Development of chromatographic and free radical scavenging activity fingerprints by thin-layer chromatography for selected Salvia species

2010 ◽  
Vol 22 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Łukasz Cieśla ◽  
Dorota Staszek ◽  
Michał Hajnos ◽  
Teresa Kowalska ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Layer Chromatography

scholarly journals NMR characterization and free radical scavenging activity of pheophytin ‘A’ from the leaves of Dissotis rotundifolia

2021 ◽  
Vol 35 (1) ◽  
pp. 207-215
Author(s):  
C. Friday ◽  
O. UchennaIgwe ◽  
U. C. Akwada
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Pheophytin A ◽  
Chromatographic Techniques ◽  
Nmr Characterization

Dissotis rotundifolia is used traditionally in various parts of Africa for the treatment of various ailments. The air dried and pulverized leaves of Dissotis rotundifolia was extracted with distilled n-hexane and ethylacetate, using Soxhlet apparatus. Isolation and purification were carried out using column and thin layer chromatographic techniques. Fraction B14 gave a single spot on thin layer chromatography with a retention factor (Rf) value of 0.57. Characterization of B14 was doneusing 1H-NMR, 13C-DEPT, COSY, HSQC, HMBC and by direct comparison with literature values and pheophytin A was proposed as the structure of the compound. Pheophytin A exhibited a free radical scavenging activity of 10.10±0.05 and 19.51±0.02% at minimum and maximum concentrations of 1.00 and 4.00 mg/mL, respectively. The acclaimed ethnomedicinal uses of D. rotundifolia by the African natives could be linked to the presence of pheophytin A and other phytoconstituents in the plant. This is the first report of the isolation of pheophytin A from D. rotundifolia.                     KEY WORDS: Dissotis rotundifolia, Pheophytin A, Chromatographic techniques, Free radicals, NMR   Bull. Chem. Soc. Ethiop. 2021, 35(1), 207-215. DOI: https://dx.doi.org/10.4314/bcse.v35i1.18      

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