Glomerular changes in guinea-pig kidneys produced by rabbit anti-sheep-red-cell serum (Forssman antibody)

1961 ◽  
Vol 82 (2) ◽  
pp. 517-519 ◽  
Author(s):  
Faith Maurice-Williams ◽  
R. S. Morgan ◽  
G. Payling Wright
Keyword(s):  
Guinea Pig ◽  
Red Cell ◽  
Forssman Antibody

scholarly journals THE AGGLUTINATION OF RED BLOOD CELLS IN THE PRESENCE OF BLOOD SERA

1922 ◽  
Vol 4 (4) ◽  
pp. 403-409 ◽  
Author(s):  
Calvin B. Coulter
Keyword(s):  
Cell Membrane ◽  
Guinea Pig ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Ion Concentration ◽  
Red Cell ◽  
Free Medium ◽  
Optimal Point ◽  
Reaction Characteristic ◽  
Pig Serum

1. The addition of blood serum displaces the optimum for agglutination of red blood cells in a salt-free medium to the reaction characteristic of flocculation of the serum euglobulin. 2. This effect is not due merely to a mechanical entanglement of the cells by the precipitating euglobulin, since at reactions at which the latter is soluble it protects the cells from the agglutination which occurs in its absence. 3. A combination of some sort appears therefore to take place between sheep cells and sheep, rabbit, and guinea pig serum euglobulin, and involves a condensation of the serum protein upon the surface of the red cell. 4. At the optimal point for agglutination of persensitized cells both mid- and end-piece of complement combine with the cells. 5. Agglutination is closely related to an optimal H ion concentration in the suspending fluid, and probably of the cell membrane, and not to a definite reaction in the interior of the cell.

Thrombocytopaenia, Thromboxane And Prostacyclin Production During The Forssman Reaction In The Guinea-Pig: Effect Of A Thromboxane Synthetase Inhibitor

1981 ◽  
Author(s):  
J Westwick ◽  
K Butler ◽  
A Honey ◽  
S Krishnamurthi ◽  
V V Kakkar
Keyword(s):  
Guinea Pig ◽  
Post Injection ◽  
Blood Samples ◽  
Synthetase Inhibitor ◽  
Thromboxane Synthetase Inhibitor ◽  
Thromboxane Synthetase ◽  
Thromboxane Production ◽  
Antigen Antibody ◽  
Prostacyclin Production ◽  
Forssman Antibody

Intra venous (iv) injection of Forssman antibody into the guinea-pig (GP) is known to result in a rapid antigen antibody reaction in the lungs leading to bronchospasm and thrombocytopaenia. Death as a result of Forssman shock has been shown to be complement and platelet dependentGroups of 5 Duncan-Hartley GP (350-400g) received either 0.2ml (sub lethal groups) or 0.6ml (lethal groups) of rabbit anti Forssman antiserum (RAFA) iv, then 5ml blood samples were collected via carotid artery cannula, into 0.5ml ice cold EDTA (lOOmM) and indomethacin (180μM) mixture at 0, 1, 3 and 5 min. post injection. The blood samples were rapidly centrifuged at 15,000g for 3 min, plasma removed and frozen at -20°C until assayed. The plasma was then assayed for TxB2 and 6-oxo-PGF1α using specific RIA’s. There was a marked TxB2 production during the thrombocytopaenia with a concomitant small increase in 6-oxo-PGF1α production.However, when groups of 5 GPs were dosed iv with 0, 1, 3 or 10 mg/kg of UK-37, 248, a potent and selective thromboxane synthetase inhibitor, 3 mins prior to 0.2ml of RAFA the resulting thrombocytopaenia was not inhibited at 0, 0.5, 1, 2, 5, 10 and 15 min post RAFA. Thus thromboxane production does not appear to be contributory to the Forssman induced thrombocytopaenia in the GP.

Effects of forssman antibody on renal function of the guinea pig

1987 ◽  
Vol 187 (6) ◽  
pp. 407-414 ◽  
Author(s):  
J. E. Barbosa ◽  
W. Sarti ◽  
G. B. Mello
Keyword(s):  
Renal Function ◽  
Guinea Pig ◽  
Forssman Antibody
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