Fluorescence lifetime imaging of unstained tissues: early results in human breast cancer

2003 ◽  
Vol 199 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Paul J Tadrous ◽  
Jan Siegel ◽  
Paul MW French ◽  
Sami Shousha ◽  
El-Nasir Lalani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fluorescence Lifetime ◽  
Human Breast Cancer ◽  
Fluorescence Lifetime Imaging ◽  
Human Breast ◽  
Lifetime Imaging ◽  
Early Results

scholarly journals Automated detection of breast cancer in resected specimens with fluorescence lifetime imaging

2017 ◽  
Vol 63 (1) ◽  
pp. 015003 ◽  
Author(s):  
Jennifer E Phipps ◽  
Dimitris Gorpas ◽  
Jakob Unger ◽  
Morgan Darrow ◽  
Richard J Bold ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fluorescence Lifetime ◽  
Automated Detection ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Metabolic Changes in Maternal and Cord Blood in One Case of Pregnancy-Associated Breast Cancer Seen by Fluorescence Lifetime Imaging Microscopy

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1494
Author(s):  
Li Zhou ◽  
Yawei Kong ◽  
Junxin Wu ◽  
Xingzhi Li ◽  
Yiyan Fei ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cord Blood ◽  
Fluorescence Lifetime ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Potential Method ◽  
Metabolic Changes ◽  
Diagnostic Methods ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Pregnancy-associated breast cancer (PABC) is a rare disease, which is frequently diagnosed at an advanced stage due to limitations in current diagnostic methods. In this study, fluorescence lifetime imaging microscopy (FLIM) was used to study the metabolic changes by measuring maternal blood and umbilical cord blood via the autofluorescence of coenzymes, reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), and flavin adenine dinucleotide (FAD). The NAD(P)H data showed that a PABC case had significant differences compared with normal cases, which may indicate increased glycolysis. The FAD data showed that both maternal and cord blood of PABC had shorter mean lifetimes and higher bound-FAD ratios. The significant differences suggested that FLIM testing of blood samples may be a potential method to assist in PABC non-radiative screening.

Metabolic Mapping of MCF10A Human Breast Cells via Multiphoton Fluorescence Lifetime Imaging of the Coenzyme NADH

2005 ◽  
Vol 65 (19) ◽  
pp. 8766-8773 ◽  
Author(s):  
Damian K. Bird ◽  
Long Yan ◽  
Kristin M. Vrotsos ◽  
Kevin W. Eliceiri ◽  
Emily M. Vaughan ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Human Breast ◽  
Metabolic Mapping ◽  
Lifetime Imaging ◽  
Breast Cells

Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

1973 ◽  
Vol 31 ◽  
pp. 378-379
Author(s):  
G. Kasnic ◽  
S. E. Stewart ◽  
C. Urbanski
Keyword(s):  
Breast Cancer ◽  
Biological Activity ◽  
Human Breast Cancer ◽  
Osteogenic Sarcoma ◽  
Human Tumor ◽  
Structural Similarity ◽  
Cell Tumor ◽  
Human Breast ◽  
Human Tumor Cell ◽  
Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

Further Ultrastructural Observations on Metastatic Nodules of Human Breast Cancer

1968 ◽  
Vol 26 ◽  
pp. 224-225
Author(s):  
John L. Swedo ◽  
R. W. Talley ◽  
John H. L. Watson
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Estrogen Therapy ◽  
Specimen Preparation ◽  
Human Breast ◽  
Autophagic Vacuoles ◽  
Previous Communication ◽  
Linear Profiles ◽  
Metastatic Nodule

Since the report, which described the ultrastructure of a metastatic nodule of human breast cancer after estrogen therapy, additional ultrastructural observations, including some which are correlative with pertinent findings in the literature concerning mycoplasmas, have been recorded concerning the same subject. Specimen preparation was identical to that in.The mitochondria possessed few cristae, and were deteriorated and vacuolated. They often contained particulates and fibrous structures, sometimes arranged in spindle-shaped bundles, Fig. 1. Another apparent aberration was the occurrence, Fig. 2 (arrows) of linear profiles of what seems to be SER, which lie between layers of RER, and are often recognizably continuous with them.It was noted that the structure of the round bodies, interpreted as within autophagic vacuoles in the previous communication, and of vesicular bodies, described morphologically closely resembled those of some mycoplasmas. Specifically, they simulated or reflected the various stages of replication reported for mycoplasmas grown on solid nutrient. Based on this observation, they are referred to here as “mycoplasma-like” structures, in anticipation of confirmatory evidence from investigations now in progress.

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