Cell proliferation correlates with prognosis in acinic cell carcinomas of salivary gland origin. Immunohistochemical study of 30 cases using the MIB 1 antibody in formalin-fixed paraffin sections

1994 ◽  
Vol 173 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Alena Skalova ◽  
Ilmo Leivo ◽  
Kristina Von Boguslawsky ◽  
Eero Saksela
Keyword(s):  
Cell Proliferation ◽  
Salivary Gland ◽  
Immunohistochemical Study ◽  
Paraffin Sections ◽  
Acinic Cell ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed ◽  
Mib 1

Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections

1992 ◽  
Vol 168 (4) ◽  
pp. 357-363 ◽  
Author(s):  
Giorgio Cattoretti ◽  
Michael H. G. Becker ◽  
Göran Key ◽  
Michael Duchrow ◽  
Carsten Schlüuter ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Paraffin Sections ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed ◽  
Mib 1

scholarly journals Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections.

1993 ◽  
Vol 41 (11) ◽  
pp. 1599-1604 ◽  
Author(s):  
S R Shi ◽  
B Chaiwun ◽  
L Young ◽  
R J Cote ◽  
C R Taylor
Keyword(s):  
Androgen Receptor ◽  
Enzymatic Digestion ◽  
Antigen Retrieval ◽  
Urea Solution ◽  
Paraffin Sections ◽  
Buffer Solution ◽  
Solution Ph ◽  
Citrate Buffer ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed

We developed a staining protocol for demonstration of androgen receptor (AR) in formalin-fixed, paraffin-embedded tissue sections. The method is based on the antigen retrieval microwave (MW) heating technique. Results are compared with different types of enzyme digestion pre-treatments. The strongest immunostaining signal and clearest background were obtained by MW heating of dewaxed paraffin sections in 5% urea or citrate buffer solution (pH 6); pure distilled water gave less consistent results. Enzymatic digestion with pepsin (0.05% in 2 N HCl) for 30 min at room temperature, or trypsin followed by pronase, or pronase digestion alone, also produced enhanced staining of AR in some cases, but there was more nonspecific background, and specific reactivity was less intense. The antigen retrieval MW method can be used to demonstrate AR epitope in prostate tissue after fixation in formalin for as long as 7 days. AR immunolocalization was also compared in frozen and paraffin sections processed from the same specimen of prostate carcinoma tissue and was found to be qualitatively and quantitatively similar. This study also provided new information concerning the basic principles of the antigen retrieval MW method that may be helpful in further development of this technique.

Astrocytes in Formalin-Fixed Paraffin Sections

1969 ◽  
Vol 51 (3_ts) ◽  
pp. 416-419 ◽  
Author(s):  
Anthony G. H. Folliss ◽  
Martin G. Netsky
Keyword(s):  
Paraffin Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed

Immunohistochemical Detection of Canine Adenovirus in Paraffin Sections of Liver

1986 ◽  
Vol 23 (4) ◽  
pp. 478-484 ◽  
Author(s):  
P. M. Rakich ◽  
K. W. Prasse ◽  
P. D. Lukert ◽  
L. M. Cornelius
Keyword(s):  
Immunohistochemical Detection ◽  
Hepatic Inflammation ◽  
Paraffin Sections ◽  
Inflammatory Lesions ◽  
Long Term Stability ◽  
Formalin Fixed Paraffin ◽  
Wide Range ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

An avidin-biotin immunoperoxidase procedure was optimized for detection of canine adenoviral antigens in formalin-fixed, paraffin-embedded liver. Long-term stability of viral antigen was shown by successful demonstration of virus in liver tissue preserved up to six years from dogs with infectious canine hepatitis. This immunohistochemical stain was applied to sections from livers with a wide range of inflammatory lesions. Examination of sections from 53 dogs yielded five livers with small amounts of adenovirus. An additional virus-positive liver was identified from a dog with no hepatic inflammation. Although a cause and effect relationship remains to be determined, these findings suggest a possible connection between canine adenovirus and spontaneous chronic hepatitis.

Immunocytochemical p53 detection by microwave oven heating of routinely formalin-fixed paraffin sections

1993 ◽  
Vol 171 (2) ◽  
pp. 151-152 ◽  
Author(s):  
Daniela Campani ◽  
Denise Cecchetti ◽  
Generoso Bevilacqua
Keyword(s):  
Microwave Oven ◽  
Paraffin Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed
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