Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some, neoplasms

1990 ◽  
Vol 162 (4) ◽  
pp. 285-294 ◽  
Author(s):  
P. A. Hall ◽  
D. A. Levison ◽  
A. L. Woods ◽  
C. C.-W. Yu ◽  
D. B. Kellock ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Paraffin Sections ◽  
Proliferating Cell

scholarly journals Proliferating cell nuclear antigen (PCNA): a new marker to study human colonic cell proliferation.

Gut ◽  
1994 ◽  
Vol 35 (4) ◽  
pp. 530-535 ◽  
Author(s):  
F J Kubben ◽  
A Peeters-Haesevoets ◽  
L G Engels ◽  
C G Baeten ◽  
B Schutte ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Proliferating Cell

scholarly journals Proliferating Cell Nuclear Antigen as the Cell Cycle Sensor for an HLA-Derived Peptide Blocking T Cell Proliferation

2000 ◽  
Vol 164 (12) ◽  
pp. 6188-6192 ◽  
Author(s):  
Xuefeng Ling ◽  
Salar Kamangar ◽  
Michelle L. Boytim ◽  
Zvi Kelman ◽  
Philip Huie ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
T Cell ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
T Cell Proliferation ◽  
Proliferating Cell

R403 – Epidermal Proliferating Cell Nuclear Antigen in Aging Rats

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P178-P178 ◽  
Author(s):  
Tapan K Bhattacharyya ◽  
Paula Jackson ◽  
J Regan Thomas
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Proliferation Index ◽  
Fischer 344 Rats ◽  
Fischer 344 ◽  
Age Related ◽  
Two Parameters ◽  
Proliferating Cell ◽  
Effect Of Age

Problem To determine if epidermal cell proliferation in colony-raised Fischer 344 rats changes with age and diet. Methods Fischer 344 rats fed ad libitum and calorie-restricted (CR) diets were obtained from the NIA colonies, and young, young adult, and old animals from both groups were used for this study (six in each group). Tissue sections from the dorsal skin (DS) and foot plate (FP) were used for immunohistochemical staining of proliferating cell nuclear antigen (PCNA). The proliferation index (PCNA-I) was computed from counts of stained and total number of keratinocytes. Simultaneous measurements of epidermal thickness were obtained from same sections. Data were analyzed with Excel and SPSS 14.0 software for statistics. Results Two-way analysis of variance (ANOVA) was applied to the data to probe the effect of age, diet, and age-diet interaction. A significant effect of age was noticed in the two parameters i.e., DS PCNA (F 3.96, P .011), FP epidermal width (F 3.37, P .021) and FP PCNA-I (F 9.0, P .000). A significant correlation between DS width and PCNA values was also noted (r 0.5, P .01). Conclusion There is a trend of reduction of PCNA positive cells with increasing age irrespective of thickness of epidermis, and this trend is more apparent in CR rats. Significance This cell proliferation study has implications in relation to CR effect on age-related disease conditions, and biogerontology. Support The study was partially funded by the 2007 Leslie Bernstein grant from AAFPRS foundation.

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