radical and DPPH.

Innovative Extraction Techniques for the Optimum Extraction of Phenolic Compounds from Peanut Meal and Evaluation of Their Biological Activities

2019 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Phenolic Compounds ◽  
Carcinoma Cell ◽  
Biological Activities ◽  
Peanut Meal ◽  
Ultrasound Assisted Extraction ◽  
Carcinoma Cell Lines ◽  
Assisted Extraction ◽  
Phenolic Extract ◽  
Ultrasound Assisted ◽  
Water Ratio

There is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. This work aimed at extracting phenolic compounds from peanut meal. The methods of extraction were mainly: conventional solvent extraction (traditional methods) and ultrasound assisted extraction (recent methods) and comparing their results. Peanut meal (PM) was prepared by defatting with n-hexane, and then extracted by the two previous methods. First, the conventional solvents used were 80% methanol, ethanol, acetone, isopropanol, and distilled water. Then studied Different parameters such as meal: water ratio, also the effect of temperature and the pH on the extraction process. Second, ultrasonic assisted extractions (USAE), the parameters investigated were temperature, time and speed of sonication. Finally, all the extracts were analyzed by HPLC for their phenolic contents. Results indicated that the highest extracted PC achieved by solvents was in distilled water where 1:100, Meal: Water ratio which extracted 40 mg PC / g PM at 30& 35°C. Highest extracted PC was achieved by alkaline medium at pH 12 more than acidic and neutral medium. While (USAE) at speed 8 ultrasonication and temperature 30ᵒC, extracted 49.2mg PC /g PM. Sothe ultrasound assisted extraction exhibited great influence on the extraction of phenolic compounds from peanut meal. The ultrasonic peanut extract was examined for its antioxidant, antimicrobial and anticarcinogenic activities. The antioxidant activity of PM phenolic extract prepared by ultrasonic technique, was measured by, β-carotene, and DPPH methods, and reducing antioxidant power. Results revealed values: 84.57, 57.72 and 5960 respectively. The PM extract showed different levels of antimicrobial activity against the pathogenic bacteria used. As for the anticarcinogenic effect PM phenolic extract most effective on inhibiting colon carcinoma and lung carcinoma cell lines with IC50 = 20.7 and 20.8 µ/ml., respectively. This was followed by intestinal carcinoma and liver carcinoma cell lines with IC50= 39.6 and 40.2µ/ml.

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