Simple and Reliable Methods for the Determination of Three Steroidal Glycosides in the Eight Species of Solanum by Reversed-phase HPLC Coupled with Diode Array Detection

2012 ◽  
Vol 24 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Anupam Maurya ◽  
N. Manika ◽  
Ram Kishore Verma ◽  
Subhash C. Singh ◽  
Santosh Kumar Srivastava
Keyword(s):  
Reversed Phase ◽  
Diode Array ◽  
Diode Array Detection ◽  
Reversed Phase Hplc ◽  
Steroidal Glycosides ◽  
Array Detection ◽  
Reliable Methods

Amisulpride poisoning: a report on two cases

1995 ◽  
Vol 14 (3) ◽  
pp. 294-298 ◽  
Author(s):  
A. Tracqui ◽  
C. Mutter-Schmidt ◽  
P. Kintz ◽  
C. Berton ◽  
P. Mangin
Keyword(s):  
Qt Interval ◽  
Reversed Phase ◽  
Diode Array ◽  
Diode Array Detection ◽  
Reversed Phase Hplc ◽  
Drug Determination ◽  
Substituted Benzamides ◽  
Array Detection ◽  
Human Poisoning ◽  
Extrapyramidal Features

The first two observations of human poisoning involving the recently developed neuroleptic amisulpride are described. In both cases drug determination was per formed using reversed-phase HPLC coupled with diode- array detection. Case 1 was a nonfatal overdosage in which the ingestion of 3.0 g amisulpride induced an attack of seizures, then light coma with agitation, hyperthermia, mydriasis, minimal extrapyramidal features, tachycardia and slight prolongation of the QT interval; the blood con centration of amisulpride was 9.63 μg ml-1. Case 2 was a fatality attributed to amisulpride in which the measured blood concentration was 41.70 μg ml-1. Our results are discussed in the light of data previously reported on the toxicity of substituted benzamides.

Determination of Curcuminoids by Liquid Chromatography with Diode Array Detection: Application to the Characterization of Turmeric and Curry Samples

2020 ◽  
Vol 16 (2) ◽  
pp. 95-105 ◽  
Author(s):  
Òscar Vidal ◽  
Xavier Castilla ◽  
Núria Aliaga-Alcalde ◽  
Ana M. López-Periago ◽  
Concepción Domingo ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Diode Array ◽  
Diode Array Detection ◽  
Experimental Conditions ◽  
Gradient Based ◽  
Peak Areas ◽  
Array Detection

Background: A simple, rapid and efficient method for the determination of curcumin and other polyphenols in turmeric and curry samples was here developed. The method relied on sample extraction with methanol and extract analysis by liquid chromatography with diode array detection (HPLC-DAD). Methods: The separation of components was carried out in reversed-phase mode using an elution gradient based on 0.1% (v/v) formic acid aqueous solution and acetonitrile as the components of the mobile phase. Chromatograms were recorded at 420 nm for specific monitoring of curcumin and related compounds. Results: Extraction and separation conditions were optimized by experimental design and multicriteria response functions. Figures of merit were established under the selected experimental conditions. In general, repeatability of peak areas were better than 0.4%, detection limits were below 0.006 mg L-1 and quantitative recoveries expressed as a percentage were about 100 ± 2. The method was applied to quantify curcuminoids in commercial samples. It was found that apart from curcumin, demethoxycurcumin and bisdemethoxycurcumin, other related molecules also occurred in the samples. In this regard, a tentative elucidation of possible unknown curcuminoids was attempted by liquid chromatography coupled to mass spectrometry. Conclusion: Differences in the compositional profiles among samples were encountered to be relevant, so that the resulting HPLC-DAD data was exploited for chemometric characterization of turmeric and curry samples. Samples were successfully discriminated according to matrix types, species varieties and origins.

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