Free Radical Scavenging Activities of Polyphenolic Compounds Isolated from Medicago sativa and Medicago truncatula Assessed by Means of Thin-layer Chromatography DPPH˙ Rapid Test

2012 ◽  
Vol 24 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Łukasz Cieśla ◽  
Iwona Kowalska ◽  
Wiesław Oleszek ◽  
Anna Stochmal
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Medicago Sativa ◽  
Thin Layer Chromatography ◽  
Radical Scavenging ◽  
Rapid Test ◽  
Free Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Layer Chromatography ◽  
Free Radical Scavenging Activities

scholarly journals Triterpenes from Warszewiczia coccinea (Rubiaceae) as Inhibitors of Acetylcholinesterase

2009 ◽  
Vol 4 (10) ◽  
pp. 1934578X0900401
Author(s):  
Angela I. Calderón ◽  
Johayra Simithy ◽  
Giuliana Quaggio ◽  
Alex Espinosa ◽  
Jose Luis López-Pérez ◽  
...  
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
Ache Inhibitors ◽  
Ache Inhibitory Activity ◽  
Stem Extract ◽  
Layer Chromatography ◽  
Free Radical Scavenging Activities

Seventy-six plant extracts from the Panamanian flora have been screened for acetylcholinesterase (AChE) inhibitors by thin-layer chromatography (TLC) bioautography. The most promising extracts with AChE inhibitory and free radical scavenging activities at 100 μg were those of Tabernaemontana panamensis (Markgr., Boiteau & L. Allorge) Leeuwenb., Pentagonia macrophylla Benth., and Warszewiczia coccinea (Vahl) Klotzsch. Bioguided fractionation of W. coccinea stem extract afforded two triterpenes, 3β,6β,19α-trihydroxy-urs-12-en-28-oic acid (1) and 3β,6β-dihydroxy-olean-12-en-28-oic acid (sumaresinolic acid) (2), with AChE inhibitory activity. Their structures were determined by spectroscopic methods. This is the first report of these bioactive triterpenes in W. coccinea.

scholarly journals HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY ANALYSIS AND FREE RADICAL SCAVENGING POTENTIAL OF SOUTH INDIAN ORTHODOX BLACK TEA

2018 ◽  
Vol 11 (9) ◽  
pp. 449
Author(s):  
Mahalakshmi Rajamani ◽  
Kalaivani Krishnasamy ◽  
Nishadh Abubakker
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Hydroxyl Radical ◽  
High Performance ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Black Tea ◽  
Free Radical Scavenging ◽  
South Indian ◽  
Layer Chromatography

Objective: The objective of this study was to evaluate the free radical scavenging potential and high-performance thin-layer chromatography (HPTLC) fingerprinting of the ethanolic extract of south Indian orthodox black tea (OBT).Methods: Phytochemical analysis was carried out using standard methods, and free radical scavenging activity of the extract was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), superoxide anion (SOD), and hydroxyl radical scavenging capacities. The ethanolic extract of OBT was loaded in the pre-coated HPTLC plates (silica gel 60 F 254) E-MERCK KGaA. HPTLC was carried out with toluene: ethyl acetate: diethylamine (7:2:1), chloroform: methanol:formic acid (8.5:1.0:0.5), and butanol: isopropyl alcohol (1:1) as mobile phase for alkaloids, flavonoids, and terpenoids, respectively.Results: HPTLC results confirmed that the extract contained several potential active components such as flavonoids, alkaloids, and terpenoids as the slides revealed multicolored bands of varying intensities. Extract of OBT reliably showed the total phenolics 132.27 mg/g, flavonoids 72.52 mg/g, and alkaloids 66.01 mg/g of dry matter. The IC50 value of OBT for DPPH was found to be 372.22 μg/ml, SOD 311.93 μg/ml, NO 362.17 μg/ml, hydroxyl radical 342.14 μg/ml, and reducing power 178.54 μg/ml.Conclusion: The HPTLC fingerprinting profile developed for ethanolic extract will help in proper identification and quantification of marker compounds. The ethanolic extract of OBT was found to possess a wide range of phytochemicals with excellent antioxidant properties. This information may help to choose the best beverage to be consumed in the future.

scholarly journals PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITIES OF MEDICAGO SATIVA SEEDS

2015 ◽  
Vol 2 (1) ◽  
pp. 128-132
Author(s):  
Gomathi R ◽  
Banu S ◽  
Usha K
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Medicago Sativa ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Free Radical Scavenging Activities

Phytochemical analysis and in vitro free radical scavenging activities were analyzed in the various extracts of Medicago sativa seeds. The phytochemical analysis showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, saponins, phytosterols, tannins, terpenoids and phenols. Among the various extracts, phytochemicals were extracted best in ethanol. Free radical scavenging activities such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, superoxide, 2,2'-azino-bis (3-ethylbenzothiazoline-6- sulphonic acid) (ABTS), ferrous ion chleating activity and non radicals such as hydrogen peroxide and nitric oxide were analyzed in the various extracts of Medicago sativa seeds and were compared with standard antioxidant ascorbic acid. All the extracts of Medicago sativa seeds scavenged the free radicals in a concentration dependent manner. The antioxidative activity of all the extracts was found to be more pronounced than that of the standard antioxidant ascorbic acid. Among the various extracts, the antioxidant activity was found to be more pronounced in ethanolic extract of Medicago sativa seeds

Polyphenolic compounds from Geranium pratense and their free radical scavenging activities

2001 ◽  
Vol 56 (2) ◽  
pp. 189-193 ◽  
Author(s):  
Zeliha Ş. Akdemir ◽  
İ. İrem Tatlı ◽  
İclal Saracoğlu ◽  
U.Burçin İsmailoğlu ◽  
İnci Şahin-Erdemli ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Free Radical Scavenging Activities
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